High speed AFM imaging of molecular processes inside living cells

Project Information

InCell

Grant agreement ID: 773091

DOI

10.3030/773091

Project closed

EC signature date 6 March 2018

Start date 1 April 2018

End date 30 September 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 999 925,00

EU contribution

€ 1 999 925,00

Coordinated by

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Switzerland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (21)

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Author(s): Vytautas Navikas; Samuel M. Leitao; Kristin S. Grussmayer; Kristin S. Grussmayer; Adrien Descloux; Barney Drake; Klaus Yserentant; Philipp Werther; Dirk-Peter Herten; Richard Wombacher; Richard Wombacher; Aleksandra Radenovic; Georg E. Fantner
Published in: Nature Communications, Issue 20411723, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-24901-3

Integration of sharp silicon nitride tips into high-speed SU8 cantilevers in a batch fabrication process

Author(s): Nahid Hosseini, Matthias Neuenschwander, Oliver Peric, Santiago H Andany, Jonathan D Adams, Georg E Fantner
Published in: Beilstein Journal of Nanotechnology, Issue 10, 2019, Page(s) 2357-2363, ISSN 2190-4286
Publisher: Beilstein-Institut Zur Forderung der Chemischen Wissenschaften
DOI: 10.3762/bjnano.10.226

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds

Author(s): S. M. Leitao, B. Drake & G. E. Fantner, V. Navikas, H. Miljkovic, S. Marion, S. F. Mayer & A. Radenovic,G. Pistoletti Blanchet & A. Kuhn,K. Chen & U. F. Keyser
Published in: Nature Nanotechnology, Issue 18, 1078–1084, 2023, ISSN 1748-3387
Publisher: Nature Publishing Group
DOI: 10.1038/s41565-023-01412-4

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Author(s): Hatzopoulos, Georgios N. ; Kuekenshoener, Tim ; Banterle, Niccolo ; Favez, Tatiana ; Flueckiger, Isabelle ; Hamel, Virginie ; Andany, Santiago ; Fantner, Georg E. ; Hantschel, Oliver ; Goenczy, Pierre
Published in: Nature Communications, 2021, Page(s) 3805, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-23897-0

Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities

Author(s): Zrinko Baričević, Zahra Ayar, Samuel M. Leitao, Miranda Mladinic, Georg E. Fantner Jelena Ban
Published in: Advanced Optical Sensing Techniques for Applications in Biomedicine, Issue 13/3, 2023, Page(s) 404, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13030404

Scratching the Surface: Bacterial Cell Envelopes at the Nanoscale

Author(s): Albertus Viljoen, Simon J. Foster, Georg E. Fantner, Jamie K. Hobbs, Yves F. Dufrêne
Published in: mBio, Issue 11/1, 2020, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.03020-19

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Author(s): Banterle, Niccolo; Nievergelt, Adrian P.; de Buhr, Svenja; Hatzopoulos, Georgios N.; Brillard, Charlene; Andany, Santiago; Huebscher, Tania; Sorgenfrei, Frieda A.; Schwarz, Ulrich S.; Graeter, Frauke; Fantner, Georg E.; Goenczy, Pierre
Published in: Nature Communications, Issue 12, 2021, Page(s) 6180, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-26329-1

Seeing and Touching the Mycomembrane at the Nanoscale

Author(s): Albertus Viljoen, Esther Räth, John D. Mckinney, Georg E. Fantner, Yves F. Dufrêne
Published in: Journal of Bacteriology, Issue 203/10, 2021, ISSN 0021-9193
Publisher: American Society for Microbiology
DOI: 10.1128/jb.00547-20

Mechanical Properties of Soft Biological Membranes for Organ-on-a-Chip Assessed by Bulge Test and AFM

Author(s): Pauline Zamprogno, Giuditta Thoma, Veronika Cencen, Dario Ferrari, Barbara Putz, Johann Michler, Georg E. Fantner, Olivier T. Guenat
Published in: ACS Biomaterials Science & Engineering, 2021, ISSN 2373-9878
Publisher: American Chemical Society
DOI: 10.1021/acsbiomaterials.0c00515

Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division

Author(s): Pascal D. Odermatt, Mélanie T. M. Hannebelle, Haig A. Eskandarian, Adrian P. Nievergelt, John D. McKinney, Georg E. Fantner
Published in: Nature Physics, Issue 16/1, 2020, Page(s) 57-62, ISSN 1745-2473
Publisher: Nature Publishing Group
DOI: 10.1038/s41567-019-0679-1

Reducing uncertainties in energy dissipation measurements in atomic force spectroscopy of molecular networks and cell-adhesion studies

Author(s): Soma Biswas, Samuel Leitao, Quentin Theillaud, Blake W. Erickson, Georg E. Fantner
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-26979-0

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Author(s): Adrian P. Nievergelt, Niccolò Banterle, Santiago H. Andany, Pierre Gönczy, Georg E. Fantner
Published in: Nature Nanotechnology, Issue 13/8, 2018, Page(s) 696-701, ISSN 1748-3387
Publisher: Nature Publishing Group
DOI: 10.1038/s41565-018-0149-4

Detecting topological variations of DNA at single-molecule level

Author(s): Ke Liu, Chao Pan, Alexandre Kuhn, Adrian Pascal Nievergelt, Georg E. Fantner, Olgica Milenkovic, Aleksandra Radenovic
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-07924-1

Large‐Range HS‐AFM Imaging of DNA Self‐Assembly through In Situ Data‐Driven Control

Author(s): Adrian P. Nievergelt, Christoph Kammer, Charlène Brillard, Eva Kurisinkal, Maartje M. C. Bastings, Alireza Karimi, Georg E. Fantner
Published in: Small Methods, 2019, Page(s) 1900031, ISSN 2366-9608
Publisher: Wiley
DOI: 10.1002/smtd.201900031

Single-molecule kinetics of pore assembly by the membrane attack complex

Author(s): Edward S. Parsons, George J. Stanley, Alice L. B. Pyne, Adrian W. Hodel, Adrian P. Nievergelt, Anaïs Menny, Alexander R. Yon, Ashlea Rowley, Ralf P. Richter, Georg E. Fantner, Doryen Bubeck, Bart W. Hoogenboom
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-10058-7

High-Throughput Nanocapillary Filling Enabled by Microwave Radiation for Scanning Ion Conductance Microscopy Imaging

Author(s): Vytautas Navikas, Samuel M. Leitão, Sanjin Marion, Sebastian James Davis, Barney Drake, Georg E. Fantner, Aleksandra Radenovic
Published in: ACS Applied Nano Materials, Issue 3/8, 2020, Page(s) 7829-7834, ISSN 2574-0970
Publisher: American chemical society
DOI: 10.1021/acsanm.0c01345

Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface Dynamics

Author(s): Leitao, Samuel M.; Drake, Barney; Pinjusic, Katarina; Pierrat, Xavier; Navikas, Vytautas; Nievergelt, Adrian P.; Brillard, Charlene; Djekic, Denis; Radenovic, Aleksandra; Persat, Alexandre; Constam, Daniel B.; Anders, Jens; Fantner, Georg E.
Published in: Acs Nano, Issue 15, 2022, Page(s) 17613-17622, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.1c05202

A biphasic growth model for cell pole elongation in mycobacteria

Author(s): Mélanie T. M. Hannebelle, Joëlle X. Y. Ven, Chiara Toniolo, Haig A. Eskandarian, Gaëlle Vuaridel-Thurre, John D. McKinney, Georg E. Fantner
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-14088-z

A high-bandwidth voltage amplifier for driving piezoelectric actuators

Author(s): Santiago H. Andany, Adrian P. Nievergelt, Mustafa Kangül, Dominik Ziegler, Georg E. Fantner
Published in: Rev. Sci. Instrum., Issue 94, 093703, 2023, ISSN 0034-6748
Publisher: American Institute of Physics
DOI: 10.1063/5.0159728

Volcano-Shaped Scanning Probe Microscopy Probe for Combined Force-Electrogram Recordings from Excitable Cells

Author(s): B. X. E. Desbiolles, M. T. M Hannebelle, E. de Coulon, A. Bertsch, S. Rohr, G. E. Fantner, P. Renaud
Published in: Nano Letters, Issue 20/6, 2020, Page(s) 4520-4529, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.0c01319

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Author(s): Michael Hohl, Sille Remm, Haig A. Eskandarian, Michael Dal Molin, Fabian M. Arnold, Lea M. Hürlimann, Andri Krügel, Georg E. Fantner, Peter Sander, Markus A. Seeger
Published in: Molecular Microbiology, Issue 111/5, 2019, Page(s) 1263-1282, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14220

Conference proceedings (3)

Data-Driven Feedforward Hysteresis Compensation with Genetic Algorithm for Atomic Force Microscope

Author(s): Asmari, Navid ; Kangul, Mustafa ; Andany, Santiago H. ; Karimi, Alireza ; Fantner, Georg E.
Published in: 2022, ISBN 978-1-6654-5974-7
Publisher: IEEE
DOI: 10.1109/marss55884.2022.9870479

Batch Fabrication of Multilayer Polymer Cantilevers with Integrated Hard Tips for High-Speed Atomic Force Microscopy

Author(s): Nahid Hosseini, Oliver Peric, Matthias Neuenschwander, Santiago H. Andany, Jonathan D. Adams, Georg E. Fantner
Published in: 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII), 2019, Page(s) 2033-2036, ISBN 978-1-5386-8104-6
Publisher: IEEE
DOI: 10.1109/transducers.2019.8808606

Self-Actuated Polymer-Based Cantilevers With Sharp Silicon Tips For High-Speed Atomic Force Microscopy

Author(s): Neuenschwander, Matthias ; Andany, Santiago H. ; Kanguel, Mustafa ; Hosseini, Nahid ; Fantner, Georg E.
Published in: IEEE Xplore, 2021, ISBN 978-1-6654-4845-1
Publisher: IEEE
DOI: 10.1109/transducers50396.2021.9495715

Other (5)

A hybrid polymer/ceramic/semiconductor fabrication platform for high-sensitivity fluid-compatible MEMS devices with sealed integrated electronics

Author(s): Nahid Hosseini, Matthias Neuenschwander, Jonathan D. Adams, Santiago H. Andany, Oliver Peric, Marcel Winhold, Maria Carmen Giordano, Vinayak Shantaram Bhat, Dirk Grundler, Georg E. Fantner
Published in: 2023
Publisher: arXiv
DOI: 10.48550/arxiv.2307.05221

Enhanced feedback performance in off-resonance AFM modes through pulse train sampling

Author(s): Mustafa Kangül, Navid Asmari, Santiago H. Andany, Marcos Penedo, Georg E. Fantner
Published in: 2023
Publisher: arxiv
DOI: 10.48550/arxiv.2306.16013

OpenSIM: open source microscope add-on for structured illumination microscopy

Author(s): Mélanie T.M Hannebelle,Esther Raeth, Samuel M. Leitao, Tomáš Lukeš, Jakub Pospíšil, Chiara Toniolo, Olivier F. Venzin,Antonius Chrisnandy, Prabhu Prasad Swain, Nathan Ronceray, Matthias P. Lütolf, Andrew C. Oates, Guy M. Hagen, Theo Lasser, Aleksandra Radenovic, John D. McKinney, Georg E. Fantner
Published in: 2023
Publisher: BioRxiv
DOI: 10.1101/2023.06.16.545316

Structural flexibility dominates over binding strength for supramolecular crystallinity

Author(s): Vincenzo Caroprese, Cem Tekin, Veronika Cencen, Majid Mosayebi, Tanniemola B. Liverpool, Derek N. Woolfson, Georg Fantner, Maartje M.C. Bastings
Published in: 2023
Publisher: BioRxiv
DOI: 10.1101/2023.09.04.556250

Combining thermal scanning probe lithography and dry etching for grayscale nanopattern amplification

Author(s): Berke Erbas,Ana Conde-Rubio ,Xia Liu ,Joffrey Pernollet ,Zenyu Wang ,Arnaud Bertsch ,Marcos Penedo ,Georg Fantner ,Mitali Banerjee ,Andras Kis ,Giovanni Boero ,Juergen Brugger
Published in: 12 July 2023, Version 1, 2023, ISSN 2573-2293
Publisher: ChemRxiv
DOI: 10.26434/chemrxiv-2023-x0r76

Non-peer reviewed articles (2)

Air and Water-Stable n-Type Doping and Encapsulation of Flexible MoS2 Devices with SU8

Author(s): Kung, Yen-Cheng, Hosseini, Nahid, Dumcenco, Dumitru, Fantner, Georg E.,Kis, Andras
Published in: Advanced Electronic Materials Vol 5, Issue 1, Issue 1800492, 2018, ISSN 2199-160X
Publisher: Wiley-VCH GmbH
DOI: 10.1002/aelm.201800492

Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells

Author(s): Adrian Nievergelt, Charlène Brillard, Haig Eskandarian, John McKinney, Georg Fantner
Published in: International Journal of Molecular Sciences, Issue 19/10, 2018, Page(s) 2984, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms19102984