A droplet microfluidic system for continuous in vivo evolution.

Project Information

DropContEvo

Grant agreement ID: 750772

DOI

10.3030/750772

Project closed

EC signature date 9 March 2017

Start date 1 January 2018

End date 31 December 2019

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 183 454,80

EU contribution

€ 183 454,80

Coordinated by

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (7)

High-throughput total RNA sequencing in single cells using VASA-seq.

Author(s): Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder & Alexander van Oudenaarden.
Published in: Nature Biotechnology, Issue 10870156, 2022, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-022-01361-8

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Author(s): Elliot J. Medcalf; Maximilian Gantz; Tomasz S. Kaminski; Florian Hollfelder
Published in: Analytical Chemistry, Issue Vol. 95, Issue 10, 2023, Page(s) 4597–4604, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.2c04144

Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

Author(s): Miriam Klaus, Paul Jannis Zurek, Tomasz S. Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Published in: ChemBioChem, Issue 14394227, 2021, Page(s) 3292-3299, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100322

Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets

Author(s): Simon Ladeveze; Paul J. Zurek; Tomasz S. Kaminski; Stephane Emond; Florian Hollfelder
Published in: ACS Catalysis, Issue Vol. 13, Issue 15, 2023, Page(s) 10232–10243, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.3c01609

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

Author(s): Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte E. Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Published in: Nature Communications, Issue Vol. 14, 2023, Page(s) 4788, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-40322-w

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase

Author(s): David Schnettler Fernández; Oskar James Klein; Tomasz S. Kaminski; Pierre-Yves Colin; Florian Hollfelder
Published in: Journal of American Chemical Society, Issue Volume145, Issue 2, 2023, Page(s) 1083-1096, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c10673

Vector redesign and in-droplet cell-growth improves enrichment and recovery in live Escherichia coli.

Author(s): Bernard D. G. Eenink, Tomasz S. Kaminski, Erich Bornberg-Bauer, Joachim Jose, Florian Hollfelder, Bert van Loo.
Published in: Microbial Biotechnology, Issue 17517907, 2022, ISSN 1751-7907
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1751-7915.14144