Towards a New Frontier in Autophagy Inhibition: The Development of a Novel Class of Chemical Probes and Identification of Their Associated Biological Target

Información del proyecto

inhibitAUTO

Identificador del acuerdo de subvención: 794259

DOI

10.3030/794259

Proyecto finalizado el 31 Agosto 2018

Fecha de la firma de la CE 19 Febrero 2018

Fecha de inicio 1 Marzo 2018

Fecha de finalización 29 Febrero 2020

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 171 460,80

Aportación de la UE

€ 171 460,80

Coordinado por

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany

Periodic Reporting for period 1 - inhibitAUTO (Towards a New Frontier in Autophagy Inhibition: The Development of a Novel Class of ChemicalProbes and Identification of Their Associated Biological Target)

Período documentado: 2018-03-01 hasta 2020-02-29

Resumen del contexto y de los objetivos generales del proyecto

The dysregulation of the lysosomal degradation (autophagy) pathway has been implicated in several degenerative conditions including cancer. The development of efficient measures to control autophagy therefore potentially offers a desperately needed pathway through which cancer progression may be regulated. Around a quarter of deaths in Europe are currently attributed to cancer, and it is estimated that 90% of these deaths are caused by metastases. Inhibition of autophagy has recently been shown to block cell migration and prevent metastases in tumour models.

This action aims to discover a novel class of autophagy inhibitors and newly develop chemical probes that can identify their protein targets, and dissect their biological mode of action in relation to autophagy modulation.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

An enabling synthetic method was developed and applied to prepare a collection of compounds that fused an sp3-rich ring system with an sp2-rich ring system (~50 compounds). These compounds are structurally related to a series of autophagy inhibitors (“oxautins”) that were previously identified by the Waldmann group. The biological activity of the compounds was investigated in a range of cell-based phenotypic assays (including assays that monitor glucose uptake, hedgehog signalling, and autophagy pathways) and they were found to inhibit autophagy with sub-micromolar activity. To investigate the structure activity relationship, a further 15 analogues of the lead compound were prepared, enabling the identification of a new lead compound with nanomolar potency.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

A new class of autophagy inhibitors with considerably higher potency than the originally developed compound series (“oxautins”) has been realised (nM activity for the new lead compound, where only uM activity was exhibited by the previous lead compound series, oxautins). Studies will now investigate the minimum pharmacophore required for activity, as well as probe the mode of action through chemical biology techniques, in order to try to identify the molecular target(s) associated with the inhibitory activity observed. It is anticipated that the findings of this project will broadly have value towards increasing the understanding of unknown biological mechanisms underlying degenerative diseases such as cancer, which, ultimately, may aid the development of effective new treatments in future.

Fluorescence microscopy images for the cell based autophagy assay.