Molecular mechanisms, evolutionary impacts and applications of prokaryotic epigenetic-targeted immune systems

Project Information

EPICut

Grant agreement ID: 788405

DOI 10.3030/788405

EC signature date 8 June 2018

Start date 1 August 2018

End date 31 July 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 196 413,75

EU contribution € 2 196 413,75

Coordinated by

UNIVERSITY OF BRISTOL
United Kingdom

Publications

Peer reviewed articles (5)

ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing

Author(s): Oscar E Torres Montaguth, Stephen J Cross, Kincaid W A Ingram, Laura Lee, Fiona M Diffin, Mark D Szczelkun
Published in: Nucleic Acids Research, 2021, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab727

Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition

Author(s): Tatiana Dimitriu, Elena Kurilovich, Urszula Łapińska, Konstantin Severinov, Stefano Pagliara, Mark D Szczelkun, Edze R Westra
Published in: Cell Host Microbe, 2021, ISSN 1931-3128
Publisher: Cell Press
DOI: 10.1016/j.chom.2021.11.014

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

Author(s): Grace Mullally, Kara van Aelst, Mohsin M Naqvi, Fiona M Diffin, Tautvydas Karvelis, Giedrius Gasiunas, Virginijus Siksnys, Mark D Szczelkun
Published in: Nucleic Acids Research, Issue 48/12, 2020, Page(s) 6811-6823, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa477

CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavage

Author(s): Mohsin M. Naqvi, Laura Lee, Oscar E. Torres Montaguth, Fiona M. Diffin, Mark D. Szczelkun
Published in: Nature Chemical Biology, Issue 18, 2022, Page(s) 1014–1022, ISSN 1552-4469
Publisher: Springer Nature
DOI: 10.1038/s41589-022-01082-8

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

Author(s): Kara van Aelst, Carlos Martínez-Santiago, Stephen Cross, Mark Szczelkun
Published in: Genes, Issue 10/2, 2019, Page(s) 169, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10020169

Non-peer reviewed articles (1)

Evolutionary Ecology and Interplay of Prokaryotic Innate and Adaptive Immune Systems

Author(s): Tatiana Dimitriu, Mark D. Szczelkun, Edze R. Westra
Published in: Current Biology, Issue 30/19, 2020, Page(s) R1189-R1202, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2020.08.028