Decoding mitochondrial nutrient-sensing programs in POMC neurons as key determinants of metabolic health

Informations projet

MITOSENSING

N° de convention de subvention: 725004

DOI

10.3030/725004

Projet clôturé

Date de signature de la CE 22 Février 2017

Date de début 1 Avril 2017

Date de fin 30 Septembre 2022

Financé au titre de

• EXCELLENT SCIENCE - European Research Council (ERC)

Coût total

€ 1 999 573,00

Contribution de l’UE

€ 1 999 573,00

Coordonné par

FUNDACIO DE RECERCA CLINIC BARCELONA-INSTITUT D INVESTIGACIONS BIOMEDIQUES AUGUST PI I SUNYER
Spain

Periodic Reporting for period 4 - MITOSENSING (Decoding mitochondrial nutrient-sensing programs in POMC neurons as key determinants of metabolic health)

Période du rapport: 2021-10-01 au 2022-09-30

Résumé du contexte et des objectifs généraux du projet

Obesity has reached epidemic dimensions worldwide causing major human and economic repercussions. However, and despite the magnitude of this health problem, non-invasive treatments are scarce and insufficient in terms of efficacy. This is the consequence of our limited understanding of the molecular mechanisms underlying energy balance control.

Nutrient-sensing by hypothalamic neurons is a crucial process to monitor the metabolic status of the organism and to coordinate adaptive responses to maintain energy homeostasis. Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus are prototypical cellular sensors and key regulators of appetite, energy expenditure and metabolism. The hypothesis of MITOSENSING is that alterations in specific mitochondrial nutrient-sensing programs in POMC neurons cause energy balance defects that underlie the development of obesity and associated metabolic disorders such as type-2 diabetes (T2D).

The objectives are: 1) to identify transcriptionally-modulated mitochondrial nutrient-sensing programs in POMC neurons; 2) to investigate whether disruption of specific nutrient-sensing programs in POMC neurons cause metabolic disorders; 3) to investigate whether the development of lifestyle-associated metabolic disorders are caused by defective mitochondrial nutrient-sensing programs in POMC neurons.

Collectively, the results derived from this action evidenced that mitochondria in POMC neurons act as key signaling platforms integrating appetite, the metabolic function of peripheral tissues and cognitive performance.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

We have established the transcriptional signatures induced by glucose, Leucine and oleic acid in POMC neurons. We have also investigated the role of diverse mitochondrial functions in POMC neurons upon metabolic health. Our studies have revealed novel and unsuspected facets at the interface of appetite, peripheral metabolism control and cognitive performance. Furthermore, our research has also identified alterations in distinct transcriptional signatures of POMC neurons, in neonatal and perinatal developmental stages, associated with maternal obesogenic conditions. In conclusion, this action has provided novel insights on the role of mitochondria in POMC neurons in the context of highly prevalent metabolic conditions such as obesity and type-2 diabetes.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

MITOSENSING will represent a big step forward into the understanding of neuron type-specific nutrient-sensing programs in the context of obesity and metabolic alterations. The identification of such networks will open new and unexpected lines of investigation with potential therapeutical implications for obesity and T2D.

POMC neurons (green) in the arcuate nucleus of the hypothalamus