Molecular mechanisms, evolutionary impacts and applications of prokaryotic epigenetic-targeted immune systems

Project Information

EPICut

Grant agreement ID: 788405

DOI 10.3030/788405

Project closed

EC signature date 8 June 2018

Start date 1 August 2018

End date 31 July 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 196 413,75

EU contribution € 2 196 413,75

Coordinated by

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (8)

ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing

Author(s): Oscar E Torres Montaguth, Stephen J Cross, Kincaid W A Ingram, Laura Lee, Fiona M Diffin, Mark D Szczelkun
Published in: Nucleic Acids Research, 2021, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab727

Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition

Author(s): Tatiana Dimitriu, Elena Kurilovich, Urszula Łapińska, Konstantin Severinov, Stefano Pagliara, Mark D Szczelkun, Edze R Westra
Published in: Cell Host Microbe, 2021, ISSN 1931-3128
Publisher: Cell Press
DOI: 10.1016/j.chom.2021.11.014

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

Author(s): Grace Mullally, Kara van Aelst, Mohsin M Naqvi, Fiona M Diffin, Tautvydas Karvelis, Giedrius Gasiunas, Virginijus Siksnys, Mark D Szczelkun
Published in: Nucleic Acids Research, Issue 48/12, 2020, Page(s) 6811-6823, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa477

CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavage

Author(s): Mohsin M. Naqvi, Laura Lee, Oscar E. Torres Montaguth, Fiona M. Diffin, Mark D. Szczelkun
Published in: Nature Chemical Biology, Issue 18, 2022, Page(s) 1014–1022, ISSN 1552-4469
Publisher: Springer Nature
DOI: 10.1038/s41589-022-01082-8

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

Author(s): Kara van Aelst, Carlos Martínez-Santiago, Stephen Cross, Mark Szczelkun
Published in: Genes, Issue 10/2, 2019, Page(s) 169, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10020169

Hexameric assembly of the AAA+ protein McrB is necessary for GTPase activity

Author(s): Neha Nirwan, Pratima Singh, Gyana Gourab Mishra, Christopher M Johnson, Mark D Szczelkun, Katsuaki Inoue, Kutti R Vinothkumar, Kayarat Saikrishnan
Published in: Nucleic Acids Research, Issue 47, 2022, Page(s) 868-882, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gky1170

The bacterial defense system MADS interacts with CRISPR-Cas to limit phage infection and escape

Author(s): Alice Maestri, Benoit J. Pons, Elizabeth Pursey, Charlotte E. Chong, Sylvain Gandon, Rafael Custodio, Anna Olina, Aleksei Agapov, Matthew A.W. Chisnall, Anita Grasso, Steve Paterson, Mark D. Szczelkun, Kate S. Baker, Stineke van Houte, Anne Chevallereau, Edze R. Westra
Published in: Cell Host & Microbe, 2024, ISSN 1931-3128
Publisher: Cell Press
DOI: 10.1016/j.chom.2024.07.005

Short-range translocation by a restriction enzyme motor triggers diffusion along DNA

Author(s): Martin Göse, Emma E. Magill, Alex Hughes-Games, Steven J. Shaw, Fiona M. Diffin, Tara Rawson, Zsofia Nagy, Ralf Seidel, Mark D. Szczelkun
Published in: Nature Chemical Biology, Issue 20, 2024, Page(s) 689-698, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-023-01504-1

Non-peer reviewed articles (1)

Evolutionary Ecology and Interplay of Prokaryotic Innate and Adaptive Immune Systems

Author(s): Tatiana Dimitriu, Mark D. Szczelkun, Edze R. Westra
Published in: Current Biology, Issue 30/19, 2020, Page(s) R1189-R1202, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2020.08.028

Other (1)

Various plasmid strategies limit the effect of bacterial Restriction-Modification systems against conjugation

Author(s): Tatiana Dimitriu, Mark D. Szczelkun, Edze R. Westra
Published in: bioRXiv, 2024
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.06.17.599295

Datasets

Datasets via OpenAIRE (2)

Source data for Naqvi et al Nature Chemical Biology 2022

Author(s): Szczelkun, Mark
Published in: University of Bristol

ENDO-Pore test files

Author(s): Szczelkun, Mark; Montaguth, Oscar Enrique Torres
Published in: University of Bristol