Micro-nano fibres assembled in an aligned, single-layered array can exhibit excellent transparency due to the low level of light absorption associated with individual fibre thickness. Building two-dimensional (2D) and three-dimensional (3D) architects using these fibre array could offer exciting prospects for applications spanning from sensors, to tissue engineering scaffolds. Existing fibre spinning techniques have been tailored for producing micro-to-nano scale fibers, but can be restrictive in the design of macroscopic fiber architectures in the 3D space, and the choice of fibre materials. To address these technological gaps, we report two new fibre spinning techniques: first, 3D-LEP which combines low voltage electrospinning (LEP) and additive manufacturing to pattern suspended fiber layers in multiple tiers and designable orientations; and secondly, an efficient inflight fluidic fibre deposition process to produce conducting fibres. Using these techniques, we demonstrate biological applications utilising fiber topography to guide the assembly of cellular aggregates in a 3D culture context; optoelectronic applications showing unconventional transparent, suspended fibre-array electronics; and a high performance, self-powered acoustic sensor based on a piezoelectric polymer.