Periodic Reporting for period 2 - MicroMOUPE (Microscopy - Making optimal use of photons and electrons)
Reporting period: 2019-09-01 to 2021-02-28
Wave-front shaping: On the one hand, optical wave-front shaping techniques will be used to adapt a microscope to a specific sample, optimizing sensitivity throughout the field of view. On the other hand a wave-front shaping tool based on electron-light interaction will be developed for electron beams, opening up new possibilities for electron optics.
Cavity enhancement: Letting a probe particle interact with a sample multiple times, multiplies the signal, contrast, and signal/noise that is to be expected from such a measurement. This has obvious applications when it comes to the detection of faint signals. In addition to that, multi-passing also increases the signal/noise per interaction of a probe-particle with the sample, which is beneficial for fragile biological samples, where dose has to be minimized. Within the MICROMOUPE project full field of view cavity enhanced microscopes are being developed for optical protein detection, as well as for electron microscopy.
Within these first 18 months, two optical microscopes were designed and set-up, one of them for pushing sensitivity limits of multi-pass microscopy, the other one for sensitivity enhanced measurements using wave-front shaping. The latter one is sketched in Figure 1: In a simple add-on to a commercial microscope, a spatial light modulator allows optimizing the microscope for a specific sample. Both microscopes are currently under test and are optimized with respect to their performance. In addition to that, different cavity enhanced microscopy schemes were analyzed theoretically.
When it comes to electron microscopy, a setup was installed that should allow us to test wave-front shaping based on ponderomotive potentials. Currently all necessary components of the setup are being tested individually, their full integration is one of the main goals for the next period. Additionally, an electron optical design for a first multi-pass TEM was finalized in co-operation with Stanford University and the QEM collaboration. This first prototype is now being built.
- Demonstration of cavity enhanced optical detection of single proteins beyond the single-pass shot-noise limit.
- Realization of a wave-front shaping device for electrons.
- Demonstration of a first prototype of a multi-pass transmission electron microscope.