Multidimensional CRISPR/Cas mediated engineering of plant breeding

Project Information

CRISBREED

Grant agreement ID: 741306

DOI

10.3030/741306

Project closed

EC signature date 28 June 2017

Start date 1 October 2017

End date 30 September 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 499 981,27

EU contribution

€ 2 499 981,00

Coordinated by

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (21)

CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana

Author(s): Rönspies, Michelle; Schindele, Patrick; Wetzel, Rebecca; Puchta, Holger
Published in: Nature Protocols, Issue 1, 2022, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-022-00686-7

Updates on gene editing and its applications

Author(s): Puchta, Holger; Jiang, Jiming; Wang, Kan; Zhao, Yunde
Published in: Plant Physiology, Issue 1, 2022, Page(s) 1725–1730, ISSN 1532-2548
Publisher: American Society of Plant Biologists
DOI: 10.1093/plphys/kiac032

CRISPR–Cas-mediated chromosome engineering for crop improvement and synthetic biology

Author(s): Rönspies, Michelle; Dorn, Annika; Schindele, Patrick; Puchta, Holger
Published in: Nature Plants, Issue 1, 2021, Page(s) 566–573, ISSN 2055-0278
Publisher: Nature Research
DOI: 10.1038/s41477-021-00910-4

CRISPR/Cas-mediated chromosome engineering: opening up a new avenue for plant breeding

Author(s): Rönspies, Michelle; Schindele, Patrick; Puchta, Holger; Lunn, John
Published in: The journal of experimental botany, Issue 6, 2021, Page(s) 177–183, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/eraa463

The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A

Author(s): Sarah Röhrig, Annika Dorn, Janina Enderle, Angelina Schindele, Natalie J. Herrmann, Alexander Knoll, Holger Puchta
Published in: New Phytologist, Issue 218/4, 2018, Page(s) 1478-1490, ISSN 0028-646X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/nph.15109

The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates

Author(s): Annika Dorn, Sarah Röhrig, Kristin Papp, Susan Schröpfer, Frank Hartung, Alexander Knoll, Holger Puchta
Published in: PLOS Genetics, Issue 14/9, 2018, Page(s) e1007674, ISSN 1553-7404
Publisher: Public Library of Science
DOI: 10.1371/journal.pgen.1007674

Efficient induction of heritable inversions in plant genomes using the CRISPR /Cas system

Author(s): Carla Schmidt, Michael Pacher, Holger Puchta
Published in: The Plant Journal, 2019, ISSN 0960-7412
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/tpj.14322

The protease WSS1A, the endonuclease MUS81 and the phosphodiesterase TDP1 are involved in independent pathways of DNA-protein crosslink repair in plants

Author(s): Janina Enderle, Annika Dorn, Natalja Beying, Oliver Trapp, Holger Puchta
Published in: The Plant Cell, 2019, Page(s) tpc.00824.2018, ISSN 1040-4651
Publisher: American Society of Plant Biologists
DOI: 10.1105/tpc.18.00824

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

Author(s): Patrick Schindele, Felix Wolter, Holger Puchta
Published in: FEBS Letters, Issue 592/12, 2018, Page(s) 1954-1967, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13073

The CRISPR/Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists

Author(s): Felix Wolter, Holger Puchta
Published in: The Plant Journal, Issue 94/5, 2018, Page(s) 767-775, ISSN 0960-7412
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/tpj.13899

CRISPR/Cas brings plant biology and breeding into the fast lane

Author(s): Angelina Schindele, Annika Dorn, Holger Puchta
Published in: Current Opinion in Biotechnology, Issue 61, 2020, Page(s) 7-14, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2019.08.006

DNA Helicases as Safekeepers of Genome Stability in Plants

Author(s): Annika Dorn, Holger Puchta
Published in: Genes, Issue 10/12, 2019, Page(s) 1028, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10121028

From gene editing to genome engineering: restructuring plant chromosomes via CRISPR/Cas

Author(s): Carla Schmidt, Patrick Schindele, Holger Puchta
Published in: aBIOTECH, Issue 1/1, 2020, Page(s) 21-31, ISSN 2096-6326
Publisher: Springer
DOI: 10.1007/s42994-019-00002-0

Plant breeding at the speed of light: the power of CRISPR/Cas to generate directed genetic diversity at multiple sites

Author(s): Felix Wolter, Patrick Schindele, Holger Puchta
Published in: BMC Plant Biology, Issue 19/1, 2019, ISSN 1471-2229
Publisher: BioMed Central
DOI: 10.1186/s12870-019-1775-1

An Arabidopsis FANCJ helicase homologue is required for DNA crosslink repair and rDNA repeat stability

Author(s): Annika Dorn, Laura Feller, Dominique Castri, Sarah Röhrig, Janina Enderle, Natalie J. Herrmann, Astrid Block-Schmidt, Oliver Trapp, Laura Köhler, Holger Puchta
Published in: PLOS Genetics, Issue 15/5, 2019, Page(s) e1008174, ISSN 1553-7404
Publisher: Public Libary of Science
DOI: 10.1371/journal.pgen.1008174

CRISPR–Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis

Author(s): Natalja Beying, Carla Schmidt, Michael Pacher, Andreas Houben, Holger Puchta
Published in: Nature Plants, Issue 6/6, 2020, Page(s) 638-645, ISSN 2055-0278
Publisher: Nature Research
DOI: 10.1038/s41477-020-0663-x

Sophisticated CRISPR/Cas tools for fine-tuning plant performance

Author(s): Capdeville, Niklas; Merker, Laura; Schindele, Patrick; Puchta, Holger
Published in: Journal of plant physiology, Issue 1, 2021, Page(s) 153332, ISSN 0176-1617
Publisher: Elsevier BV
DOI: 10.1016/j.jplph.2020.153332

Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering

Author(s): Michelle Rönspies; Carla Schmidt; Michal Lieberman-Lazarovich; Patrick Schindele; Andreas Houben; Holger Puchta
Published in: Nature plants, Issue 1, 2022, ISSN 2055-0278
Publisher: Nature Research
DOI: 10.1038/s41477-022-01238-3

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Author(s): Carla Schmidt, Paul Fransz, Michelle Rönspies, Steven Dreissig, Jörg Fuchs, Stefan Heckmann, Andreas Houben, Holger Puchta
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-18277-z

Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering

Author(s): Gehrke, Fabienne; Schindele, Angelina; Puchta, Holger
Published in: Plant physiology, Issue 2, 2021, ISSN 1532-2548
Publisher: American Society of Plant Biologists
DOI: 10.1093/plphys/kiab572

Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

Author(s): Schindele, Angelina; Gehrke, Fabienne; Schmidt, Carla; Röhrig, Sarah; Dorn, Annika; Puchta, Holger
Published in: Nature Communications, Issue 2, 2022, Page(s) 1502, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-29130-w

Book chapters (1)

Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants

Author(s): Annika Dorn, Holger Puchta
Published in: Plant Meiosis - Methods and Protocols, Issue 2061, 2020, Page(s) 359-366, ISBN 978-1-4939-9817-3
Publisher: Springer New York
DOI: 10.1007/978-1-4939-9818-0_25