VIROME NGS ANALYSIS OF PESTS AND PATHOGENS FOR PLANT PROTECTION

Informations projet

VIROPLANT

N° de convention de subvention: 773567

DOI

10.3030/773567

Projet clôturé

Date de signature de la CE 27 Avril 2018

Date de début 1 Mai 2018

Date de fin 31 Octobre 2021

Financé au titre de

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Coût total

€ 3 331 580,00

Contribution de l’UE

€ 3 331 580,00

Coordonné par

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (34)

Collection of new bacterial isolates and phages from Psa, R. solanacearum, Psm, Pss, Agrobacterium biovar 1 and Pph

Collection of new bacterial isolates and phages from Psa R solanacearum Psm Pss Agrobacterium biovar 1 and Pph

Market demand and product requirements
Report on intergenerational surveys about virus-based PPP acceptance

Report on intergenerational surveys about virusbased PPP acceptance

A final report on the possibility of exploitation of VIROPLANT WP outputs
Description of the influence of a mitovirus on the plant performance under biotic and abiotic stresses
Report on the ability of the viral RNA genome to establish an infection in the insect host, and of the infectious clone of EVV1 to replicate in the plant

Report on the ability of the viral RNA genome to establish an infection in the insect host and of the infectious clone of EVV1 to replicate in the plant

Summary of existing regulatory frameworks for bacteriophages and virus-based plant protection products in OECD countries

Summary of existing regulatory frameworks for bacteriophages and virusbased plant protection products in OECD countries

Dissemination/Communication draft plan

The DisseminationCommunication draft plan is a continuous activity and reports will be edited on M3 12 30 41

Final report

Final report on perceived acceptance of virus based PPP based on literature and experts and on intergenerational surveys about virusbased PPP acceptance

Optimized phage cocktail against Psa in kiwi vines, Agrobacterium biovar 1, Psm and Pss

Optimized phage cocktail against Psa in kiwi vines Agrobacterium biovar 1 Psm and Pss

Report on bioinformatic pipeline for virus identification.
Advisory Board Reports

Advisory Board Reports at M19 M42

Report on the importance of microbial community analyses for the environmental risk assessment of plant protection products
Draft report on perceived acceptance of virus based PPP based on literature and experts
First batch of practice abstracts for end-users

"The resulting innovative knowledge and easy accessible end-user material from this project will feed into the EIP-AGRI (The agricultural European Innovation Partnership) website for broad dissemination. The end-user material to be produced contains a substantial number of summaries for practitioners in the EIP common format (""practice abstracts""), including the characteristics of the project (e.g. contact details of partners, etc). A full package of practice abstracts will be produced by the project, containing all the outcomes/recommendations which are ready for practice. A ""practice abstract"" is a short summary of around 1000-1500 characters (word count – no spaces; see below) which describes the main information/recommendation/practice that can serve the end-users in their daily practice. Guidance and templates for these practice abstracts are available on the EIP-AGRI web site: http://ec.europa.eu/eip/agriculture/en/content/eip-agri-common-format. A total target number of 10 practice abstracts is foreseen for the project. Half of them are expected to be delivered in the first batch. "

Case study fact sheets
Report on risk assessment
Second batch of practice abstract for end-users

The resulting innovative knowledge and easy accessible enduser material from this project will feed into the EIPAGRI The agricultural European Innovation Partnership website for broad dissemination The enduser material to be produced contains a substantial number of summaries for practitioners in the EIP common format practice abstracts including the characteristics of the project eg contact details of partners etc A full package of practice abstracts will be produced by the project containing all the outcomesrecommendations which are ready for practiceA practice abstract is a short summary of around 10001500 characters word count no spaces see below which describes the main informationrecommendationpractice that can serve the endusers in their daily practice Guidance and templates for these practice abstracts are available on the EIPAGRI web site httpeceuropaeueipagricultureencontenteipagricommonformat A total target number of 10 practice abstracts is foreseen for the project

Report on bacterial resistance development of Psm and Pss to phage infections
A report on the virome of obligatory biotrophs
Optimized delivery method for Psa phage therapy in kiwi vines
Factors influencing market adoption and strategies to increase likelihood of industrial application
Initial data information packages for approval
Report on the standardization of the methods for identification of viruses from NGS virome analyses of insects
Comprehensive report on technical feasibility of phage therapy for Psa on kiwi vines, halo blight in beans, R. solanacearum in tomato, bacterial canker in Prunus trees and crazy roots in tomato and cucumber

Comprehensive report on technical feasibility of phage therapy for Psa on kiwi vines halo blight in beans R solanacearum in tomato bacterial canker in Prunus trees and crazy roots in tomato and cucumber

Techno-economic system analysis of novel pest control strategies
Comprehensive report of the effect of R. solanacearum phages on the host virulence traits and of their sensitivity to nanoparticles

Comprehensive report of the effect of R solanacearum phages on the host virulence traits and of their sensitivity to nanoparticles

Final data information packages for approval and roadmaps for additional data
Description of the role of a selected insect virus on pests (H. armigera and S. exigua) susceptibility to biological control agents

Description of the role of a selected insect virus on pests H armigera and S exigua susceptibility to biological control agents

Feasible business model configurations for the commercialization of the pest control strategies
Non-virulent P. syringae pathovar as carrier of Psa phage therapy

Nonvirulent P syringae pathovar as carrier of Psa phage therapy

Description of the role of persistent viruses in insect pest/plant interactions

Description of the role of persistent viruses in insect pestplant interactions

Table for the characterization of pathogen transmission parameters of insect vector populations following inoculation with selected viruses
Periodic Activity-Report on Project Dissemination/Communication events

Periodic ActivityReport on Project DisseminationCommunication events three reports will be edited M12 30 42

Websites, patent fillings, videos etc. (1)

Dissemination/Communication contents for different targets

DisseminationCommunication contents for different targets two release of the report will be edited M3 41

Publications

Peer reviewed articles (24)

Putative new plant viruses associated with Plasmopara viticola-infected grapevine samples

Auteurs: Chiapello, Marco; Rodriguez-Romero, Julio; Nerva, Luca; Forgia, Marco; Chitarra, Walter; Ayllon, Maria; Massimo Turina
Publié dans: Annals of applied biology, Numéro 5, 2021, ISSN 1744-7348
Éditeur: Wiley
DOI: 10.5281/zenodo.5810545

Bacteriophage-Host Association in the Phytoplasma Insect Vector Euscelidius variegatus

Auteurs: Marta Vallino, Marika Rossi, Sara Ottati, Gabriele Martino, Luciana Galetto, Cristina Marzachì, Simona Abbà
Publié dans: Pathogens, 2021, ISSN 2076-0817
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pathogens10050612

Characterisation of the Viral Community Associated with the Alfalfa Weevil (Hypera postica) and Its Host Plant, Alfalfa (Medicago sativa)

Auteurs: François, Sarah; Antoine-Lorquin, Aymeric; Kulikowski, Maximilien; Frayssinet, Marie; Filloux, Denis; Fernandez, Emmanuel; Roumagnac, Philippe; Froissart, Remy; Ogliastro, Mylene
Publié dans: Viruses, Numéro 13, 2021, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13050791

Complexity and Local Specificity of the Virome Associated withTospovirus-Transmitting Thrips Species

Auteurs: Chiapello Marco, Bosco Lara, Ciuffo Marina, Ottati Sara, Salem Nida, Rosa Cristina, Tavella Luciana, Turina Massimo
Publié dans: Journal of Virology, 2021, ISSN 1098-5514
Éditeur: ASM
DOI: 10.1128/jvi.00597-21

Preparing for the KIL: Receptor Analysis of Pseudomonas syringae Pv. porri Phages and Their Impact on Bacterial Virulence

Auteurs: Dominique Holtappels, Alison Kerremans, Yoni Busschots, Johan Van Vaerenbergh, Martine Maes, Rob Lavigne, Jeroen Wagemans
Publié dans: International Journal of Molecular Science, 2020, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21082930

Silencing of ATP synthase β reduces phytoplasma multiplication in a leafhopper vector

Auteurs: Galetto, Luciana; Abbà, Simona; Rossi, Marika; Ripamonti, Matteo; Palmano, Sabrina; Bosco, Domenico; Marzachì, Cristina
Publié dans: Journal of Insect Physiology, Numéro 128, 2020, ISSN 0022-1910
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.jinsphys.2020.104176

Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus

Auteurs: Ana Ruiz-Padilla, Julio Rodríguez-Romero, Irene Gómez-Cid, Davide Pacifico, María A Ayllón
Publié dans: mBIO, 2021, ISSN 2150-7511
Éditeur: ASM
DOI: 10.1128/mbio.03705-20

Seeing the forest for the trees: Use of phages to treat bacterial tree diseases.

Auteurs: Emily R. Grace, Mojgan Rabiey, Ville-Petri Friman, Robert W. Jackson
Publié dans: Plant Pathology, 2021, ISSN 1365-3059
Éditeur: British Society for Plant Pathology
DOI: 10.1111/ppa.13465

Viruses of fungi and oomycetes in the soil environment

Auteurs: Sutela S., Poimala A., Vainio E. J.
Publié dans: FEMS Microbiology Ecology, 2019, ISSN 0168-6496
Éditeur: Blackwell
DOI: 10.1093/femsec/fiz119

Phage biocontrol to combat Pseudomonas syringae pathogens causing disease in cherry

Auteurs: Mojgan Rabiey, Shyamali R. Roy, Dominique Holtappels, Linda Franceschetti, Billy J. Quilty, Ryan Creeth, George W. Sundin, Jeroen Wagemans, Rob Lavigne, Robert W. Jackson
Publié dans: Microbial Biotechnology, 2020, ISSN 1751-7915
Éditeur: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13585

Influence of Diet, Sex, and Viral Infections on the Gut Microbiota Composition of Spodoptera exigua Caterpillars

Auteurs: Martínez Solís, María; Collado, María Carmen; Herrero, Salvador
Publié dans: Frontiers in Microbiology, Vol 11 (2020), Numéro 12, 2020, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2020.00753

Analysis of the virome associated to grapevine downy mildew lesions reveals new mycovirus lineages

Auteurs: M Chiapello, J Rodríguez-Romero, M A Ayllón, M Turina
Publié dans: Virus Evolution, 2020, ISSN 2057-1577
Éditeur: Oxford Academic
DOI: 10.1093/ve/veaa058

Molecular Characterization and Taxonomic Assignment of Three Phage Isolates from a Collection Infecting Pseudomonas syringae pv. actinidiae and P. syringae pv. phaseolicola from Northern Italy

Auteurs: Martino, Gabriele; Holtappels, Dominique; Vallino, Marta; Chiapello, Marco; Turina Massimo; Lavigne, Rob; Wagemans, Jeroen; Ciuffo, Marina
Publié dans: Viruses, Numéro 1, 2021, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13102083

Presence of a Mitovirus Is Associated with Alteration of theMitochondrial Proteome, as Revealed by Protein-ProteinInteraction (PPI) and Co-Expression Network Models inChenopodium quinoa Plants

Auteurs: Di Silvestre, D.; Passignani, G.; Rossi, R.; Ciuffo, M.; Turina, M.; Vigani, G.; Mauri, P.
Publié dans: Biology, 2022, ISSN 2079-7737
Éditeur: MDPI
DOI: 10.3390/biology11010095

Biological characterization of Euscelidius variegatus iflavirus 1

Auteurs: Sara Ottati; Alberto Persico; Marika Rossi; Domenico Bosco; Marta Vallino; Simona Abbà; Giulia Molinatto; Sabrina Palmano; Raffaella Balestrini; Luciana Galetto; Cristina Marzachì
Publié dans: Journal of Invertebrate Pathology, Numéro 5, 2020, ISSN 0022-2011
Éditeur: Academic Press
DOI: 10.1101/2020.03.31.018010

A New Prevalent Densovirus Discovered in Acari. Insight from Metagenomics in Viral Communities Associated with Two-Spotted Mite (Tetranychus urticae) Populations

Auteurs: Sarah François, Doriane Mutuel, Alison Duncan, Leonor Rodrigues, Celya Danzelle, Sophie Lefevre, Inês Santos, Marie Frayssinet, Emmanuel Fernandez, Denis Filloux, Philippe Roumagnac, Rémy Froissart, Mylène Ogliastro
Publié dans: Viruses, Numéro 11/3, 2019, Page(s) 233, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v11030233

Protection of Phage Applications in Crop Production: A Patent Landscape

Auteurs: Dominique Holtappels, Rob Lavigne, Isabelle Huys, Jeroen Wagemans
Publié dans: Viruses, Numéro 11/3, 2019, Page(s) 277, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v11030277

Biological and Molecular Characterization of Chenopodium quinoa Mitovirus 1 Reveals a Distinct Small RNA Response Compared to Those of Cytoplasmic RNA Viruses

Auteurs: L. Nerva, G. Vigani, D. Di Silvestre, M. Ciuffo, M. Forgia, W. Chitarra, M. Turina
Publié dans: Journal of Virology, Numéro 93/7, 2019, ISSN 0022-538X
Éditeur: American Society for Microbiology
DOI: 10.1128/jvi.01998-18

Making Viruses More Socially Acceptable: A Sociological Framework For Virus-based Plant Protection Products

Auteurs: Carradore, R
Publié dans: TEORIJA IN PRAKSA, Numéro 1, 2019, ISSN 0040-3598
Éditeur: Univerza Edvarda Kardelja v Ljubljani, Fakulteta za Sociologija Politicne Vede in Novinarstvo

"New viral sequences identified in the flavescence dorée phytoplasma vector scaphoideus titanus"

Auteurs: Ottati, S.; Chiapello, M.; Galetto, L.; Bosco, D.; Marzachi, C.; Abba, S.
Publié dans: Viruses, Numéro 2, 2020, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12030287

Viral diversity in Phytophthora cactorum population infecting strawberry

Auteurs: Anna Poimala, Päivi Parikka, Jarkko Hantula, Eeva J. Vainio
Publié dans: Environmental Microbiology, Numéro 23, 2021, ISSN 1462-2920
Éditeur: Wiley
DOI: 10.1111/1462-2920.15519

Viroplant in a Nutshell

Auteurs: Rob Lavigne, Massimo Turina, Jeroen Wagemans
Publié dans: Phage, 2020, ISSN 2641-6530
Éditeur: Mary Ann Liebert, Inc
DOI: 10.1089/phage.2020.0045

Silencing of ATP Synthase β Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Dorée

Auteurs: Matteo Ripamonti, Luca Cerone, Simona Abbà, Marika Rossi, Sara Ottati, Sabrina Palmano, Cristina Marzachì and Luciana Galetto
Publié dans: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23020765

Complete genome sequence of a novel toti-like virus from the plant-pathogenic oomycete Phytophthora cactorum.

Auteurs: Anna Poimala; Eeva J. Vainio
Publié dans: Archives of Virology, Numéro 7, 2020, ISSN 0304-8608
Éditeur: Springer Verlag
DOI: 10.1007/s00705-020-04642-2