Interaction and Kinetics of Oxidative Biomass Degrading Enzymes Resolved by High-Resolution Techniques

Project Information

OXIDISE

Grant agreement ID: 726396

DOI

10.3030/726396

Project closed

EC signature date 21 February 2017

Start date 1 March 2017

End date 28 February 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 929 319,00

EU contribution

€ 1 929 319,00

Coordinated by

UNIVERSITAET FUER BODENKULTUR WIEN
Austria

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (24)

Non-productive binding of cellobiohydrolase i investigated by surface plasmon resonance spectroscopy

Author(s): Florian Csarman, Claudia Gusenbauer, Lena Wohlschlager, Gijs van Erven, Mirjam A. Kabel, Johannes Konnerth, Antje Potthast, Roland Ludwig
Published in: Cellulose, Issue 28/15, 2021, Page(s) 9525-9545, ISSN 0969-0239
Publisher: Blackie Academic & Professional
DOI: 10.1007/s10570-021-04002-6

Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei

Author(s): Lena Wohlschlager, Florian Csarman, Matea Zrilić, Bernhard Seiboth, Roland Ludwig
Published in: Enzyme and Microbial Technology, Issue 145, 2021, Page(s) 109748, ISSN 0141-0229
Publisher: Elsevier BV
DOI: 10.1016/j.enzmictec.2021.109748

Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei

Author(s): Lena Wohlschlager, Florian Csarman, Hucheng Chang, Elisabeth Fitz, Bernhard Seiboth, Roland Ludwig
Published in: Microbial Cell Factories, Issue 20/1, 2021, ISSN 1475-2859
Publisher: BioMed Central
DOI: 10.1186/s12934-020-01492-0

Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase

Author(s): Alfons K. G. Felice, Christian Schuster, Alan Kadek, Frantisek Filandr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Lorenz Schwaiger, Franziska Schachinger, Daniel Kracher, Christoph Sygmund, Petr Man, Petr Halada, Chris Oostenbrink, Roland Ludwig
Published in: ACS Catalysis, Issue 11/2, 2021, Page(s) 517-532, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.0c05294

Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola

Author(s): Florian Csarman, Tobias Obermann, Mihael Colar Zanjko, Petr Man, Petr Halada, Bernhard Seiboth, Roland Ludwig
Published in: Enzyme and Microbial Technology, Issue 148, 2021, Page(s) 109801, ISSN 0141-0229
Publisher: Elsevier BV
DOI: 10.1016/j.enzmictec.2021.109801

Insights into the H 2 O 2 ‐driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

Author(s): Tobias M. Hedison, Erik Breslmayr, Muralidharan Shanmugam, Kwankao Karnpakdee, Derren J. Heyes, Anthony P. Green, Roland Ludwig, Nigel S. Scrutton, Daniel Kracher
Published in: The FEBS Journal, Issue 288/13, 2021, Page(s) 4115-4128, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15704

Configuration of active site segments in lytic polysaccharide monooxygenases steers oxidative xyloglucan degradation

Author(s): Peicheng Sun, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Matthias Frommhagen, Dimitrios Kouzounis, Mark G. Sanders, Willem J. H. van Berkel, Roland Ludwig, Mirjam A. Kabel
Published in: Biotechnology for Biofuels, Issue 13/1, 2020, Page(s) 1-19, ISSN 1754-6834
Publisher: BioMed Central
DOI: 10.1186/s13068-020-01731-x

Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls

Author(s): Neus Gacias-Amengual; Lena Wohlschlager; Florian Csarman; Roland Ludwig
Published in: International Journal of Molecular Sciences, Issue 14220067, 2022, Page(s) Pages: 5216, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23095216

Expression and characterization of a family 45 glycosyl hydrolase from Fomitopsis pinicola and comparison to Phanerochaete chrysosporium Cel45A

Author(s): Neus Gacias Amengual, Florian Csarman, Lena Wohlschlager, Roland Ludwig
Published in: Enzyme and Microbial Technology, 2022, ISSN 0141-0229
Publisher: Elsevier BV
DOI: 10.1016/j.enzmictec.2022.110000

Amperometric Biosensors Based on Direct Electron Transfer Enzymes

Author(s): Franziska Schachinger, Hucheng Chang, Stefan Scheiblbrandner, Roland Ludwig
Published in: Molecules, Issue 26/15, 2021, Page(s) 4525, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26154525

Protein Conformational Change Is Essential for Reductive Activation of Lytic Polysaccharide Monooxygenase by Cellobiose Dehydrogenase

Author(s): Erik Breslmayr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Anita Jerkovic, Derren J. Heyes, Chris Oostenbrink, Roland Ludwig, Tobias M. Hedison, Nigel S. Scrutton, Daniel Kracher
Published in: ACS Catalysis, Issue 10/9, 2020, Page(s) 4842-4853, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.0c00754

Influence of Lytic Polysaccharide Monooxygenase Active Site Segments on Activity and Affinity

Author(s): Christophe V.F.P. Laurent, Peicheng Sun, Stefan Scheiblbrandner, Florian Csarman, Pietro Cannazza, Matthias Frommhagen, Willem J.H. van Berkel, Chris Oostenbrink, Mirjam A. Kabel, Roland Ludwig
Published in: International Journal of Molecular Sciences, Issue 20/24, 2019, Page(s) 6219, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20246219

Engineering the Turnover Stability of Cellobiose Dehydrogenase toward Long-Term Bioelectronic Applications

Author(s): Andreas F. Geiss, Thomas M. B. Reichhart, Barbara Pejker, Esther Plattner, Peter L. Herzog, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Dietmar Haltrich
Published in: ACS Sustainable Chemistry & Engineering, Issue 9/20, 2021, Page(s) 7086-7100, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.1c01165

Multiplicity of enzymatic functions in the CAZy AA3 family

Author(s): Leander Sützl, Christophe V. F. P. Laurent, Annabelle T. Abrera, Georg Schütz, Roland Ludwig, Dietmar Haltrich
Published in: Applied Microbiology and Biotechnology, Issue 102/6, 2018, Page(s) 2477-2492, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-018-8784-0

Direct Electron-Transfer Anisotropy of a Site-Specifically Immobilized Cellobiose Dehydrogenase

Author(s): Su Ma, Christophe V. F. P. Laurent, Marta Meneghello, Jani Tuoriniemi, Chris Oostenbrink, Lo Gorton, Philip N. Bartlett, Roland Ludwig
Published in: ACS Catalysis, Issue 9/8, 2019, Page(s) 7607-7615, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.9b02014

Cellobiose dehydrogenase: Bioelectrochemical insights and applications

Author(s): Stefan Scheiblbrandner, Roland Ludwig
Published in: Bioelectrochemistry, Issue 131, 2020, Page(s) 107345, ISSN 1567-5394
Publisher: Elsevier BV
DOI: 10.1016/j.bioelechem.2019.107345

Studying Direct Electron Transfer by Site‐Directed Immobilization of Cellobiose Dehydrogenase

Author(s): Marta Meneghello, Firas A. Al‐Lolage, Su Ma, Roland Ludwig, Philip N. Bartlett
Published in: ChemElectroChem, Issue 6/3, 2018, Page(s) 700-713, ISSN 2196-0216
Publisher: Wiley-VCH
DOI: 10.1002/celc.201801503

Direct Electron Transfer of Enzymes Facilitated by Cytochromes

Author(s): Su Ma, Roland Ludwig
Published in: ChemElectroChem, Issue 6/4, 2019, Page(s) 958-975, ISSN 2196-0216
Publisher: Wiley-VCH
DOI: 10.1002/celc.201801256

Characterization of pyranose oxidase variants for bioelectrocatalytic applications

Author(s): Annabelle T. Abrera, Hucheng Chang, Daniel Kracher, Roland Ludwig, Dietmar Haltrich
Published in: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Issue 1868/2, 2020, Page(s) 140335, ISSN 1570-9639
Publisher: Elsevier BV
DOI: 10.1016/j.bbapap.2019.140335

Determination of the Distance Between the Cytochrome and Dehydrogenase Domains of Immobilized Cellobiose Dehydrogenase by Using Surface Plasmon Resonance with a Center of Mass Based Model

Author(s): Jani Tuoriniemi, Lo Gorton, Roland Ludwig, Gulnara Safina
Published in: Analytical Chemistry, Issue 92/3, 2020, Page(s) 2620-2627, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.9b04490

Spectroelectrochemical investigation of the glyoxal oxidase activation mechanism

Author(s): Lena Wohlschlager, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Published in: Bioelectrochemistry, Issue 141, 2021, Page(s) 107845, ISSN 1567-5394
Publisher: Elsevier BV
DOI: 10.1016/j.bioelechem.2021.107845

Real-Time Measurement of Cellobiose and Glucose Formation during Enzymatic Biomass Hydrolysis

Author(s): Hucheng Chang, Lena Wohlschlager, Florian Csarman, Adrian Ruff, Wolfgang Schuhmann, Stefan Scheiblbrandner, Roland Ludwig
Published in: Analytical Chemistry, Issue 93/21, 2021, Page(s) 7732-7738, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c01182

Cellobiose dehydrogenase in biofuel cells

Author(s): Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Published in: Current Opinion in Biotechnology, Issue 73, 2022, Page(s) 205-212, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2021.08.013

The H2O2-dependent activity of a fungal lytic polysaccharide monooxygenase investigated with a turbidimetric assay

Author(s): Frantisek Filandr, Petr Man, Petr Halada, Hucheng Chang, Roland Ludwig, Daniel Kracher
Published in: Biotechnology for Biofuels, Issue 13/1, 2020, Page(s) 1-13, ISSN 1754-6834
Publisher: Biomed Central
DOI: 10.1186/s13068-020-01673-4