Periodic Reporting for period 3 - CODE (Coincidence detection of proteins and lipids in regulation of cellular membrane dynamics)
Période du rapport: 2022-01-01 au 2023-06-30
and protein localisation, and we will investigate coincidence detection of the membrane lipid phosphatidylinositol 3‐phosphate (PI3P) and various proteins as a paradigm for how coincidence detection controls protein localisation, membrane contact site formation, and membrane dynamics. We will screen
for new coincidence detectors using bioinformatics, quantitative proteomics and molecular biology approaches. Candidates from the screen will be verified experimentally as genuine coincidence detectors, and their functions in cellular membrane dynamics will be identified using genome editing and newly developed imaging methods. Artificial coincidence detectors with novel specificities will be created by genetic engineering, and these will be used for detection and manipulation of membrane dynamics processes. Engineered coincidence detectors will then be used as components of artificial cells with chemotactic properties. The success of this project will not only establish a theoretical framework for a fundamental principle in cell biology but also provide new potential pharmacological targets and novel tools in cell biology and biotechnology.