Project description
Nanoparticle-based sensors of miRNAs
Biosensors are analytical devices that employ biological components such as enzymes, antibodies, or nucleic acids to detect and measure specific substances in samples. The biological component interacts with the target analyte to produce a measurable electrical, optical, or electrochemical signal. Biosensors have attracted great interest for diagnostic and therapeutic applications. Funded by the Marie Skłodowska-Curie Actions programme, the Upbiosens project will develop a novel nanoparticle-based biosensor that incorporates DNA strands to detect miRNAs, small RNA molecules that serve as regulators of gene expression. Researchers will use fluorophores for quantitative measurement of nucleic acids, extending the applicability of these novel nanosensors to gene therapy and forensic analysis.
Objective
The Upbiosens project describes a novel method to produce water-dispersible and stable DNA-capped upconversion nanoparticles (UCNPs), keeping intact (or enhancing) the properties of both DNA and UCNPs. The UCNP@DNA nanohybrid will be able to detect the complementary target miRNA or DNA sequence. This detection will be based on the UCNP emission changes after NIR excitation in the presence of the fluorophore target RNA/DNA sequence. The hybridization will enable FRET from the UCNP to the fluorophore upon NIR irradiation, which will allow the quantitative measurement of nucleic acid. In addition, this new strategy for DNA functionalization of UCNPs will enable the control of the UCNP size during the functionalization step as well as the distance between UCNP and the energy acceptor. Both issues are critical for the design of novel, rapid, highly selective and sensitive FRET-based biosensors. This approach will be demonstrated for wo different compositions of UCNPs, NaYF4: Yb, Er (excitation at 980 nm) and NaYF4: Yb, Nd, Er (excitation at 800 nm) in order to enhance optical properties. The excitation at 800 nm reduces the overheating of biosamples due to the light absorption by water.
Taking into account the exceptional UCNPs emissive properties, the capability for in vitro nucleic acid imaging of the nanobiosensor will also be evaluated. To our knowledge, the combination of a nucleic acid sensing and imaging using UCNP-based nanohybrids has not yet been attempted. This dual NIR NA biosensor/bioimaging nanohybrid can be promisingly bio-implemented, such as in prognosis, diagnostics and treatment of diseases, gene therapy, or forensic analysis.
Fields of science
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesgeneticsRNA
- engineering and technologynanotechnologynano-materials
Programme(s)
Funding Scheme
MSCA-IF-EF-ST - Standard EFCoordinator
75794 Paris
France