Novel microengineered environments for mouse embryonic stem cell (mESC) differentiation towards cardiomyocytes

Project Information

NEVADA

Grant agreement ID: 792776

DOI 10.3030/792776

Project closed

EC signature date 26 April 2018

Start date 1 September 2018

End date 1 October 2020

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 175 419,60

EU contribution € 175 419,60

Coordinated by

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

Objective

Cardiomyocytes (CMs) are the contractile cells of the heart. Their regenerative capacity is lost after birth. Embryonic stem cells (ESCs) are able to differentiate into functional CMs, however this process is not yet efficient. The elucidation of ESCs’ differentiation into CMs could provide an additional avenue for therapeutic interventions and disease modeling. This interdisciplinary project will result in a unique platform that targets precise quantification of the fundamental molecular mechanisms underlying mouse ESC cardiac differentiation at the single cell level. The platform takes the advantages of 3D tailored conductive microscaffolds (3DTCMSs) to deliver precise mechanical and electrical stimulation patterns to individual cells in vitro. Co-integrated 3DTCMSs have the necessary infrastructure for long-term cell culturing. The platform is accessible for in situ high-resolution, real-time microscopic observation of essential molecular information due to transparent materials. Advanced two-photon polymerization photolithography will be utilized for the exact reconstruction of 3DTCMSs based on a novel optically transparent, conductive ionic liquid-polymer composite. ESC pluripotent factors and CM markers will be monitored comparatively by live microscopy and molecular analysis. This project aims to integrate expertise in materials science, engineering, cellular and molecular biology. As a result, a novel in vitro CM differentiation model will be developed, which has a potential to open a completely new window of research in systems biology.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• engineering and technology › materials engineering › composites
• natural sciences › physical sciences › optics › microscopy
• medical and health sciences › medical biotechnology › cells technologies › stem cells
• natural sciences › biological sciences › molecular biology

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Programme(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Topic(s)

• MSCA-IF-2017 - Individual Fellowships

Call for proposal

H2020-MSCA-IF-2017

Funding Scheme

MSCA-IF-EF-ST - Standard EF

Coordinator

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH

• Net EU contribution: € 175 419,60
• Address: Raemistrasse 101 ; 8092 Zuerich ; Switzerland
• Region: Schweiz/Suisse/Svizzera › Zürich › Zürich
• Activity type: Higher or Secondary Education Establishments
• Total cost: € 175 419,60