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Quantitative super-resolution optical fluctuation and phase microscopy for structural and functional imaging of Parkinson's disease

Objective

The changing demographic is one of the greatest challenges in Europe. With an expected 25% of the population over 65 by 2030, the incidence of neurodegenerative age-linked diseases like Alzheimer’s and Parkinson’s is likely to increase. They severely impact quality of life and to date, therapies can only treat the symptoms. Oligomerization and aggregation of the protein α-synuclein is a hallmark of Parkinson’s disease. Still, the underlying molecular mechanism of cellular dysfunction due to toxic forms of this protein remains unclear. Most studies aiming to uncover α-synuclein induced neurodegeneration in vivo are using indirect biochemical detection methods. Bridging neurobiology and life science with advanced microscopy and image analysis allows the direct monitoring of Parkinson compromised primary hippocampal neurons. I will combine multi-color 3D super-resolution optical fluctuation imaging with quantitative phase tomography in a multi-plane microscope, to allow structural and fast functional imaging. This innovative imaging concept is used (1) to elucidate α-synuclein aggregation at the cell membrane and in the cytosol and (2) to uncover the induced cytoskeleton alterations. Ultimately, molecule-specific readout will be complemented by (3) label-free, quantitative phase imaging of membrane dynamics and overall cell morphology at up to 100Hz. I am a biophysicist with a specialization in single molecule spectroscopy and my previous work on photon statistics has led to several high quality publications. This fellowship expands my quantitative microscopy expertise to 3D imaging while enlarging my biologocal scope to neurodegenerative diseases. I will extend my competences by managing the project, following complementary skills courses, and organizing public outreach activities. While performing interdisciplinary research on neurodegenerative disease, I will develop an independent research profile to further enhance the European research landscape.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2016

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Coordinator

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 419,60
Address
BATIMENT CE 3316 STATION 1
1015 LAUSANNE
Switzerland

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Region
Schweiz/Suisse/Svizzera Région lémanique Vaud
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 175 419,60
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