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A LAB-ON-A-CHIP DEVICE FOR PURE CIRCULATING TUMOR CELL ISOLATION FROM WHOLE BLOOD FOR CANCER THERAPY

Periodic Reporting for period 1 - pureCTC (A LAB-ON-A-CHIP DEVICE FOR PURE CIRCULATING TUMOR CELL ISOLATION FROM WHOLE BLOOD FOR CANCER THERAPY)

Reporting period: 2017-08-01 to 2019-07-31

The overall objective of pureCTC is to realize a comprehensive lab-on-a-chip platform that redefines the state-of-the-art technology to address all the requirements for fast, efficient and reliable CTC isolation/purification from whole blood.
CTCs are rare cells that originate from tumors, and which travel in the human circulatory system to eventually spread the disease to other organs. Consequently, analysis of CTCs is crucial for revealing the mechanisms of cancer and developing new treatments. However, only 1-10 CTCs are present per mL of blood against billions of peripheral blood cells. The extreme rarity and inherent heterogeneity of CTCs implies a single-cell analysis strategy. Therefore, a highly selective, efficient and reliable system yet providing high throughput is mandatory. Cell labeling based strategies are inefficient for CTC analysis, since CTCs express biomarkers with high heterogeneity. Label-free methods based on size lack the ability to distinguish CTCs from leukocytes, and methods based on electrical properties fail to provide sufficient enrichment or throughput levels.
To address these challenges, pureCTC proposes the development a microfluidic platform for successive enrichment, identification, and isolation of CTCs at the single-cell level, where a blood sample taken from a patient is directly injected and utilized without any pre-treatment. Namely, the platform is composed of 3 modules that work in tandem, each of them having innovative aspects: enrichment via 2D hydrodynamic-dielectrophoretic separation, identification via synchronous electro-optical detection, and isolation via a dielectrophoretic valve.
The proposed research will undoubtedly contribute to the knowledge-based economy and European society, specifically on cancer research by providing an instrumental platform for diagnosis, monitoring and treatment of diseases with unprecedented selectivity, and enabling the advancement of personalized treatments.
During pureCTC, a highly sensitive microscale flow cytometer was realized. It was possible to discriminate tumor cells from the rest of the blood cells. The developed technology showed great potential as an abnormal detection device, as well as an hematology analyzer. The IPR on the core technology, where pureCTC brought the novelty was protected and the results will be published and communicated in a year period.
pureCTC started with an ambitious goal of redefining the state-of-the-art on CTC detection. The progress during the project brought the proposed technology idea to form as a competent device. However, more efforts and time are required to have the technology to be implemented at its full potential and to define the state-of-the-art in this rapidly growing and evolving technology field.