Objetivo Cells with stem cell potential, this is with the ability to self-renew and generate specialised progeny, exist in the adult mammalian spinal cord. Previous studies localised this potential within the ependymal cell (EC) population. However, ECs are rather heterogeneous based on their morphological features and the expression of a handful of neural stem cell markers. In this interdisciplinary research proposal, I aim to uncover the cellular and molecular heterogeneity of ECs at the level of individual cells. I propose to take advantage of cutting-edge single-cell RNA-sequencing technology to obtain the transcriptomes of individual ECs from the spinal cord of adult mice. Using advanced computational methods, I will establish EC types and states and use pseudotemporal ordering to elucidate potential lineage relationships among ECs. I will then validate these findings in the tissue context, using high-resolution confocal microscopy. This first comprehensive characterisation of spinal cord ECs will provide novel and fundamental insights into how ECs possess and maintain their unique self-renewing properties. In the future, this will facilitate realisation of the potential of spinal cord stem cells for therapeutic purposes. Ámbito científico medical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencescomputer and information sciencescomputational sciencenatural sciencesphysical sciencesopticsmicroscopyconfocal microscopy Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2016 - Individual Fellowships Convocatoria de propuestas H2020-MSCA-IF-2016 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-ST - Standard EF Coordinador UNIVERSITY OF DUNDEE Aportación neta de la UEn € 183 454,80 Dirección Nethergate DD1 4HN Dundee Reino Unido Ver en el mapa Región Scotland Eastern Scotland Angus and Dundee City Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 183 454,80
