Shaping the bacterial cell wall: the actin-like cytoskeleton, from single molecules to morphogenesis and antimicrobials

Project Information

BACTIN

Grant agreement ID: 772178

DOI

10.3030/772178

EC signature date 28 March 2018

Start date 1 February 2019

End date 31 January 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 902 195,00

EU contribution

€ 1 902 195,00

Coordinated by

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
France

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (12)

Magnesium rescues the morphology of Bacillus subtilis mreB mutants through its inhibitory effect on peptidoglycan hydrolases

Author(s): Benoit Tesson, Alex Dajkovic, Ruth Keary, Christian Marlière, Christine C Dupont-Gillain, Rut Carballido-López
Published in: Scientific Reports, 2022, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-04294-5

Quantification of membrane fluidity in bacteria using TIR-FCS

Author(s): Aurélien Barbotin, Cyrille Billaudeau, Erdinc Sezgin, Rut Carballido-López
Published in: Biophysical Journal, Issue 123, 2024, Page(s) 2484-2495, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2024.06.012

Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria

Author(s): Filipe Carvalho, Alexis Carreaux, Anna Sartori-Rupp, Stéphane Tachon, Anastasia D. Gazi, Pascal Courtin, Pierre Nicolas, Florence Dubois-Brissonnet, Aurélien Barbotin, Emma Desgranges, Matthieu Bertrand, Karine Gloux, Catherine Schouler, Rut Carballido-López, Marie-Pierre Chapot-Chartier, Eliane Milohanic, Hélène Bierne, Alessandro Pagliuso
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-52633-7

Real-time nanoscale investigation of spore coat assembly in Bacillus subtilis

Author(s): Armand Lablaine, Dimitri Juillot, Ciarán Condon, Rut Carballido-López
Published in: Communications Biology, Issue 8, 2025, ISSN 2399-3642
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s42003-025-08522-w

Embedding a ribonuclease in the spore crust couples gene expression to spore development in <i>Bacillus subtilis</i>

Author(s): Alexandre D’Halluin, Laetitia Gilet, Armand Lablaine, Olivier Pellegrini, Mónica Serrano, Anastasia Tolcan, Magali Ventroux, Sylvain Durand, Marion Hamon, Adriano O Henriques, Rut Carballido-López, Ciarán Condon
Published in: Nucleic Acids Research, Issue 53, 2025, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae1301

Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex

Author(s): Dimitri Juillot, Cyrille Billaudeau, Isabelle Mortier-Barrière, Aurélien Barbotin, Armand Lablaine, Patrice Polard, Nathalie Campo, Rut Carballido-López
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-60600-z

New PALM-compatible integration vectors for use in the Gram-positive model bacterium <i>Bacillus subtilis</i>

Author(s): Ipek Altinoglu, Rut Carballido-Lopez
Published in: Microbiology Spectrum, Issue 12, 2024, ISSN 2165-0497
Publisher: American Society for Microbiology
DOI: 10.1128/spectrum.01619-24

A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus

Author(s): Armand Lablaine, Stéphanie Chamot, Mónica Serrano, Cyrille Billaudeau, Isabelle Bornard, Rut Carballido-López, Frédéric Carlin, Adriano O Henriques, Véronique Broussolle
Published in: Scientific Reports, Issue 13, 2023, Page(s) 15136, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-42143-9

Rod width under control

Author(s): Rut Carballido-López
Published in: Nature Microbiology, Issue 4/8, 2019, Page(s) 1246-1248, ISSN 2058-5276
Publisher: Nature Publishing Group
DOI: 10.1038/s41564-019-0528-0

DivIVA controls the dynamics of septum splitting and cell elongation in<i>Streptococcus pneumoniae</i>

Author(s): Jennyfer Trouve, André Zapun, Laure Bellard, Dimitri Juillot, Anais Pelletier, Celine Freton, Morgane Baudoin, Rut Carballido-Lopez, Nathalie Campo, Yung-Sing Wong, Christophe Grangeasse, Cecile Morlot
Published in: mBio, 2024, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1101/2024.05.09.593393

On the role of nucleotides and lipids in the polymerization of the actin homolog MreB from a Gram-positive bacterium

Author(s): Wei Mao, Lars D. Renner, Charlène Cornilleau, Ines Li de la Sierra-Gallay, Sana Afensiss, Sarah Benlamara, Yoan Ah-Seng, Herman Van Tilbeurgh, Sylvie Nessler, Aurélie Bertin, Arnaud Chastanet, Rut Carballido-López
Published in: eLife, Issue 12, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.84505

A High-Content Microscopy Screening Identifies New Genes Involved in Cell Width Control in Bacillus subtilis

Author(s): Dimitri Juillot, Charlène Cornilleau, Nathalie Deboosere, Cyrille Billaudeau, Parfait Evouna-Mengue, Véronique Lejard, Priscille Brodin, Rut Carballido-López* and Arnaud Chastanet*
Published in: mSystems, Issue Vol. 6, No. 6 / bimothly, 2021, Page(s) 6(6):e0101721, ISSN 2379-5077
Publisher: American Society for Microbiology
DOI: 10.1128/msystems.01017-21

Other (6)

Quantification of membrane fluidity in bacteria using TIR-FCS

Author(s): Aurélien Barbotin, Cyrille Billaudeau, Erdinc Sezgin, RUT CARBALLIDO LOPEZ
Published in: BioRxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.10.13.562271

Comprehensive and Comparative Transcriptional Profiling of the Cell Wall Stress Response in Bacillus subtilis

Author(s): Qian Zhang, Charlene Cornilleau, Raphael R. Müller, Doreen Meier, Pierre Flores, Cyprien Guérin, Diana Wolf, Vincent Fromion, Rut Carballido-Lopez, Thorsten Mascher
Published in: BioRxiv, 2023
Publisher: Cold Spring Harbor
DOI: 10.1101/2023.02.03.526509

Polymerization cycle of actin homolog MreB from a Gram-positive bacterium

Author(s): Wei Mao, Lars D. Renner, Charlène Cornilleau, Ines Li de la Sierra-Gallay, Sarah Benlamara, Yoan Ah-Seng, Herman Van Tilbeurgh, Sylvie Nessler, Aurélie Bertin, Arnaud Chastanet, Rut Carballido-López
Published in: BioRxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.10.19.512861

Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex

Author(s): Dimitri Juillot, Cyrille Billaudeau, Aurélien Barbotin, Armand Lablaine, Isabelle Mortier-Barrière, Patrice Polard, Nathalie Campo and Rut Carballido-Lopez
Published in: BioRxiv, 2024
Publisher: BioRxiv
DOI: 10.1101/2024.02.28.582556

Peptidoglycan remodeling in response to cell wall acting antibiotics in Bacillus subtilis

Author(s): Charlène Cornilleau, Laura Alvarez, Christine Wegler, Cyrille Billaudeau,Felipe Cava, Rut Carballido-López
Published in: BioRxiv, 2023
Publisher: Cold Spring Harvor Laboratory
DOI: 10.1101/2023.01.23.525174

Real-Time Nanoscale Investigation of Spore Coat Assembly in<i>Bacillus subtilis</i>

Author(s): Armand Lablaine, Dimitri Juillot, Ciarán Condon, Rut Carballido-López
Published in: BioRxiv, 2024
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.12.19.629407

Non-peer reviewed articles (2)

Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy

Author(s): Charlène Cornilleau, Arnaud Chastanet, Cyrille Billaudeau, Rut Carballido-López
Published in: Cytoskeleton Dynamics - Methods and Protocols, Issue 2101, 2020, Page(s) 123-133, ISSN 1940-6029, ISBN 978-1-0716-0218-8
Publisher: Clifton, N.J. : Humana Press
DOI: 10.1007/978-1-0716-0219-5_8

Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-Like Assemblies

Author(s): Cyrille Billaudeau, Arnaud Chastanet, Rut Carballido-López
Published in: Cytoskeleton Dynamics - Methods and Protocols, Issue 2101, 2020, Page(s) 135-145, ISSN 1940-6029, ISBN 978-1-0716-0218-8
Publisher: Clifton, N.J. : Humana Press
DOI: 10.1007/978-1-0716-0219-5_9