Objetivo Bacteriophages (phages) drive the evolution of bacteria by antagonistic coevolution and by promoting transfer of genetic material between bacteria. Phages are intimately involved in a range of biological processes from carbon cycling to the maintenance of human health. Despite their global importance the coevolution between natural phage communities and their bacterial hosts remains poorly understood. This project seeks to understand the mechanism behind a Phage-Induced High-Cell-Density (PIHCD) phenotype using a droplet-based microfluidic device termed the Evolution Machine, developed at the proposed institution (ESPCI Paris). Work Package 1 (WP1) will use the Evolution Machine to determine and characterize the phage(s) responsible for the PIHCD phenotype through coinfection with their Pseudomonas fluorescens host. WP2 will determine the mechanistic basis of the PIHCD phenotype using transcriptomics and genetic manipulation. Finally, in WP3 the long term coevolution between phage(s) and host will be investigated using the droplet-level selection capabilities of the Evolution Machine combined with comparative genomic analysis. Through this fellowship ESPCI Paris will gain from my expertise in molecular biology and bioinformatics while I will develop new skills in microbiology (phage-host coevolution and genetic manipulation) and microfluidic technology (Evolution Machine). Ámbito científico ciencias naturalesciencias físicasmecánica clásicamecánica de fluidosmicrofluidicsciencias naturalesciencias biológicasmicrobiologíabacteriologíaciencias naturalesciencias biológicasmicrobiologíavirologíaciencias naturalesciencias biológicasbiología evolutivaciencias naturalesciencias físicasastronomíaciencias planetariasplanetas Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2016 - Individual Fellowships Convocatoria de propuestas H2020-MSCA-IF-2016 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-ST - Standard EF Coordinador ECOLE SUPERIEURE DE PHYSIQUE ET DECHIMIE INDUSTRIELLES DE LA VILLE DEPARIS Aportación neta de la UEn € 173 076,00 Dirección Rue vauquelin 10 75231 Paris Francia Ver en el mapa Región Ile-de-France Ile-de-France Paris Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Otras fuentes de financiación € 0,00
