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Boosting tryptophan fluorescence with optical nanoantennas to watch label-free protein dynamics with single molecule resolution at high concentration

Objective

Proteins execute a broad range of functions that are central to life. Understanding these functions ultimately requires experiments at the single protein level to reveal dynamics and heterogeneities hidden in ensemble-averaged measurements. Currently, the preferred method to study single protein machineries is based on fluorescence techniques. However, fluorescence experiments suffer from major challenges: the need for external fluorescent labeling, weak signals and low non-physiological concentrations in the nanomolar range. These challenges severely limit the applicability and biological relevance of single molecule fluorescence on proteins. The TryptoBoost project aims to overcome all the previous challenges, and efficiently monitor single label-free proteins using their intrinsic tryptophan fluorescence enhanced by optical nanoantennas in the ultraviolet. Using the natural amino acid fluorescence rules out all drawbacks due to external labeling, while the optical nanoantennas enable single protein analysis at the physiologically relevant micromolar concentrations thanks to the localization and enhancement of light-matter interactions at the nanoscale. To demonstrate the power of this new technology, our interdisciplinary team will probe the important biological problems of amyloid aggregation and tumor suppressor p53 protein folding dynamics at high concentrations. The TryptoBoost approach is directly applicable to any protein containing aromatic amino acids. This condition is met by more than 90% of human proteins, so the project breakthroughs will benefit a broad range of biophysical, chemical, and medical applications. For instance, it will improve the development of therapeutic drugs, increase the detection sensitivity and read-out speed in analytical biosensing on chip, and provide new nanostructures to enhance ultraviolet photocatalysis.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-COG - Consolidator Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2016-COG

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Host institution

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 947 208,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 947 208,13

Beneficiaries (1)

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