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Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit

Deliverables

e-Forum for exchanges with end-users and policy makers

e-Forum for exchanges with end-users and policy makers

Project website

Project website where information about the project and the progress will be available

Report on communication activities

Communication events workshops or webinars video leaflets publications flyers press releases papers etc will be a high priority for SuperPests The format of information to the various stakeholders will be tailored towards the specific needs of the various target groups which will include the academic community agricultural authorities and policy makers at local and national levels local stakeholder advisory groups and endusers nongovernmental organizations and civil society Appropriate communications will be developed and provided to each of the stakeholder groups Peer reviewed articles and presentations at learned meetings and international conferences will be published whereas for research subjects the information provided will for example include brochures and posters for community engagement and eliciting individual informed assentconsent Project activities will be collectively reported in an annual newsletter designed for general readership and provided in both French and English The coordinator will set up a website with passwordprotected intranet during the first months of the project which will be maintained and updated throughout the project lifetime

Practice abstracts for major pests, resistance management tools and new biopesticides

The resulting innovative knowledge and easy accessible enduser material from this project will feed into the EIPAGRI The agricultural European Innovation Partnership website for broad dissemination The enduser material to be produced contains a substantial number of summaries for practitioners in the EIP common format practice abstracts including the characteristics of the project eg contact details of partners etc A full package of practice abstracts will be produced by the project containing all the outcomesrecommendations which are ready for practiceA practice abstract is a short summary of around 10001500 characters word count no spaces see below which describes the main informationrecommendationpractice that can serve the endusers in their daily practice Guidance and templates for these practice abstracts are available on the EIPAGRI web site httpeceuropaeueipagricultureencontenteipagricommonformat A total target number of 4 practice abstracts is foreseen for the project Half of them are expected to be delivered in the first batch Practice Abstracts 1 Major pests and resistance management and tools including diagnostic 2 New biopesticides

Report on host-plant factors determining the ability of A. swirskii and O. levigatus to display sufficient population growth

Report on hostplant factors determining the ability of A swirskii and O levigatus to display sufficient population growth

Report for available screening platforms and pipelines

The integrated and fully automated diagnostic platform will be validated against a number of different samples from several geographical regions in comparison to existing gold standard assays This concept has a high probability of success because the disc cartridge has been tested in other systems eg human diagnostics It is robust highly sensitive and specific and can be very adaptable to diagnostic needs in terms of the nature and number of detectable species including applications related to vector control ie wwwdmcmalveceu The LabDisk will offer higher added value compared to performing individual assays as it will also include markers for metabolic resistance and provide sufficient information for operational level decision making at an affordable cost

Manuscripts on functional characterisation of resistance (target: >2)

The results will be published in scientific journals and conferences

Manuscript on the salivary effector proteins of whiteflies.

Collect and sequence salivary proteome from B tabaci on artificial diet mapping references are available select putative effector candidates transiently overexpress top 10 candidates in tobacco and assess the impact on whitefly performance Select the best effector candidate

Report of the Kick-off meeting

Information about the Kick off meeting where all the partners will meet to make the plan of action

LabDisk specifications document and assay protocols

The novel molecular markers that will be included to the LabDisk in order to have fast and reliable results with low cost

Novel molecular markers for emerging resistance cases (target: >5)

We will investigate field populations sampled from various economically important crops that are reported by farmers and extension services to be particularly difficult to control with current chemical strategies, implying the evolution of resistance. We will prioritise cases of potent and novel resistance (i.e. chemistry that has not been previously compromised by resistance). As a consortium we are part of, or have access to, extensive monitoring networks, which will provide access to these resistant populations. The following compounds/pests will be included in the analysis: tetronic acids (keto-enols) – spirodiclofen, spiromesifen and spirotetramat / T. urticae, B. tabaci and M. persicae; pyflubumide, cyenopyrafen and cyflumetofen (most recently developed acaricides acting on Complex II of the mitochondrial electron transport chain, recently introduced to the market) / T. urticae; abamectin (a neurotoxic insecticide/acaricide of very low toxicity to mammals) / F. occidentalis, T. urticae; spinosad (a low toxicity insecticide of natural origin) / F. occidentalis; pymetrozine/flonicamid / B. tabaci and M. persicae.

Report of metabolic liability and anti-resistance potential of bio-pesticides

Report of metabolic liability and antiresistance potential of biopesticides

Review of biopesticide pest control options and regulatory pathways

Review of biopesticide pest control options and regulatory pathways

Workshops or webinars for biopesticides (report)

Workshops or webinars for biopesticides report

Pedagogic supports and e-learning units for training in Crop Protection based on SuperPests outcomes

Pedagogic supports and elearning units for training in Crop Protection based on SuperPests outcomes

Kits, lyophilised components and disc cartridges manufactured

The molecular identification of the selected targets will be done via appropriate amplification assays using lyophilized mastermix and suitable primers The portable instrument will include an optical unit detector Kits consisting of lyophilized components and disc cartridges will be assembled and packaged by ALUFR

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Publications

Challenges facing arthropod biological control: identifying traits for genetic improvement of predators in protected crops

Author(s): Pablo Bielza, Virginia Balanza, Dina Cifuentes, José E Mendoza
Published in: Pest Management Science, 76/11, 2020, Page(s) 3517-3526, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.5857

Adaptive divergence and post-zygotic barriers to gene flow between sympatric populations of a herbivorous mite

Author(s): Ernesto Villacis-Perez, Simon Snoeck, Andre H. Kurlovs, Richard M. Clark, Johannes A. J. Breeuwer, Thomas Van Leeuwen
Published in: Communications Biology, 4/1, 2021, ISSN 2399-3642
Publisher: Nature Research
DOI: 10.1038/s42003-021-02380-y

Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s

Author(s): Amy McLeman, Bartlomiej J. Troczka, Rafael A. Homem, Ana Duarte, Christoph Zimmer, William T. Garrood, Adam Pym, Katherine Beadle, Rebecca J. Reid, Vassilis Douris, John Vontas, T.G. Emyr Davies, Richard ffrench Constant, Ralf Nauen, Chris Bass
Published in: Pesticide Biochemistry and Physiology, 169, 2020, Page(s) 104674, ISSN 0048-3575
Publisher: Academic Press
DOI: 10.1016/j.pestbp.2020.104674

Diversity and evolution of the P450 family in arthropods

Author(s): Wannes Dermauw, Thomas Van Leeuwen, René Feyereisen
Published in: Insect Biochemistry and Molecular Biology, 127, 2020, Page(s) 103490, ISSN 0965-1748
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.ibmb.2020.103490

Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae

Author(s): Michela Panini, Olga Chiesa, Bartlomiej J. Troczka, Mark Mallott, Gian Carlo Manicardi, Stefano Cassanelli, Filippo Cominelli, Alex Hayward, Emanuele Mazzoni, Chris Bass
Published in: Proceedings of the National Academy of Sciences, 118/23, 2021, Page(s) e2100559118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2100559118

Significance and interpretation of molecular diagnostics for insecticide resistance management of agricultural pests

Author(s): Thomas Van Leeuwen, Wannes Dermauw, Konstantinos Mavridis, John Vontas
Published in: Current Opinion in Insect Science, 39, 2020, Page(s) 69-76, ISSN 2214-5745
Publisher: Elsevier BV
DOI: 10.1016/j.cois.2020.03.006

Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci

Author(s): Bettina Lueke, Vassilis Douris, Jamie E. Hopkinson, Frank Maiwald, Gillian Hertlein, Kyriaki-Maria Papapostolou, Pablo Bielza, Anastasia Tsagkarakou, Thomas Van Leeuwen, Chris Bass, John Vontas, Ralf Nauen
Published in: Pesticide Biochemistry and Physiology, 166, 2020, Page(s) 104583, ISSN 0048-3575
Publisher: Academic Press
DOI: 10.1016/j.pestbp.2020.104583

Life stage-dependent genetic traits as drivers of plant–herbivore interactions

Author(s): Merijn R Kant, Robert C Schuurink
Published in: Current Opinion in Biotechnology, 70, 2021, Page(s) 234-240, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2021.06.012

Spider Mites Cause More Damage to Tomato in the Dark When Induced Defenses Are Lower

Author(s): Jie Liu, Rachid Chafi, Saioa Legarrea, Juan M. Alba, Tomas Meijer, Steph B. J. Menken, and Merijn R. Kant
Published in: Journal of Chemical Ecology, 2020, ISSN 0098-0331
Publisher: Kluwer Academic/Plenum Publishers
DOI: 10.1007/s10886-020-01195-1

Metabolic mechanisms of resistance to spirodiclofen and spiromesifen in Iranian populations of Panonychus ulmi

Author(s): Fahimeh Badieinia, Jahangir Khajehali, Ralf Nauen, Wannes Dermauw, Thomas Van Leeuwen
Published in: Crop Protection, 134, 2020, Page(s) 105166, ISSN 0261-2194
Publisher: Butterworth Scientific Ltd.
DOI: 10.1016/j.cropro.2020.105166

Using CRISPR/Cas9 genome modification to understand the genetic basis of insecticide resistance: Drosophila and beyond

Author(s): Vassilis Douris, Shane Denecke, Thomas Van Leeuwen, Chris Bass, Ralf Nauen, John Vontas
Published in: Pesticide Biochemistry and Physiology, 167, 2020, Page(s) 104595, ISSN 0048-3575
Publisher: Academic Press
DOI: 10.1016/j.pestbp.2020.104595

Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae

Author(s): Seyedeh Masoumeh Fotoukkiaii, Catherine Mermans, Nicky Wybouw, Thomas Van Leeuwen
Published in: Journal of Pest Science, 93/3, 2020, Page(s) 1085-1096, ISSN 1612-4758
Publisher: Springer Verlag
DOI: 10.1007/s10340-020-01213-x

Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

Author(s): Kyriaki Maria Papapostolou, Maria Riga, Jason Charamis, Evangelia Skoufa, Vassilis Souchlas, Aris Ilias, Wannes Dermauw, Panagiotis Ioannidis, Thomas Van Leeuwen, John Vontas
Published in: Pest Management Science, 77/2, 2021, Page(s) 666-676, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.6136

Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

Author(s): Nicolas Bensoussan, Sameer Dixit, Midori Tabara, David Letwin, Maja Milojevic, Michele Antonacci, Pengyu Jin, Yuka Arai, Kristie Bruinsma, Takeshi Suzuki, Toshiyuki Fukuhara, Vladimir Zhurov, Sven Geibel, Ralf Nauen, Miodrag Grbic, Vojislava Grbic
Published in: Scientific Reports, 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-75682-6

Genome streamlining in a minute herbivore that manipulates its host plant

Author(s): Robert Greenhalgh, Wannes Dermauw, Joris J Glas, Stephane Rombauts, Nicky Wybouw, Jainy Thomas, Juan M Alba, Ellen J Pritham, Saioa Legarrea, René Feyereisen, Yves Van de Peer, Thomas Van Leeuwen, Richard M Clark, Merijn R Kant
Published in: eLife, 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.56689

Is the emerging mite pest Aculops lycopersici controllable? Global and genome‐based insights in its biology and management

Author(s): Lore Vervaet, Raf De Vis, Patrick De Clercq, Thomas Van Leeuwen
Published in: Pest Management Science, 2020, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.6265

High-resolution genetic mapping reveals cis-regulatory and copy number variation in loci associated with cytochrome P450-mediated detoxification in a generalist arthropod pest

Author(s): Seyedeh Masoumeh Fotoukkiaii, Nicky Wybouw, Andre H. Kurlovs, Dimitra Tsakireli, Spiros A. Pergantis, Richard M. Clark, John Vontas, Thomas Van Leeuwen
Published in: PLOS Genetics, 17/6, 2021, Page(s) e1009422, ISSN 1553-7404
Publisher: PLOS
DOI: 10.1371/journal.pgen.1009422

Untangling a G ordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

Author(s): Wenxin Xue, Catherine Mermans, Kyriaki‐Maria Papapostolou, Mantha Lamprousi, Iason‐Konstantinos Christou, Emre Inak, Vassilis Douris, John Vontas, Wannes Dermauw, Thomas Van Leeuwen
Published in: Pest Management Science, 2020, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.6215

‘What I cannot create, I do not understand’: functionally validated synergism of metabolic and target site insecticide resistance

Author(s): George-Rafael Samantsidis, Rafaela Panteleri, Shane Denecke, Stella Kounadi, Iason Christou, Ralf Nauen, Vassilis Douris, John Vontas
Published in: Proceedings of the Royal Society B: Biological Sciences, 287/1927, 2020, Page(s) 20200838, ISSN 0962-8452
Publisher: Royal Society of London
DOI: 10.1098/rspb.2020.0838

Improving the compatibility of pesticides and predatory mites: recent findings on physiological and ecological selectivity

Author(s): Carlo Duso, Thomas Van Leeuwen, Alberto Pozzebon
Published in: Current Opinion in Insect Science, 39, 2020, Page(s) 63-68, ISSN 2214-5745
Publisher: Elsevier BV
DOI: 10.1016/j.cois.2020.03.005

Substrate specificity and promiscuity of horizontally transferred UDP-glycosyltransferases in the generalist herbivore Tetranychus urticae

Author(s): Simon Snoeck, Nena Pavlidi, Dimitra Pipini, John Vontas, Wannes Dermauw, Thomas Van Leeuwen
Published in: Insect Biochemistry and Molecular Biology, 109, 2019, Page(s) 116-127, ISSN 0965-1748
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.ibmb.2019.04.010

Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri

Author(s): Elaheh Shafiei Alavijeh, Jahangir Khajehali, Simon Snoeck, Rafaela Panteleri, Mohammad Ghadamyari, Wim Jonckheere, Sabina Bajda, Corinna Saalwaechter, Sven Geibel, Vassilis Douris, John Vontas, Thomas Van Leeuwen, Wannes Dermauw
Published in: Pesticide Biochemistry and Physiology, 2019, ISSN 0048-3575
Publisher: Academic Press
DOI: 10.1016/j.pestbp.2019.12.009

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae

Author(s): Wannes Dermauw, Wim Jonckheere, Maria Riga, Ioannis Livadaras, John Vontas, Thomas Van Leeuwen
Published in: Insect Biochemistry and Molecular Biology, 120, 2020, Page(s) 103347, ISSN 0965-1748
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.ibmb.2020.103347

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Author(s): Emre İnak, Yasin Nazım Alpkent, Sultan Çobanoğlu, Wannes Dermauw, Thomas Van Leeuwen
Published in: Experimental and Applied Acarology, 78/3, 2019, Page(s) 343-360, ISSN 0168-8162
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10493-019-00398-w

Convergent evolution of cytochrome P450s underlies independent origins of keto-carotenoid pigmentation in animals

Author(s): Nicky Wybouw, Andre H. Kurlovs, Robert Greenhalgh, Astrid Bryon, Olivia Kosterlitz, Yuki Manabe, Masahiro Osakabe, John Vontas, Richard M. Clark, Thomas Van Leeuwen
Published in: Proceedings of the Royal Society B: Biological Sciences, 286/1907, 2019, Page(s) 20191039, ISSN 0962-8452
Publisher: Royal Society of London
DOI: 10.1098/rspb.2019.1039

High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides

Author(s): Simon Snoeck, Andre H. Kurlovs, Sabina Bajda, René Feyereisen, Robert Greenhalgh, Ernesto Villacis-Perez, Olivia Kosterlitz, Wannes Dermauw, Richard M. Clark, Thomas Van Leeuwen
Published in: Insect Biochemistry and Molecular Biology, 110, 2019, Page(s) 19-33, ISSN 0965-1748
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.ibmb.2019.04.011

Identification and geographical distribution of pyrethroid resistance mutations in the poultry red mite Dermanyssus gallinae

Author(s): Evangelia Katsavou, Spyros Vlogiannitis, Eleanor Karp‐Tatham, Damer P Blake, Aris Ilias, Christina Strube, Ilias Kioulos, Wannes Dermauw, Thomas Van Leeuwen, John Vontas
Published in: Pest Management Science, 76/1, 2019, Page(s) 125-133, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.5582

Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen

Author(s): Peng Wei, Peter Demaeght, Kristof De Schutter, Linda Grigoraki, Vassiliki Labropoulou, Maria Riga, John Vontas, Ralf Nauen, Wannes Dermauw, Thomas Van Leeuwen
Published in: Pest Management Science, 76/3, 2019, Page(s) 1142-1153, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.5627

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider mite Tetranychus urticae

Author(s): Seyedeh Masoumeh Fotoukkiaii, Zoë Tan, Wenxin Xue, Nicky Wybouw, Thomas Van Leeuwen
Published in: Pest Management Science, 76/3, 2019, Page(s) 1154-1163, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.5628

Trait mapping in diverse arthropods by bulked segregant analysis

Author(s): Andre H Kurlovs, Simon Snoeck, Olivia Kosterlitz, Thomas Van Leeuwen, Richard M Clark
Published in: Current Opinion in Insect Science, 36, 2019, Page(s) 57-65, ISSN 2214-5745
Publisher: Elsevier BV
DOI: 10.1016/j.cois.2019.08.004

Characterization of abamectin resistance in Iranian populations of European red mite, Panonychus ulmi Koch (Acari: Tetranychidae)

Author(s): Fariborz Rameshgar, Jahangir Khajehali, Ralf Nauen, Wannes Dermauw, Thomas Van Leeuwen
Published in: Crop Protection, 125, 2019, Page(s) 104903, ISSN 0261-2194
Publisher: Butterworth Scientific Ltd.
DOI: 10.1016/j.cropro.2019.104903

Spotlight on the Roles of Whitefly Effectors in Insect–Plant Interactions

Author(s): Diana Naalden, Paula J. M. van Kleeff, Sarmina Dangol, Marieke Mastop, Rebecca Corkill, Saskia A. Hogenhout, Merijn R. Kant, Robert C. Schuurink
Published in: Frontiers in Plant Science, 12, 2021, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2021.661141

Food web engineering: ecology and evolution to improve biological pest control

Author(s): Marta Montserrat, Diego Serrano-Carnero, Inmaculada Torres-Campos, Mehdi Bohloolzadeh, Dolores Ruiz-Lupión, Jordi Moya-Laraño
Published in: Current Opinion in Insect Science, 47, 2021, Page(s) 125-135, ISSN 2214-5745
Publisher: Elsevier BV
DOI: 10.1016/j.cois.2021.06.006

Short term transcriptional responses of P450s to phytochemicals in insects and mites

Author(s): Marilou Vandenhole, Wannes Dermauw, Thomas Van Leeuwen
Published in: Current Opinion in Insect Science, 43, 2021, Page(s) 117-127, ISSN 2214-5745
Publisher: Elsevier BV
DOI: 10.1016/j.cois.2020.12.002

Geographical distribution and molecular insights into abamectin and milbemectin cross‐resistance in European field populations of Tetranychus urticae

Author(s): Wenxin Xue, Simon Snoeck, Christine Njiru, Emre Inak, Wannes Dermauw, Thomas Van Leeuwen
Published in: Pest Management Science, 76/8, 2020, Page(s) 2569-2581, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.5831

Juvenile Spider Mites Induce Salicylate Defenses, but Not Jasmonate Defenses, Unlike Adults

Author(s): Jie Liu, Saioa Legarrea, Juan M. Alba, Lin Dong, Rachid Chafi, Steph B. J. Menken, Merijn R. Kant
Published in: Frontiers in Plant Science, 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.00980