Objective The Nanoparticle Biointerface Mapping project builds on the extensive bionano interactions research in the group of Professor Kenneth Dawson at University College Dublin. In the NanoBioMap project I propose a cutting-edge interdisciplinary approach to develop a versatile tool allowing me to obtain detailed 3D maps of biomolecules at the surface of nanoparticles in complex biological media and study their importance for cell interactions. I will build on my extensive knowledge of nanomaterial science to develop this 3D mapping tool combining a tailor-made library of antibody-tagged quantum dots with electron microscopy 3D image reconstruction and X-ray elemental mapping. The resulting versatile method has multiple uses far beyond analyzing protein coronas. I envision applications of this state-of-the-art mapping tool in areas such as vaccine development, enzymatic catalysis on supported particles, cancer treatment and tracking of pollutants in water flows. The proposed project has 4 objective which will be reached over 24 months, within 13 work packages, including training-through-research and acquisition of transferable and complementary skills, contributing to the advancement of my career beyond this project, as a future independent researcher. The main objective is developing a tool to allow 3D mapping of protein epitopes at the surface of nanosized objects, with applications far beyond my test systems. The other objectives involve testing my tool to map epitopes in the protein corona at nanoparticles and study their interaction with cell receptors, focusing on spatial distribution of specific receptors. The proposal builds on my current extensive knowledge of nanomaterial synthesis, surface modification and characterization in complex environments adding a cell biology dimension, thus expanding my knowledge and career prospects. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencescell biologynatural sciencesphysical sciencesopticsmicroscopyelectron microscopynatural scienceschemical sciencescatalysisengineering and technologynanotechnologynano-materials Keywords protein epitope mapping interdisciplinary quantum dots antibodies protein corona catalysis nanomedicine biomolecules Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2016 - Individual Fellowships Call for proposal H2020-MSCA-IF-2016 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN Net EU contribution € 175 866,00 Address Belfield 4 Dublin Ireland See on map Region Ireland Eastern and Midland Dublin Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
