Description du projet
Capteurs de miARN à base de nanoparticules
Les biocapteurs sont des dispositifs analytiques qui utilisent des composants biologiques tels que des enzymes, des anticorps ou des acides nucléiques pour détecter et mesurer des substances spécifiques dans des échantillons. Le composant biologique interagit avec l’analyte cible pour produire un signal électrique, optique ou électrochimique mesurable. Les biocapteurs ont suscité un grand intérêt pour les applications diagnostiques et thérapeutiques. Financé par le programme Actions Marie Skłodowska-Curie, le projet Upbiosens développera un nouveau biocapteur à base de nanoparticules qui incorpore des brins d’ADN pour détecter les miARN, de petites molécules d’ARN qui servent de régulateurs de l’expression des gènes. Les chercheurs utiliseront des fluorophores pour mesurer quantitativement les acides nucléiques, ce qui permettra d’étendre l’application de ces nouveaux nanocapteurs à la thérapie génique et à l’analyse médico-légale.
Objectif
The Upbiosens project describes a novel method to produce water-dispersible and stable DNA-capped upconversion nanoparticles (UCNPs), keeping intact (or enhancing) the properties of both DNA and UCNPs. The UCNP@DNA nanohybrid will be able to detect the complementary target miRNA or DNA sequence. This detection will be based on the UCNP emission changes after NIR excitation in the presence of the fluorophore target RNA/DNA sequence. The hybridization will enable FRET from the UCNP to the fluorophore upon NIR irradiation, which will allow the quantitative measurement of nucleic acid. In addition, this new strategy for DNA functionalization of UCNPs will enable the control of the UCNP size during the functionalization step as well as the distance between UCNP and the energy acceptor. Both issues are critical for the design of novel, rapid, highly selective and sensitive FRET-based biosensors. This approach will be demonstrated for wo different compositions of UCNPs, NaYF4: Yb, Er (excitation at 980 nm) and NaYF4: Yb, Nd, Er (excitation at 800 nm) in order to enhance optical properties. The excitation at 800 nm reduces the overheating of biosamples due to the light absorption by water.
Taking into account the exceptional UCNPs emissive properties, the capability for in vitro nucleic acid imaging of the nanobiosensor will also be evaluated. To our knowledge, the combination of a nucleic acid sensing and imaging using UCNP-based nanohybrids has not yet been attempted. This dual NIR NA biosensor/bioimaging nanohybrid can be promisingly bio-implemented, such as in prognosis, diagnostics and treatment of diseases, gene therapy, or forensic analysis.
Champ scientifique
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesgeneticsRNA
- engineering and technologynanotechnologynano-materials
Programme(s)
Régime de financement
MSCA-IF-EF-ST - Standard EFCoordinateur
75794 Paris
France