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Origins and evolution of programmed cell death machinery during the emergence of animal multicellularity

Objective

Programmed cell death (PCD) is an essential function in multicellular organisms; it regulates normal development, responses to cell damage, and cell turnover. Accordingly, the PCD machinery present in the unicellular ancestors of animals was likely critical to the emergence of the complex multicellularity found in extant animals. However, we lack a full understanding of the PCD repertoire in the unicellular relatives of animals, and whether that ancestral repertoire underwent significant changes at the onset of Metazoa

To fill this gap in our understanding we will perform a comparative genomics approach, combined with in vivo experiments, to examine the distribution and evolution of programmed cell death (PCD) proteins in the unicellular relatives of animals. This will identify which components of the PCD machinery were already present in the unicellular ancestor of animals, and the functional and regulatory changes that they might have undergone, and which led to the emergence of apoptosis in animals. Of particular interest is the subtype of PCD known as apoptosis, as the most characteristic apoptosis proteins are thought to be animal-specific.

To investigate whether functional modifications occurred in the PCD repertoire at the origin of animals, we will perform in vivo experiments in two unicellular relatives of animals, Capsaspora and Creolimax, for which the host lab has developed transformation protocols. We will overexpress, and if possible silence, candidate pro- and anti-apoptotic proteins in those taxa to test whether they induce or protect against cell death. We will also examine protein interactions of these proteins during a proposed secondment. This project will provide the first comprehensive reconstruction of programmed cell death machinery across the group comprising animals and their unicellular relatives. The findings will be of interest not only to evolutionary biologists, but also to microbiologists, and to cell and developmental biologists.

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2016

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 158 121,60
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 158 121,60
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