Periodic Reporting for period 1 - EpiTFeb (Deciphering the Transcriptional Logic of TFEB activation in cell identity and cancer)
Reporting period: 2018-04-01 to 2020-03-31
Transcriptional regulation is a crucial process not only to instruct developmental transitions but also to coordinate organismal cellular processes under homeostasis and stress. The proposed project aims at providing a systematic dissection of the molecular and developmental cues responsible for TFEB transcriptional regulation, an aspect that is still missing.
Overall, the project performed so far provided:
- a deep characterization of the transcriptional repertoire of different cell types under specific stimuli (i.e. starvation and induction of differentiation);
- the identification of bonafide TFEB transcriptional regulators;
- functional validation of candidate transcription factors.
Indeed, I identified transcription factors that drive TFEB expression and characterized the mode of actions of these factors. Moreover, by using genomic approaches, I was able to establish the relationship between the genomic sequences and the regulatory activity of TFEB cis-regulatory elements.