Objective This project addresses the quest for a new nanoparticle-based biosensing platform to study the chemical signaling within bacterial microbiome, bridging the gap from nanotechnology to biodiagnostic application. Healing-impaired and chronic wounds are a snowballing threat to public health and the economy. Their efficient treatment requires insight into the local wound microbiome, namely the composition and temporal evolution of bacterial communities. This project aims to provide access to the chemical cell-to-cell communication (quorum sensing, QS), which will enable monitoring changes in biofilms, specifically the competition of bacterial communities for resources and space, and the population density-depended change from non-virulent to virulent. To realize this goal, NANOBIOME will develop a plasmonic 2D screening platform for ultrasensitive surface-enhanced Raman scattering (SERS) detection of bacterial QS signaling. Contrary to other approaches, NANOBIOME will build on (1) well-defined self-assembled plasmonic nanoclusters, acting as sensing pixels; and (2) their ordered assembly on solid supports with defined inter-particle spacing - to avoid plasmonic coupling between pixels. Further modifications of the nanostructures will allow for facile tailoring of optical properties ideally suited for rapid screening for highest SERS activity. This SERS screening platform will grant quantitative insight into intra- and interspecies signaling to improve our understanding of bacterial competition in wound microbiomes using bacterial model systems (e.g. pathogens versus commensal bacteria). Fields of science medical and health scienceshealth sciencespublic healthengineering and technologyenvironmental biotechnologybiosensingnatural sciencesbiological sciencesmicrobiologybacteriologyengineering and technologynanotechnology Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2017 - Individual Fellowships Call for proposal H2020-MSCA-IF-2017 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE Net EU contribution € 158 121,60 Address PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 20009 San Sebastian Spain See on map Region Noreste País Vasco Gipuzkoa Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 158 121,60
