Engineered Protein Nanosheets at Liquid-Liquid Interfaces for Stem Cell Expansion, Sorting and Tissue Engineering

Project Information

ProLiCell

Grant agreement ID: 772462

DOI

10.3030/772462

Project closed

EC signature date 27 April 2018

Start date 1 September 2018

End date 31 August 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 999 389,00

EU contribution

€ 1 999 389,00

Coordinated by

QUEEN MARY UNIVERSITY OF LONDON
United Kingdom

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (21)

Supercharged Protein Nanosheets for Cell Expansion on Bioemulsions

Author(s): Alexandra Chrysanthou; Hassan Kanso; Wencheng Zhong; Li Shang; Julien E. Gautrot
Published in: ACS Applied Materials and Inverfaces, 2023, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.2c20188

Responsive Polymer Brush Design and Emerging Applications for Nanotheranostics

Author(s): Danyang Li, Lizhou Xu, Jing Wang, Julien E. Gautrot
Published in: Advanced Healthcare Materials, Issue 10/5, 2021, Page(s) 2000953, ISSN 2192-2640
Publisher: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202000953

Co-Surfactant-Free Bioactive Protein Nanosheets for the Stabilization of Bioemulsions Enabling Adherent Cell Expansion

Author(s): Alexandra Chrysanthou; Minerva Bosch-Fortea; Julien E. Gautrot
Published in: Biomacromolecules, 2023, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.2c01289

Photo-Configurable, Cell-Remodelable Disulfide Crosslinked Hyaluronic Acid Hydrogels

Author(s): Wu, L.; Di Cio, S.; Azevedo, H. and Gautrot, J.E.
Published in: Biomacromolecules, 2020, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.0c00603

Interfacial mechanics of β-casein and albumin mixed protein assemblies at liquid-liquid interfaces

Author(s): Alexandra Chrysanthou, Minerva Bosch-Fortea, Clemence Nadal, Ali Zarbakhsh, Julien E. Gautrot
Published in: Journal of Colloid and Interface Science, Issue 674, 2024, Page(s) 379-391, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2024.06.111

The Architecture of Oligonucleotide‐Polycationic Brush Complexes – A Neutron Reflectometry Study

Author(s): Julien E. Gautrot, Lan Chang, Cardee Alexis, Philipp Gutfreund, Ali Zarbakhsh
Published in: Advanced Materials Interfaces, Issue 9, 2023, Page(s) 2201344, ISSN 2196-7350
Publisher: Wiley
DOI: 10.1002/admi.202201344

Competitive binding and molecular crowding regulate the cytoplasmic interactome of non-viral polymeric gene delivery vectors

Author(s): Aji Alex M. Raynold, Danyang Li, Lan Chang, Julien E. Gautrot
Published in: Nature Communications, Issue 12, 2022, Page(s) 6445, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-26695-w

Vascularised cardiac spheroids-on-a-chip for testing the toxicity of therapeutics

Author(s): Stefania Di Cio, Emilie Marhuenda, Malcolm Haddrick, Julien E. Gautrot
Published in: Scientific Reports, Issue 14, 2024, Page(s) 3370, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-024-53678-w

The impact of pericytes on the stability of microvascular networks in response to nanoparticles

Author(s): Matthew Dibble, Stefania Di Cio’, Piaopiao Luo, Frances Balkwill, Julien E. Gautrot
Published in: Scientific Reports, Issue 13, 2023, Page(s) 5729, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-31352-x

Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model

Author(s): Christian F. E. Jones, Stefania Di Cio, John T. Connelly, Julien E. Gautrot
Published in: Frontiers in Bioengineering and Biotechnology, Issue 10, 2022, Page(s) 915702, ISSN 2296-4185
Publisher: Frontiersin
DOI: 10.3389/fbioe.2022.915702

Modulation of Thiol–Ene Coupling by the Molecular Environment of Polymer Backbones for Hydrogel Formation and Cell Encapsulation

Author(s): Burcu Colak, Linke Wu, Edward J. Cozens, Julien E. Gautrot
Published in: ACS Applied Bio Materials, Issue 3/9, 2020, Page(s) 6497-6509, ISSN 2576-6422
Publisher: ACS
DOI: 10.1021/acsabm.0c00908

Contractile myosin rings and cofilin-mediated actin disassembly orchestrate ECM nanotopography sensing

Author(s): Stefania Di Cio, Thomas Iskratsch, John T. Connelly, Julien E. Gautrot
Published in: Biomaterials, Issue 232, 2020, Page(s) 119683, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2019.119683

Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers

Author(s): Lihui Peng, Julien E. Gautrot
Published in: Materials Today Bio, Issue 12, 2021, Page(s) 100159, ISSN 2590-0064
Publisher: Wiley
DOI: 10.1016/j.mtbio.2021.100159

Extreme reversal in mechanical anisotropy in liquid-liquid interfaces reinforced with self-assembled protein nanosheet

Author(s): Megone, W.; Kong, D.; Peng, L.; Gautrot, J.E.
Published in: J Colloid Interface Science, 2021, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2021.03.055

Impact of the multiscale viscoelasticity of quasi-2D self-assembled protein networks on stem cell expansion at liquid interfaces

Author(s): Dexu Kong, Lihui Peng, Minerva Bosch-Fortea, Alexandra Chrysanthou, Cardee V.J-M. Alexis, Carlos Matellan, Ali Zarbakhsh, Giulia Mastroianni, Armando del Rio Hernandez, Julien E. Gautrot
Published in: Biomaterials, Issue 284, 2022, Page(s) 121494, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2022.121494

Strong Elastic Protein Nanosheets Enable the Culture and Differentiation of Induced Pluripotent Stem Cells on Microdroplets

Author(s): Elijah Mojares, Clemence Nadal, Daniel Hayler, Hassan Kanso, Alexandra Chrysanthou, Carlos E. Neri Cruz, Julien E. Gautrot
Published in: Advanced Materials, Issue 36, 2024, Page(s) 2406333, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202406333

Mesenchymal Stem Cells Sense the Toughness of Nanomaterials and Interfaces

Author(s): Lihui Peng; Carlos Matellan; Minerva Bosch‐Fortea; Jordi Gonzalez‐Molina; Matteo Frigerio; Stefan Salentinig; Armando del Rio Hernandez; Julien E. Gautrot
Published in: Advanced Healthcare Materials, 2023, ISSN 2192-2659
Publisher: World Scientific
DOI: 10.1002/adhm.202203297

Growth of mesenchymal stem cells at the surface of silicone, mineral and plant-based oils

Author(s): Lihui Peng, Clémence Nadal, Julien E Gautrot
Published in: Biomedical Materials, Issue 18, 2023, Page(s) 035005, ISSN 1748-6041
Publisher: Institute of Physics Publishing
DOI: 10.1088/1748-605x/acbdda

Programmed Self-Assembly of DNA Nanosheets with Discrete Single-Molecule Thickness and Interfacial Mechanics: Design, Simulation, and Characterization

Author(s): Keitel Cervantes-Salguero, Yair Augusto Gutiérrez Fosado, William Megone, Julien E. Gautrot, Matteo Palma
Published in: Molecules, Issue 28, 2023, Page(s) 3686, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules28093686

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling

Author(s): Metzli Hernandez Marchena, Elisa Lambert, Bojana Bogdanović, Fauzia Quadir, Carlos E. Neri-Cruz, Jiajun Luo, Clemence Nadal, Elisa Migliorini, Julien E. Gautrot
Published in: ACS Applied Materials & Interfaces, Issue 16, 2024, Page(s) 40455-40468, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.4c05139

A guide to functionalisation and bioconjugation strategies to surface-initiated polymer brushes

Author(s): Carlos Eduardo Neri-Cruz, Franciane Mouradian Emidio Teixeira, Julien E. Gautrot
Published in: Chemical Communications, Issue 59, 2024, Page(s) 7534-7558, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3cc01082a

Other (4)

Biomimetic Artificial Bone Marrow Niches for the Scale Up of Hematopoietic Stem Cells

Author(s): Minerva Bosch-Fortea, Daniele Marciano, Julien E. Gautrot
Published in: Biorxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.06.23.546233

Engineered Protein Nanosheets for the Scale up of Mesenchymal Stem Cell Culture on Bioemulsions

Author(s): Minerva Bosch-Fortea, Clemence Nadal, Alexandra Chrysanthou, Daniele Marciano, Hassan Kanso, Nam Nguyen, Julien E. Gautrot
Published in: Biorxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.12.18.572135

Matrix Nanoscale Mechanics Regulates Exosome Production by Mesenchymal Stem Cells

Author(s): Alexandra Chrysanthou, Julien E. Gautrot
Published in: Biorxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.09.19.558518

The Formation and Architecture of Surface-Initiated Polymer Brush Gene Delivery Complexes

Author(s): Carlos Neri-Cruz, Lan Chang, Franciane Teixeira, Shoghik Hakobyan, Philipp Gutfreund, Mario Campana, Ali Zarbakhsh, Julien Gautrot
Published in: Chemrxiv, 2024
Publisher: American Chemical Society (ACS)
DOI: 10.26434/chemrxiv-2024-1bpwh

Intellectual Property Rights

Patent (1)

METHODS AND SYSTEMS FOR THE CULTURE OF CELLS AT LIQUID-LIQUID INTERFACES

Application/Publication number: 20 23058935
Date: 2023-04-05
Applicant(s): QUEEN MARY UNIVERSITY OF LONDON