Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells

Livrables

Report on action of SARS-CoV-2 infection on liver ultrastructure

Report on action of SARSCoV2 infection on liver ultrastructure delivered to the European Commission

Report and analysis summarizing comb. SOFI/TP-SIM for tissue imaging

Report summarizing comb SOFITPSIM for tissue imaging delivered to European Commission

3D extension of open source fairSIM image reconstruction software

"Public release of open access image reconstruction software ""fairSIM"" with extension for the reconstruction of 3D image data"

Training agenda

Training agenda written, distributed to partners, and published on DeLIVER website

Report summarizing imaging results of rodent and human liver tissue
Report on the effects of mechanical stress on LSEC morphology
Report detailing differences between normal liver tissue and diseased tissue
Report/dataset of highest res. imaging of intact liver sinusoids

Reportdataset of highest res imaging of intact liver sinusoids delivered to European Commission

Report on the action of small molecules on LSEC morphology
Nonlinear image reconstruction implemented in fairSIM

"Nonlinear image reconstruction implemented in public release of open access software ""fairSIM"""

Report on dynamics rates of fenestrations in isolated LSEC

Report on fenestration dynamics in isolated liver endothelial cells as measured by optical nanoscopy

DeLIVER website

Website ready and launched

Datasets of rodent and human liver tissue nanoscopy

Data Management Plan

Data management plan for the Open Data Research Pilot submitted

Publications

Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

Auteurs: Christian Pilger, Jakub Pospíšil, Marcel Müller, Martin Ruoff, Martin Schütte, Heinrich Spiecker, Thomas Huser
Publié dans: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Numéro 379/2199, 2021, Page(s) 20200300, ISSN 1364-503X
Éditeur: Royal Society of London
DOI: 10.1098/rsta.2020.0300

Multi-moded high-index contrast optical waveguide for super-contrast high-resolution label-free microscopy

Auteurs: Nikhil Jayakumar, Firehun T. Dullo, Vishesh Dubey, Azeem Ahmad, Florian Ströhl, Jennifer Cauzzo, Eduarda Mazagao Guerreiro, Omri Snir, Natasa Skalko-Basnet, Krishna Agarwal and Balpreet Singh Ahluwalia
Publié dans: Nanophotonics, Numéro 21928614, 2022, Page(s) 3421–3436, ISSN 2192-8614
Éditeur: De Gruyter
DOI: 10.1515/nanoph-2022-0100

Editorial: Roles of Liver Sinusoidal Endothelial Cells in Liver Homeostasis and Disease

Auteurs: Patricia F. Lalor, Thomas Huser, and Leo A. van Grunsven
Publié dans: Frontiers in Physiology, Numéro 16624548, 2021, Page(s) 869473-1 - 869473-3, ISSN 1662-4548
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fphys.2022.869473

Hepatic Vasculopathy and Regenerative Responses of the Liver in Fatal Cases of COVID-19

Auteurs: Barbara Kaltschmidt, Antonia D.E. Fitzek, Julia Schaedler, Christine Förster, Christian Kaltschmidt, Torsten Hansen, Fabian Steinfurth, Beatrice A. Windmöller, Christian Pilger, Cihang Kong, Kashika Singh, Axel Nierhaus, Dominic Wichmann, Jan Sperhake, Klaus Püschel, Thomas Huser, Martin Krüger, Simon C. Robson, Ludwig Wilkens, Jan Schulte am Esch
Publié dans: Clinical Gastroenterology and Hepatology, Numéro 19/8, 2021, Page(s) 1726-1729.e3, ISSN 1542-3565
Éditeur: W. B. Saunders Co., Ltd.
DOI: 10.1016/j.cgh.2021.01.044

Gene Signatures Detect Damaged Liver Sinusoidal Endothelial Cells in Chronic Liver Diseases.

Auteurs: Stefaan Verhulst; Elise Anne van Os; Vincent De Smet; Nathalie Eysackers; Inge Mannaerts; Leo A. van Grunsven
Publié dans: Frontiers in Medicine, Numéro 16624548, 2021, Page(s) 750044-1 - 750044-14, ISSN 1662-4548
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fmed.2021.750044

Fluorescence fluctuations-based super-resolution microscopy techniques: an experimental comparative study

Auteurs: Opstad, Ida S.; Acuña, Sebastian; Hernandez, Luís Enrique Villegas; Cauzzo, Jennifer; Škalko-Basnet, Nataša; Ahluwalia, Balpreet S.; Agarwal, Krishna
Publié dans: Optics Express, Numéro 10944087, 2021, Page(s) 23368-23380, ISSN 1094-4087
Éditeur: Optical Society of America
DOI: 10.1364/oe.423809

Characterization of Liposomes Using Quantitative Phase Microscopy (QPM)

Auteurs: Jennifer Cauzzo, Nikhil Jayakumar, Balpreet Singh Ahluwalia, Azeem Ahmad, Nataša Škalko-Basnet
Publié dans: Pharmaceutics, Numéro 13/5, 2021, Page(s) 590, ISSN 1999-4923
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13050590

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Auteurs: Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Publié dans: Advanced Science, Numéro Volume 8, Numéro 19, 2021, ISSN 2198-3844
Éditeur: Weinheim : WILEY-VCH, [2014]-
DOI: 10.1002/advs.202100798

Following the Fate of Dye-Containing Liposomes In Vitro

Auteurs: Jennifer Cauzzo, Mona Nystad, Ann Mari Holsæter, Purusotam Basnet, Nataša Škalko-Basnet
Publié dans: International Journal of Molecular Sciences, Numéro 21/14, 2020, Page(s) 4847, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21144847

Primary rat LSECs preserve their characteristic phenotype after cryopreservation

Auteurs: Viola Mönkemöller, Hong Mao, Wolfgang Hübner, Gianina Dumitriu, Peter Heimann, Gahl Levy, Thomas Huser, Barbara Kaltschmidt, Christian Kaltschmidt, Cristina I. Øie
Publié dans: Scientific Reports, Numéro 8/1, 2018, Page(s) 10, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-018-32103-z

Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

Auteurs: Andreas Markwirth, Mario Lachetta, Viola Mönkemöller, Rainer Heintzmann, Wolfgang Hübner, Thomas Huser, Marcel Müller
Publié dans: Nature Communications, Numéro 10/1, 2019, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-019-12165-x

Sampling moiré method: a tool for sensing quadratic phase distortion and its correction for accurate quantitative phase microscopy

Auteurs: NIKHIL JAYAKUMAR, Azeem Ahmad, Dalip Mehta, Balpreet Ahluwalia
Publié dans: Optics Express, 2020, ISSN 1094-4087
Éditeur: Optical Society of America
DOI: 10.1364/oe.383461

High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Auteurs: Adrien Descloux, Marcel Müller, Vytautas Navikas, Andreas Markwirth, Robin van den Eynde, Tomas Lukes, Wolfgang Hübner, Theo Lasser, Aleksandra Radenovic, Peter Dedecker, Thomas Huser
Publié dans: Nanophotonics, Numéro 9/1, 2019, Page(s) 143-148, ISSN 2192-8614
Éditeur: De Gruyter
DOI: 10.1515/nanoph-2019-0346

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

Auteurs: Cihang Kong, Christian Pilger, Henning Hachmeister, Xiaoming Wei, Tom H. Cheung, Cora S. W. Lai, Nikki P. Lee, Kevin. K. Tsia, Kenneth K. Y. Wong, Thomas Huser
Publié dans: Light: Science & Applications, Numéro 9/1, 2020, ISSN 2047-7538
Éditeur: Nature Publishing Group
DOI: 10.1038/s41377-020-0259-2

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Auteurs: Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Publié dans: Journal of Physics: Photonics, Numéro 1/4, 2019, Page(s) 044001, ISSN 2515-7647
Éditeur: IOP Science
DOI: 10.1088/2515-7647/ab36ae

Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices

Auteurs: Alexander H. McMillan, Emma K. Thomée, Alessandra Dellaquila, Hussam Nassman, Tatiana Segura, Sasha Cai Lesher-Pérez
Publié dans: Micromachines, Numéro 11/8, 2020, Page(s) 731, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi11080731

Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study.

Auteurs: K. Szafranska; Christopher Florian Holte; L.D. Kruse; Hong Mao; Cristina Ionica Øie; Marek Szymonski; Bartlomiej Zapotoczny; Peter McCourt
Publié dans: Micron, Numéro 0361526X, 2021, Page(s) 103121, ISSN 0361-526X
Éditeur: Haworth Press Inc.
DOI: 10.1016/j.micron.2021.103121

On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination

Auteurs: Nikhil Jayakumar, Øystein I. Helle, Krishna Agarwal, Balpreet Singh Ahluwalia
Publié dans: Optics Express, Numéro 28/24, 2020, Page(s) 35454, ISSN 1094-4087
Éditeur: Optical Society of America
DOI: 10.1364/oe.403804

The wHole Story About Fenestrations in LSEC

Auteurs: Karolina Szafranska, Larissa D. Kruse, Christopher Florian Holte, Peter McCourt, and Bartlomiej Zapotoczny
Publié dans: Frontiers in Physiology, Numéro 16624548, 2021, Page(s) 735573-1 - 735573-25, ISSN 1662-4548
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fphys.2021.735573

Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies.

Auteurs: Cihang Kong; Stefanie Bobe; Christian Pilger; Mario Lachetta; Cristina Ionica Øie; Nils Kirschnick; Viola Mönkemöller; Wolfgang Hübner; Wolfgang Hübner; Christine Förster; Mark Schüttpelz; Friedemann Kiefer; Thomas Huser; Thomas Huser; Jan Schulte am Esch; Jan Schulte am Esch
Publié dans: Frontiers in Physiology, Numéro 16624548, 2021, Page(s) 637136-1 - 637136-15, ISSN 1662-4548
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fphys.2021.637136

Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells.

Auteurs: Ankit Butola; David A. Coucheron; Karolina Szafranska; Azeem Ahmad; Hong Mao; Jean-Claude Tinguely; Peter McCourt; Paramasivam Senthilkumaran; Dalip Singh Mehta; Krishna Agarwal; Balpreet Singh Ahluwalia
Publié dans: Proceedings of the National Academy of Science of the U.S.A., Numéro 00278424, 2021, Page(s) e2115323118, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.2115323118

Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin

Auteurs: Ezequiel Miron, Roel Oldenkamp, Jill M. Brown, David M. S. Pinto, C. Shan Xu, Ana R. Faria, Haitham A. Shaban, James D. P. Rhodes, Cassandravictoria Innocent, Sara de Ornellas, Harald F. Hess, Veronica Buckle, Lothar Schermelleh
Publié dans: Science Advances, Numéro 6/39, 2020, Page(s) eaba8811, ISSN 2375-2548
Éditeur: American Association for the Advancement of Science
DOI: 10.1126/sciadv.aba8811

From fixed-dried to wet-fixed to live – comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations

Auteurs: Karolina Szafranska, Tanja Neuman, Zbigniew Baster, Zenon Rajfur, Oskar Szelest, Christopher Holte, Agata Kubisiak, Edyta Kuś, Deanna L. Wolfson, Stefan Chlopicki, Balpreet S. Ahluwalia, Malgorzata Lekka, Marek Szymonski, Peter McCourt and Bartlomiej Zapotoczny
Publié dans: Nanophotonics, Numéro 21928614, 2022, Page(s) 2253-2270, ISSN 2192-8614
Éditeur: De Gruyter
DOI: 10.1515/nanoph-2021-0818

The Contribution of Liver Sinusoidal Endothelial Cells to Clearance of Therapeutic Antibody

Auteurs: Bethany H. James, Pantelitsa Papakyriacou, Matthew J. Gardener, Louise Gliddon, Christopher J. Weston, and Patricia F. Lalor
Publié dans: Frontiers in Physiology, Numéro 16624548, 2021, Page(s) 753833-1 - 753833-14, ISSN 1662-4548
Éditeur: Frontiers Research Foundation
DOI: 10.3389/fphys.2021.753833

Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components

Auteurs: Jakub Pospíšil, Gerd Wiebusch, Karel Fliegel, Miloš Klíma, Thomas Huser
Publié dans: Optics Express, Numéro 29/8, 2021, Page(s) 11833, ISSN 1094-4087
Éditeur: Optical Society of America
DOI: 10.1364/oe.420592

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