Periodic Reporting for period 1 - NeuronAgeScreen (A Drug Discovery and Target Identification Screening Platform for Age-Associated Neurodegenerative Disorders)
Reporting period: 2017-05-01 to 2018-10-31
Battling human neurodegenerative pathologies, and their pervasive societal impact, is a global multi-billion Euro enterprise. Ageing is universally associated with marked decrease of neuronal function and higher susceptibility to neurodegeneration. In human populations, this is manifested as an ever-increasing prevalence of devastating neurodegenerative conditions, including Alzheimer’s and Parkinson’s disease, stroke, several ataxias, and other types of dementia. Development of therapeutic interventions against such maladies is becoming a pressing priority. Drug discovery and drug target identification are two intimately linked facets of intervention strategies aimed at effectively combating human disorders. Genes linked to human diseases often function in evolutionary conserved pathways, readily dissected in simple model organisms. Such organisms provide attractive platforms for devising and streamlining efficient drug discovery and target identification methodologies. During the course of the ERC project NeuronAge, we developed a convenient and versatile platform for high-throughput chemical compound screening based on the nematode C. elegans. This innovative platform uniquely combines state-of-the-art microfluidics technologies for imaging and manipulation of neurons in vivo, with the experimental prowess of C. elegans, a highly malleable genetic model, which offers a precisely defined nervous system, two features that are not available in any other organism. With the support provided by the ERC NeuronAgeScreen Proof-of-Concept project, we have optimized and streamlined the high-throughput compound screening system, which is now at a pre-demonstration stage, as initially envisioned. Moreover we have established its dependability for drug target identification and drug discovery, by thorough assessment using animal models of human neurodegenerative disorders.