Objectif This proposal concerns Reversible Self-assembled Monolayers (rSAMs) as dynamic nanoparticle shells for multivalent interactions at biointerfaces. Current drug design and diagnostics are exploring the multivalency concept, i.e. binding of biological targets via multiple weak interactions. In contrast to classical drug design relying on high-affinity inhibitors, thisrelies on dendritic architectures featuring a high density of ligands, e.g. saccharides, capable of simultaneously interacting with biointerfacial receptors. This strategy can be used to inhibit the virus entry by blocking the receptor at the early stages of infection and the concept is being explored as antiviral drugs and in virus sensing. However, in current systems ligands are covalently fixed on the particle surface. This prevents control over the ligand distribution and composition which compromises selectivity and affinity of the interactions. rSAMs are pH-switchable versions of thiol-SAMs. They are tunable with respect to the nature of the head group and layer order and stability while featuring pH responsiveness and the dynamic nature of non-covalently build assemblies e.g. lipid bilayers. Ligand decorated rSAMs therefore feature strongly enhanced affinities for multivalent targets. The main aims of this proposal are:1) to investigate the use of rSAMs as dynamic nanoparticle shells for multivalent inhibition of viruses and 2) to assess suchsystems as nanoplasmonic sensors for antibody-free ultrasensitive, robust and rapid in situ virus detection.Under 1) we will select model pathogens, e.g. Ebola and prepare a series of saccharide terminated amidines for the first generation dynamic shell nanoparticles.Their efficiency will be assessed in infection assays using artificial virus particlesUnder 2) we will develop influenza virus sensors with subtyping capability within human and animal virus strains. The sensors will be validated with respect to benchmark assays. Champ scientifique natural sciencesbiological sciencesmicrobiologyvirologymedical and health sciencesbasic medicinemedicinal chemistrymedical and health scienceshealth sciencesinfectious diseasesRNA virusesinfluenzaengineering and technologynanotechnologynano-materialsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2017 - Individual Fellowships Appel à propositions H2020-MSCA-IF-2017 Voir d’autres projets de cet appel Régime de financement MSCA-IF-EF-CAR - CAR – Career Restart panel Coordinateur MALMO UNIVERSITET Contribution nette de l'UE € 185 857,20 Adresse NORDENSKIOLDSGATAN 1 205 06 Malmoe Suède Voir sur la carte Région Södra Sverige Sydsverige Skåne län Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 185 857,20
