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Novel 1D photonic metal oxide nanostructures for early stage cancer detection

Periodic Reporting for period 1 - CanBioSe (Novel 1D photonic metal oxide nanostructures for early stage cancer detection)

Reporting period: 2018-01-01 to 2019-12-31

The CanBioSe project research and innovation goals are targeted to develop a new portable tool for early stage cancer detection which can solve one of the important health challenges in EU society. The project is targeted to strengthen international and intersectoral collaboration, sharing new ideas, knowledge transfer from research to market, and vice versa in the field of nanostructured metal oxide optical biosensors for cancer cell detection.

The project partners will provide research and training activities in the fields of nanotechnology, surface functionalization, bioengineering, microfluidics and biosensor testing, market analysis and commercialisation. The provided research and management training to experienced researchers and early stage researchers will be quite beneficial. It will strengthen their personal skills and CVs via the new scientific papers and conference theses and strengthen a development of EU research human resources. We foresee a long lasting collaboration between the partners, based on co-supervising students and the preparation of novel collaborative project proposals. Also foreseen is a dissemination of the project results to the scientific society and wide auditories.

Scientific work will include: One dimensional (1D) polimer nanofibers will be deposited by an electrospinning technique. Photonic nanomaterials, based on metal oxide based nanostructures (ZnO, ZnO/Al2O3 nanolaminates, Au/ZnO and ZnO/Au) will coat the 1D nanofibers. Metal oxides and Au nanoparticles will be deposited with Atomic Layer Deposition (ALD) and electrophoresis, respectively. Bioselective layer will be formed by immobilization of specific antibodies on the biosensor surface. Photoluminescence and optical spectroscopy will be used for recording the biosensor signal. Biosensor testing will be performed on cancer cells (human chronic lymphocyte leukemia (CLL) leucosis and acute lymphoblastic leucosis). The biosensor will be integrated with microfluidic system in order to minimize dimensions and simplify the use of the detection system.
During the first year of the CanBioSe project the scientific work performed and main results achieved are:

There have been PAN, HA, collagen and gelatin fibers formed with tailored properties, ZnO nanolayers with different thickness deposited on PAN nanofiber scaffolds. Structure properties of ZnO by XRD, SEM and TEM have been investigated (published). Optical properties of ZnO have been investigated (presented at workshop, ready for publishing).
Experimental chamber for photoluminescence measurements of sensor signal in liquid has been developed and tested. The chamber was assembled with fluidic system using syringe pump. The system can be assembled with lasers and LEDs.

The project partners during the first year of the project have been providing training in following disciplines:
Riga team: Reflectance and photoluminescence spectroscopy for materials characterization and biosensor testing, cell growing and biomanipulation;
Vilnius team: FTIR and Raman spectroscopy for characterization of materials and biosensor testing, biofunctionalization of the nanostructured surface;
Poznan Team: TEM and XPS/UPS spectroscopy for surface analysis of the nanostructures;
Minsk team: cell culture growth, biomanipulation, fluorescence microscopy of bioprobes cells, separation of cell subpopulations by flow cytometry.
Progress beyond the state of the art:
Novel 1D ZnO nanostructures with advanced structure and optical properties have been achieved. Through tailoring of deposition parameters, optical signal (photoluminescence) is much stronger than other materials, reported in literature. Fundamental properties of these nanostructures have been investigated.

Expected results until the end of the project:
1D metal oxide nanostructures with tailored properties as platforms for optical biosensors. Formed bioselective layer with high stability and selectivity on the surface of 1D metal oxide nanostructures. Sensitivity and kinetics of the 1D photonic biosensors to different concentrations of target molecules (cellular receptors and molecular markers of cancer cells). Mechanisms of interaction between biomolecules and the biosensor surface. Developed portable biosensor system for cancer detection, integrated with microfluidic flow and optical fiber detection systems.

Potential impact
The impact of the project is following:
1.Socio-economic impact - the project will develop new methodology for early stage cancer detection, and that will impact the health care of EU citizens. Development of novel integration devices will impact the EU industry. New results will stimulate development of EU research in fields of nano- and biotechnology.
2.Career development and strengthening of research potential - new skills will be transferred to early stage researchers in order to improve their competitiveness. Effective knowledge transfer increases scientific potential of participating countries. New proposed projects stimulate staff exchange, new papers and applications.
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