Synthetic Cellularity via Protocell Design and Chemical Construction

Project Information

PCELLS

Grant agreement ID: 740235

DOI 10.3030/740235

Project closed

EC signature date 10 April 2017

Start date 1 July 2017

End date 31 December 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 499 238,00

EU contribution € 2 499 238,00

Coordinated by

UNIVERSITY OF BRISTOL
United Kingdom

Publications

Peer reviewed articles (15)

Photosynthetic Hydrogen Production by Droplet-based Microbial Micro-reactors under Aerobic Conditions.

Author(s): Xu Z, Wang S, Zhao C, Li S, Liu X, Wang L, Li M, Huang X and Mann S.
Published in: Nature Communications, Issue 11, 2020, Page(s) 5985, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-19823-5

Hydrogel-immobilized Coacervate Droplets as Modular Micro-reactor Assemblies.

Author(s): Liu J, Tian L, Qiao Y, Zhou S, Patil A J, Wang K, Li M, Mann S.
Published in: Angewandte Chemie International Edition, Issue 59, 2020, Page(s) 6853-6859, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201916481

Giant Coacervate Vesicles as an Integrated Approach to Cytomimetic Modelling.

Author(s): Zhang Y, Chen Y, Yang X, Li M, Liu S, Wang K, Liu J and Mann S.
Published in: J. Am. Chem. Soc., Issue 143, 2021, Page(s) 2866−2874, ISSN 1520-5126
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c12494

Enzyme-mediated Nitric Oxide Production in Vasoactive Erythrocyte Membrane-enclosed Coacervate Protocells.

Author(s): Liu S, Zhang Y, Li M, Xiong L, Yang X, He X, Wang K, Liu J and Mann S
Published in: Nature Chemistry, Issue 12, 2020, Page(s) 1165-1173, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-020-00585-y

Chemical Mediated Translocation in Protocell-based Micro-actuators.

Author(s): Ning Gao, Mei Li, Liangfei Tian, Avinash. J. Patil, P B V V S Pavan Kumar, Stephen Mann
Published in: Nature Chemistry, Issue 13, 2021, Page(s) 868–879, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-021-00728-9

Preparation of Swellable Hydrogel-Containing Colloidosomes from Aqueous Two-Phase Pickering Emulsion Droplets

Author(s): Jean-Paul Douliez, Nicolas Martin, Thomas Beneyton, Jean-Charles Eloi, Jean-Paul Chapel, Laurence Navailles, Jean-Christophe Baret, Stephen Mann, Laure Béven
Published in: Angewandte Chemie International Edition, Issue 57/26, 2018, Page(s) 7780-7784, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201802929

Chloroplast-containing coacervate micro-droplets as a step towards photosynthetically active membrane-free protocells

Author(s): B. V. V. S. Pavan Kumar, James Fothergill, Joshua Bretherton, Liangfei Tian, Avinash J. Patil, Sean A. Davis, Stephen Mann
Published in: Chemical Communications, Issue 54/29, 2018, Page(s) 3594-3597, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc01129j

Antagonistic chemical coupling in self-reconfigurable host–guest protocells

Author(s): Nicolas Martin, Jean-Paul Douliez, Yan Qiao, Richard Booth, Mei Li, Stephen Mann
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06087-3

Enzyme-powered motility in buoyant organoclay/DNA protocells

Author(s): B. V. V. S. Pavan Kumar, Avinash J. Patil, Stephen Mann
Published in: Nature Chemistry, Issue 10/11, 2018, Page(s) 1154-1163, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-018-0119-3

Modulation of Higher-order Behaviour in Model Protocell Communities by Artificial Phagocytosis

Author(s): Laura Rodríguez-Arco, B. V. V. S. Pavan Kumar, Mei Li, Avinash J. Patil, Stephen Mann
Published in: Angewandte Chemie International Edition, Issue 58/19, 2019, Page(s) 6333-6337, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201901469

DNA-based communication in populations of synthetic protocells

Author(s): Alex Joesaar, Shuo Yang, Bas Bögels, Ardjan van der Linden, Pascal Pieters, B. V. V. S. Pavan Kumar, Neil Dalchau, Andrew Phillips, Stephen Mann, Tom F. A. de Greef
Published in: Nature Nanotechnology, Issue 14/4, 2019, Page(s) 369-378, ISSN 1748-3387
Publisher: Nature Publishing Group
DOI: 10.1038/s41565-019-0399-9

Photoswitchable Phase Separation and Oligonucleotide Trafficking in DNA Coacervate Microdroplets

Author(s): Nicolas Martin, Liangfei Tian, Dan Spencer, Angélique Coutable-Pennarun, J. L. Ross Anderson, Stephen Mann
Published in: Angewandte Chemie International Edition, Issue 58/41, 2019, Page(s) 14594-14598, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201909228

Catalytic processing in ruthenium-based polyoxometalate coacervate protocells

Author(s): Pierangelo Gobbo, Liangfei Tian, B. V. V. S Pavan Kumar, Samuel Turvey, Mattia Cattelan, Avinash J. Patil, Mauro Carraro, Marcella Bonchio, Stephen Mann
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-13759-1

Spontaneous Membrane-less Multi-compartmentalisation via Aqueous Two-phase Separation in Complex Coacervate Micro-droplets.

Author(s): Moreau N G, Martin N, Gobbo P, Tang T-Y D and Mann S.
Published in: Chemical Communications, Issue 56, 2020, Page(s) 12717-12720, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc05399f

A Novel Acid-degradable PEG Crosslinker for the Fabrication of pH-responsive Soft Materials.

Author(s): Myrgorodska I, Jenkinson-Finch M, Moreno Tortolero R, Mann S and Gobbo P.
Published in: Macromol. Rapid Commun., Issue 494, 2021, Page(s) 2100102, ISSN 1521-3927
Publisher: Wiley
DOI: 10.1002/marc.202100102

Conference proceedings (1)

Light-activated Signaling in DNA-encoded Sender-receiver Architectures.

Author(s): Yang S, Pieters P A, Joesaar A, Bögels B W A, Brouwers R, Myrgorodska I, Mann S and de Greef T F A.
Published in: ACS Nano, Issue 14, 2020, Page(s) 15992-16002
Publisher: American Chemical Society
DOI: 10.1021/acsnano.0c07537