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Microbial Uptakes for Sustainable management of major bananA pests and diseases

Deliverables

Methods for large-scale cultivation of microbial EBCAs and EPNs, bioformulation and storage

Report on methods applied for the large-scale cultivation of microbial EBCAs and EPNs used in other Wps, with methods developed for formulations of EBCAs and their storage.

Data on biology and effectiveness of selected EBCAs from different regions and climates, including risk analysis data

Report on prevalence/colonization rates per isolate, biological antagonism, parasitism, host preference and other data allowing the evaluation and selection of EBCAs. Numerical results of parasitism assays carried out to evaluate BW antagonisms by microorganisms or EPN. Includes : 1) data on risk analysis for the EBCAs selected and suitable for applications in other WPs, prior to their introduction in the field, data from assays with non target invertebrates, and of dual cultures with other soil microorganisms; 2) knowledge produced on the microorganisms, shown in databases available on line (i.e. NCBI), including sequence and other data such as host colonization, target pests prevalence, and endophytism.

Report on EBCAs host-range assessment

Report summarizing the results of the greenhouse assays concerning the colonization and response of a number of banana germplasm lines tested.

Report of phytosanitary status of propagation material and evaluation of its impact on plant growth response under different IPM regimes

Report with epidemiologic data from surveyes carried out for PPN, PD and BW in nurseries concerning their phytosanitary status. Includes data on the effectiveness of EBCAs relative to conventional insecticide/nematicide use under field conditions, ranging from pest-suppressive to pest-supportive environments, identified for up- and out-scaling.

EBCAs impact data, market receptivity and socio-economic data report, with guidelines for crop biomanagement

Report with parameters and impact data for yield analysis of EBCAs-treated plants, for socio-economic purposes; market receptivity and socio-economic data, including SWOT analysis, guidelines and recommendations for banana and enset crop biomanagement.

Field data for integration of fungi and bacteria-based formulations with susceptible/tolerant germplasm against RKN, BW and PD

Report with main results from field assays on Meloidogyne spp. and other PPN, BW and PD control in Canary Islands, Cuba, Costa Rica and SSA, using susceptible/tolerant gerplasm..

Sampling methods and protocols for EBCAs and qualitative/quantitative epidemiologic data on changes induced in time and under various climatic change effects

Report with data and models for banana production in response to seasonal weather and long-term climate changes, with quantification of the influence of predictor variables on production. For the epidemiological data on banana and enset pests, a molecular toolbox and a model for disease prevalence assessment, risk of spread, and likely impacts on production will be produced, for quantification of the influence of predictors on risk of spread and production impacts.

EBCAs gene expression data in controlled conditions

Report on transcriptomic data on EBCAs-treated banana plants. Sequence data for validation of EBCAs tested. Transcripts identified for most suitable EBCAs in relation to the defense pathways elicited in treated roots or banana tissues, for their use as markers in field trials. Gene expression data of banana susceptible/tolerant response with EBCAs, in nursery conditions, to profile the bananas response before transplant, in relation to treatments with useful microorganisms or EPN.

Kick off meeting report

The kick off meeting will be organized at Canary Island in collaboration with Coplaca. The report will list the participating personnel, the activities developed in the meeting and the work planned for each beneficiary.

Practice abstract (second series)

A total of 10 further practice abstracts related to the achievements of the Project and realized by the second reporting date will be assembled, produced and disseminated. They also include the results of an analysis of the research to be conducted and possible outcomes. The abstracts will be presented as part of a series, based on the knowledge produced by each partner and main practical results, including field data.

Communication, Dissemination and Exploitation Plan

Communication activities will be planned and implemented from the beginning of the Project, based on a comprehensive Communication, Dissemination and Exploitation Plan (CDEP) that will be delivered by month 4 by the Consortium and whose development will proceed from internal periodic reports, including a record of activities related to dissemination and exploitation that have been undertaken and those planned. The CDEP includes the Data Management Plan (DMP). The CDEP will consider the following aspects: the measures to be implemented during the project for dissemination, how data will be exploited and/or shared/made accessible, the ways to involve potential final users and stakeholders and the local regional political context, the activities related to education and training, the final application of results and the measures to be implemented after the end of the Project. The MUSA Management Committee will periodically check and follow the CDEP for identification of transferable knowledge and/or publications and production of other communication tools. The dissemination procedures will have different scales, depending on targets. The CDEP will show how data will be exploited and/or shared/made accessible for verification and use.

Validation SWOT report on bio-formulations parameters for IPM, transfer of methodologies and management of simultaneous pests

Report with SWOT analysis results on the bioformulation efficacy in the field, including the comparative examination of different bioformulations/microorganisms or EPNs, for evaluation of their overall potential in IPM of banana in different regions, and eventual adoption by small holders. The report includes analysis of main characteristics of the bioformulations developed (represented by a number of parameters such as water content, composition in terms of species/isolates, pH, presence of additional substrates and compounds, additives, stabilizers, including packaging and shelf-life), that will be required for successful IPM application. Includes numerical results indicating the efficacy of the EBCAs formulated to control or suppress their target PPN, PD and BW, suitable for use in modelling or other dissemination and communication tasks.

Microbial collections (bacteria, fungi) and other beneficial EBCAs for IPM and selected PGPM/soil microorganisms

A number of collections of microorganisms proceeding from the sampling sites will be maintained at corresponding national partners. Sequence data produced for their identification will be made available on public databases (NCBI, EMBO). In case of species described as new for science a number of isolates will be deposited in public collections (CBS, DSMZ). Most promising isolates of PGPM/soil microorganisms, that will be distributed and used in field assays for PD and PPN management with stakeholders, including selected EBCAs for IPM of RKN and other nematodes based on P. chlamydosporia and/or soil bacteria or fungi and entomopathogenic fungi or antagonists for biocontrol field assays of BW.

Practice abstracts (first series)

A total of 8 practice abstracts related to the achievements of the Project realized by the first reporting date will be assembled, produced and disseminated. They also include the results of an analysis of the reserarch to be conducted and possible outcomes. The abstracts will be presented as part of a series, based on the knowledge produced by each partner and main practical results, including field data.

Plant-derived products and helpers for BW management and bioformulations

Plant-derived products and helpers for BW management. Bioformulations for application on banana and enset. Developed bioformulation prototypes based on EBCAs for application on banana and enset against pathogens and pests.

Metagenomic data on soil microbial consortia effects on EBCAs on susceptible/tolerant banana and enset

Identification of all the bacterial taxonomic units present in the rhizosphere of treated bananas, and of the effects induced by a range of treatments, including EBCAs, chemicals and nematocides.

Data Management Plan

Data Management Plan concerning the dissemination and exploitation activities to be carried out by the Consortium, and those still planned.

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Publications

Genome sequence data from 17 accessions of Ensete ventricosum , a staple food crop for millions in Ethiopia

Author(s): Zerihun Yemataw, Sadik Muzemil, Daniel Ambachew, Leena Tripathi, Kassahun Tesfaye, Alemayheu Chala, Audrey Farbos, Paul O’Neill, Karen Moore, Murray Grant, David J. Studholme
Published in: Data in Brief, Issue 18, 2018, Page(s) 285-293, ISSN 2352-3409
DOI: 10.1016/j.dib.2018.03.026

Molecular Mechanisms of Chitosan Interactions with Fungi and Plants

Author(s): Federico Lopez-Moya, Marta Suarez-Fernandez, Luis Lopez-Llorca
Published in: International Journal of Molecular Sciences, Issue 20/2, 2019, Page(s) 332, ISSN 1422-0067
DOI: 10.3390/ijms20020332

Biological Control Agents Against Fusarium Wilt of Banana

Author(s): Giovanni Bubici, Manoj Kaushal, Maria Isabella Prigigallo, Carmen Gómez-Lama Cabanás, Jesús Mercado-Blanco
Published in: Frontiers in Microbiology, Issue 10, 2019, ISSN 1664-302X
DOI: 10.3389/fmicb.2019.00616

Entomopathogenic nematology in Latin America: A brief history, current research and future prospects

Author(s): Ernesto San-Blas, Raquel Campos-Herrera, Claudia Dolinski, Caio Monteiro, Vanessa Andaló, Luis Garrigós Leite, Mayra G. Rodríguez, Patricia Morales-Montero, Adriana Sáenz-Aponte, Carolina Cedano, Juan Carlos López-Nuñez, Eleodoro Del Valle, Marcelo Doucet, Paola Lax, Patricia D. Navarro, Francisco Báez, Pablo Llumiquinga, Jaime Ruiz-Vega, Abby Guerra-Moreno, S. Patricia Stock
Published in: Journal of Invertebrate Pathology, Issue 165, 2019, Page(s) 22-45, ISSN 0022-2011
DOI: 10.1016/j.jip.2019.03.010

Unlocking the Microbiome Communities of Banana (Musa spp.) under Disease Stressed (Fusarium wilt) and Non-Stressed Conditions

Author(s): Manoj Kaushal, Rony Swennen, George Mahuku
Published in: Microorganisms, Issue 8/3, 2020, Page(s) 443, ISSN 2076-2607
DOI: 10.3390/microorganisms8030443

La introducción de cilindros de vidrio y mallas plásticas en olfatómetro facilita ensayos de comportamiento de nematodos entomopatógenos

Author(s): Giselle Calabuche-Gómez, Roberto Enrique Regalado, Lidia López Perdomo
Published in: Revista de Protección Vegetal, Issue 34 (3), 2019, ISSN 2224-4697

Efecto de factores abióticos sobre la viabilidad e infectividad de Heterorhabditis amazonensis Andaló et al. Cepa HC1

Author(s): Giselle Calabuche-Gómez, Roberto Enrique Regalado, Dayris García Perera, Ileana Miranda Cabrera, Dulce María Soler, Mayra G. Rodríguez Hernández
Published in: Revista de Protección Vegetal, Issue 34 (3), 2019, ISSN 2224-4697

Susceptibilidad de adultos de Cosmopolites sordidus (Germar) a Heterorhabditis amazonensis Andaló et al. Cepa HC1

Author(s): Dairys García-Perera, Roberto Enrique, Lidia López, Daine Hernández-Ochandía, Ileana Miranda, Giselle Calabuche-Gómez, Oriela Pino, Lester Pupiro, Mayra G. Rodríguez
Published in: Revista de Protección Vegetal, Issue 34 (3), 2019, ISSN 2224-4697

Commelina diffusa Burm. F., nuevo hospedante de Meloidogyne sp. en Cuba

Author(s): Vániert Ventura-Chávez, Daine Hernández-Ochandía, Belkis Peteira Delgado-Oramas, Mayra G. Rodríguez Hernández
Published in: Revista de Protección Vegetal, Issue 34 (3), 2019, ISSN 2224-4697

Un approccio ecosostenibile per la difesa del banano

Author(s): Aurelio Ciancio, Fabrizio Cillo
Published in: Sapere, Issue 84, 6, 2018, 2018, Page(s) 10-15, ISSN 0036-4681
DOI: 10.12919/sapere.2018.06.1

Microbiome-based services for sustainable nematode management

Author(s): Ciancio A., Laura C. Rosso, Mariantonietta Colagiero, Isabella Pentimone
Published in: NEMATROPICA - 50th Annual Meeting/Organization of Nematologists of Tropical America, Issue 48 (2), 2018, Page(s) Abs. 6, ISSN 2220-5608

The banana root endosphere microbiota is an important reservoir of potential biocontrol agents against Fusarium oxysporum f. sp. cubense

Author(s): Gómez-Lama Cabanás, Carmen; Valverde-Corredor, Antonio; López Cepero, J.; Cardoni, Martina; Fernández-González, Antonio José; Fernández-López, Manuel; Mercado-Blanco, Jesús
Published in: Congress of Microbiology and Biotechnology (Microbiotec 19), Coimbra 5- 7 dec. 2019, Issue 1, 2019
DOI: 10.13039/501100000780

A metagenomic study of banana nematode antagonists in Canary Islands

Author(s): A. Ciancio, M. Colagiero, L. C. Rosso, I. Pentimone, J. López Cepero
Published in: NEMATROPICA - 51st Annual Meeting of the Organization of Nematologists of Tropical America, Issue 49 (2), 2019, Page(s) Abst. 7