Periodic Reporting for period 1 - PhotoBioCat (Light-driven sustainable biocatalysis training network)
Reporting period: 2018-01-01 to 2019-12-31
Work on cyanobacterial whole-cell biocatalysts allowed to expand the scope of the method towards novel enzyme classes, including light-driven imine-reduction for the synthesis of chiral amines. This part of the work generated model systems to compare different enzyme classes and the utilization of different redox cofactors. The first phase of the project was also used to investigate optimal reaction conditions for cyanobacterial biotransformations and to generate mutants that are expected to be improved chassis for whole-cell biocatalysis. The second phase will focus on the integration of the different enzymes to the chassis and to aim for a substantial improvement of productivity and space-time-yield of the reactions of interest.
Work on photobiocatalysis in cell-free systems compared light-driven enzymes with indirect photobiocatalysis using organic and inorganic photocatalysts. The ESRs explored new reaction concepts for the coupling of highly selective enzymes to light-driven supply of redox cofactors. Work spans from the coupling of bacterial whole-cell biocatalysts to photocatalysts in the cell-free space to the investigation of photosynthesis in cell-free systems. After the establishment of the reaction systems and the proof-of-concept, research in the second phase will be dedicated to a detailed investigation of the electron transfer and the development of strategies for an optimization of the reactions at different scales.
The work on the scale-up and intensification focused on different photobiocatalytic strategies for the synthesis of a platform chemical, including light-driven biotransformation in vivo and two different routes using in vitro photobiocatalysis. The results so far highlighted key parameters of the reaction conditions. In the scale-up, the investigation of different reactor concepts is considered crucial for an optimal supply of light for the light-harvesting systems.