Objective
Non DNA-sequence based inheritance has been observed in many organisms from microbes to man and likely impacts public health. The mechanisms of non DNA-sequence based inheritance remain largely unknown. However, some examples of non DNA-sequence based inheritance in mammals, plants and invertebrates are linked to the control of selfish, transposable elements in the genome. Here we propose to discover the mechanistic basis of multi-generational non DNA-sequence based inheritance, also referred to as transgenerational epigenetic inheritance (TEI), using the laboratory animal model Caenorhabditis elegans. Specifically, our goal is to identify chromatin and non-coding RNA “epigenetic” marks that transfer information from generation to generation in C. elegans. C. elegans is a great model for this work as it has a generation time of only three days, has a powerful genetics toolkit and its chromatin and RNA pathways are largely conserved in humans. Towards this goal we have established a novel assay we named piRNA-related insertional chromatin immunoprecipitation (piChIP). Using this piChIP system, our specific aims are:
Aim-1: Identification of novel non-coding RNAs in the germline nuclear RNAi pathway
- Method: RNA-seq
Aim-2: Identification of novel proteins and histone PTMs in the germline nuclear RNAi pathway
- Method: SILAC proteomics and Mass Spectrometry
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health scienceshealth sciencespublic health
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- natural sciencesbiological sciencesgeneticsRNA
- natural scienceschemical sciencesanalytical chemistrymass spectrometry
- natural sciencesbiological scienceszoologyinvertebrate zoology
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Programme(s)
Funding Scheme
MSCA-IF-EF-ST - Standard EFCoordinator
CB2 1TN Cambridge
United Kingdom