Objective
Temperature is crucial to plants’ growth and development. The cold and heat stresses may drastically inhibit plant growth and cause yield losses in crops. Understanding the temperature responding mechanism is crucial to adapt the crops to withstand extreme temperature changes caused by global warming. Among other temperature regulation mechanism, RNA thermometers (RNATs) can instantaneously respond to temperature shift and directly control translational efficiency therefore protein abundance. To date, no RNAT has been identified in plant mainly due to the fact that instead of sequence level conservation, RNATs only have structure level conservation; also because in-vivo RNA structure probing used to be technically challenging. The host lab has developed a powerful platform for in vivo RNA secondary structure probing at both targeted individual RNA and genome-wide scale. This proposed study aims to globally identify RNA thermometers in plants for the first time, and to quantitatively elucidate the role of RNA structure in the post-transcriptional regulation of gene expression in response to temperature. I will measure the alteration of in vivo RNA structural features in Arabidopsis thaliana under different temperature regime. Additionally, Ribosome profiling data and RNA-Seq data will be combined to study the translation efficiency under the corresponding temperature. Together with the identified significantly changed RNA structure elements, we will be able to determine the putative RNAT. Finally, I will validate the predicted RNATs through studying nature variations. I will perform in-vivo RNA structure probing and determine whether the identified single nucleotide variations (SNV) will have significant effects on temperature-regulatory elements. Besides the potential for crop improvement, our approach to globally measure RNA secondary structure and the corresponding translation efficiency will be easily applied to other organisms and various environmental stimuli.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences computer and information sciences data science data mining
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
NR4 7UH NORWICH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.