Periodic Reporting for period 1 - LACTOPOC (A Low-cost disposAble eleCTrochemical biOsensor for Point Of Care (POC) hypolactasia diagnostic testing)
Période du rapport: 2017-05-02 au 2019-05-01
The new strategy was subsequently applied for the determination of D-xylose in plasma samples. The plastic-based D-xylose biosensor prototype was tried with spiked plasma samples, showing very promising results: 80% of the tested samples exhibited %recoveries within 85-115% range. This showed the high accuracy and reproducibility of the new system. Further adjustments to test whole blood samples were also tried. Regarding paper substrate, a fully operative D-xylose biosensor was achieved by combining previous advances in plastic-based biosensors and paper-based electrodes. Preliminary analytical data in buffer were shown to be very promising, although further optimization for biological sample testing was required. At the final stage of the project, the analytical and clinical correlation of Osaxyl device (system for D-xylose determination in urea) was performed. The ER participated in the planning and design of the validation protocols. Also, she collaborated in the procedure to achieve CE marking and ISO 13485 certification.
Regarding the biosensor platforms, LACTOPOC project supported the development of a paper-based electrochemical biosensor capable of the quantitative determination of analytes at low concentrations. Although this area is still at an early stage, the advances and optimization work showed in the project represent a thrust toward the consolidation of paper substrate as an alternative to plastic in the market of healthcare biosensors.