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Synthetic Circuits for Robust Orthogonal Production

Deliverables

Theory for robust circuit design with optimal specific fluxes

Theory for robust circuit design with optimal specific fluxes

Chromosomally located fluorescently-tagged expression strains

Chromosomally located fluorescently-tagged expression strains

Map of the position of translating ribosomes in S. cerevisiae and in E. coli when expressing different production circuits

Map of the position of translating ribosomes in S. cerevisiae and in E. coli when expressing different production circuits

Data set of single-cell of stress-responsive transcription factors

Data set of single-cell of stress-responsive transcription factors

Project website

Project website

Map of charged tRNAome in S. cerevisiae and in E. coli when expressing different production circuits

Map of charged tRNAome in S. cerevisiae and in E. coli when expressing different production circuits

Parameterised kinetic model of E. coli glycolysis/gluconeogenesis and pentose phosphate pathway

Parameterised kinetic model of E. coli glycolysis/gluconeogenesis and pentose phosphate pathway

Data set of single-cell measurements of global physiological variables

Data set of single-cell measurements of global physiological variables

Panel of biosensors directed to specific locations in the cell (organelles in S. cerevisiae; cytosol or membrane of E. coli)

Panel of biosensors directed to specific locations in the cell (organelles in S. cerevisiae; cytosol or membrane of E. coli)

Mid-term special session at an international conference CF1

Mid-term special session at an international conference CF1 Co-responsible partner BSY

Overall Recruitment

Overall Recruitment of ESR All partners reponsible

Researcher Declarations on Conformity

Researcher Declarations on Conformity All partners co-responsible

Transferable skills courses

Transferable skills courses months: 7, 12, 18, 21

Scientific courses

Scientific courses Months: 7, 10, 12, 21, 24

Supervisory Board constitution

Constitution of the supervisory board

Systems for monitoring and quantifying crowding stress

Systems for monitoring and quantifying crowding stress

Searching for OpenAIRE data...

Publications

CRISPR/Cas12a Multiplex Genome Editing of <em>Saccharomyces cerevisiae</em> and the Creation of Yeast Pixel Art

Author(s): Klaudia Ciurkot, Brenda Vonk, Thomas E. Gorochowski, Johannes A. Roubos, René Verwaal
Published in: Journal of Visualized Experiments, Issue 147, 2019, ISSN 1940-087X
DOI: 10.3791/59350

Absolute quantification of translational regulation and burden using combined sequencing approaches

Author(s): Thomas E Gorochowski, Irina Chelysheva, Mette Eriksen, Priyanka Nair, Steen Pedersen, Zoya Ignatova
Published in: Molecular Systems Biology, Issue 15/5, 2019, ISSN 1744-4292
DOI: 10.15252/msb.20188719

Harnessing the metabolic potential of Streptococcus thermophilus for new biotechnological applications

Author(s): Sofia Markakiou, Paula Gaspar, Eric Johansen, Ahmad A Zeidan, Ana Rute Neves
Published in: Current Opinion in Biotechnology, Issue 61, 2020, Page(s) 142-152, ISSN 0958-1669
DOI: 10.1016/j.copbio.2019.12.019

Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells

Author(s): Boqun Liu, Sara N. Mavrova, Jonas van den Berg, Sebastian K. Kristensen, Luca Mantovanelli, Liesbeth M. Veenhoff, Bert Poolman, Arnold J. Boersma
Published in: ACS Sensors, Issue 3/9, 2018, Page(s) 1735-1742, ISSN 2379-3694
DOI: 10.1021/acssensors.8b00473

Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression

Author(s): Amedea Perfumo, Georg Johannes Freiherr von Sass, Eva-Lena Nordmann, Nediljko Budisa, Dirk Wagner
Published in: Frontiers in Microbiology, Issue 11, 2020, ISSN 1664-302X
DOI: 10.3389/fmicb.2020.00881

Synthesis of New Aza‐ and Thia‐Crown Ether Based Amino Acids

Author(s): Tobias Schneider, Nico Brüssow, Alev Yuvanc, Nediljko Budisa
Published in: ChemistrySelect, Issue 5/9, 2020, Page(s) 2854-2857, ISSN 2365-6549
DOI: 10.1002/slct.202000122