This Project has set important technological milestones and made several breakthrough discoveries in the field of plant biology and cell wall sensing and integrity. For the scientific community we have set a new expression and purification protocol to produce folded small cys-rich proteins. Similarly, we have also established solid methods to measure binding with carbohydrates using different methods as well as measuring different rheology properties of artificial cell walls. We have also uncovered the recognition mechanisms of a family of peptides by two distinct families (Moussu et al., PNAS, 2020), we have also uncovered how plant cells molecularly pattern their cell wall to sustain cell expansion (Moussu et al., Acta D, 2018; Moussu, Lee et al., Science, 2023; Schoenaers, Lee et al.,, Nat Plants, in press) and we have discovered new cell wall receptors that are involved in enhancing plant immunity against several pathogens. In addition, we have dissected molecularly distinct receptor-peptide recognition mechanisms that ensure signal specificity in cells (Roman, Jímenez-Sandoval et al., Nat Commns, 2021) and we have contributed to shed light in multiple cell wall and cell signaling pathways through different collaborations, promoting a synergistic scientific network.