Single-cell temporal tracking of epigenetic DNA marks

Project Information

EpiTrack

Grant agreement ID: 742654

DOI

10.3030/742654

Project closed

EC signature date 21 April 2017

Start date 1 September 2017

End date 28 February 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 499 875,00

EU contribution

€ 2 499 875,00

Coordinated by

VILNIAUS UNIVERSITETAS
Lithuania

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (13)

Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs

Author(s): Milda Mickutė, Kotryna Kvederavičiūtė, Aleksandr Osipenko, Raminta Mineikaitė, Saulius Klimašauskas, Giedrius Vilkaitis
Published in: BMC Biology, Issue 19/1, 2021, Page(s) 129, ISSN 1741-7007
Publisher: BioMed Central
DOI: 10.1186/s12915-021-01053-w

Enzymatic hydroxylation and excision of extended 5-methylcytosine analogues

Author(s): Miglė Tomkuvienė, Diana Ikasalaitė, Anton Slyvka, Audronė Rukšėnaitė, Mirunalini Ravichandran, Tomasz P. Jurkowski, Matthias Bochtler, Saulius Klimašauskas
Published in: Journal of Molecular Biology, Issue 423, 2020, Page(s) 6157-6167, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2020.10.011

Engineered Methionine Adenosyltransferase Cascades for Metabolic Labeling of Individual DNA Methylomes in Live Cells

Author(s): Liepa Gasiulė, Vaidotas Stankevičius, Kotryna Kvederavičiu̅tė, Jonas Mindaugas Rimšelis, Vaidas Klimkevičius, Gražina Petraitytė, Audronė Rukšėnaitė, Viktoras Masevičius, Saulius Klimašauskas
Published in: Journal of the American Chemical Society, Issue 146, 2024, Page(s) 18722-18729, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c06529

Selective chemical tracking of Dnmt1 catalytic activity in live cells.

Author(s): V. Stankevičius, P. Gibas, B. Masiulionytė, L. Gasiulė, V. Masevičius, S. Klimašauskas, G. Vilkaitis
Published in: Molecular Cell, Issue 82/5, 2022, Page(s) 1053-1065.e8, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2022.02.008

Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg Protein

Author(s): Almaz Nigatu Tesfahun, Marina Alexeeva, Miglė Tomkuvienė, Aysha Arshad, Prashanna Guragain, Arne Klungland, Saulius Klimašauskas, Peter Ruoff, Svein Bjelland
Published in: Frontiers in Microbiology, Issue 12, 2021, Page(s) 608839, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.608839

Distribution and regulatory roles of oxidized 5-methylcytosines in DNA and RNA of the Basidiomycete fungi Laccaria bicolor and Coprinopsis cinerea.

Author(s): J. Ličytė, K. Kvederavičiūtė, A. Rukšėnaitė, E. Godliauskaitė, P. Gibas, V. Tomkutė, G. Petraitytė, V. Masevičius, S. Klimašauskas, E. Kriukienė
Published in: Open Biology, Issue 12/3, 2022, Page(s) 210302, ISSN 2046-2441
Publisher: Royal Society Publishing
DOI: 10.1098/rsob.210302

Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA

Author(s): Miglė Tomkuvienė, Milda Mickutė, Giedrius Vilkaitis, Saulius Klimašauskas
Published in: Current Opinion in Biotechnology, Issue 55, 2019, Page(s) 114-123, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2018.09.008

Animal Hen1 2′-O-methyltransferases as tools for 3′-terminal functionalization and labelling of single-stranded RNAs

Author(s): Milda Mickutė, Milda Nainytė, Lina Vasiliauskaitė, Alexandra Plotnikova, Viktoras Masevičius, Saulius Klimašauskas, Giedrius Vilkaitis
Published in: Nucleic Acids Research, Issue 46/17, 2018, Page(s) e104-e104, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gky514

An Adversarial DNA N6-Methyladenine-Sensor Network Preserves Polycomb Silencing

Author(s): Soo-Mi Kweon, Yibu Chen, Eugene Moon, Kotryna Kvederaviciutė, Saulius Klimasauskas, Douglas E. Feldman
Published in: Molecular Cell, Issue 74, 2019, Page(s) 1138-1147.e6, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2019.03.018

Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing

Author(s): Povilas Gibas, Milda Narmontė, Zdislav Staševskij, Juozas Gordevičius, Saulius Klimašauskas, Edita Kriukienė
Published in: PLOS Biology, Issue 18, 2020, Page(s) e3000684, ISSN 1544-9173
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.3000684

Enhanced nucleosome assembly at CpG sites containing an extended 5-methylcytosine analogue

Author(s): Miglė Tomkuvienė, Markus Meier, Diana Ikasalaitė, Julia Wildenauer, Visvaldas Kairys, Saulius Klimašauskas, Laura Manelytė
Published in: Nucleic Acids Research, Issue 50/11, 2022, Page(s) 6549–6561, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac444

FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine.

Author(s): María José Peña-Gómez, Paula Moreno-Gordillo, Milda Narmontė, Clara B. García-Calderón, Audronė Rukšėnaitė, Saulius Klimašauskas, Iván V. Rosado
Published in: Cell Death & Disease, Issue 13/5, 2022, Page(s) 503, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-022-04952-0

One-pot trimodal mapping of unmethylated, hydroxymethylated and open chromatin sites unveils distinctive 5hmC roles at dynamic chromatin loci.

Author(s): K. Skardžiūtė, K. Kvederavičiūtė, I. Pečiulienė, M. Narmontė, P. Gibas, J. Ličytė, S. Klimašauskas, E. Kriukienė
Published in: Cell Chemical Biology, 2024, Page(s) in press, ISSN 2451-9456
Publisher: Cell Press
DOI: 10.1016/j.chembiol.2023.12.003

Non-peer reviewed articles (1)

Meet the author: Vaidotas Stankevičius, Giedrius Vilkaitis, and Saulius Klimašauskas

Author(s): Vaidotas Stankevičius, Giedrius Vilkaitis, and Saulius Klimašauskas
Published in: Molecular Cell, Issue 82/5, 2022, Page(s) 879-881, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2022.02.019

Book chapters (1)

DNA Labeling Using DNA Methyltransferases

Author(s): Miglė Tomkuvienė, Edita Kriukienė, Saulius Klimašauskas
Published in: Advances in Experimental Medicine and Biology, Issue 1389, 2022, Page(s) 535–562, ISBN 978-3-031-11454-0
Publisher: Springer
DOI: 10.1007/978-3-031-11454-0_19