Objectif Eutypiose is a grapevine die back disease which has spread through out the European vineyard over the last decade. The causal agent is Eutypa lata (ascomycetum) and seriousness of the disease has been under-estimated since a long incubation period preceed the destruction of the plant (3 to 8 years) and symptoms expression during vegetation, the only available assessment of plant health is dependant on climatic conditions of the bud-burst period. At the moment no curative method has been developed and only prophylactic and preventive control exist. The project will have as main objectives to (1) develop methods of prophylactic and preventive control to limit spread of the disease; (2) create tool(s) for early detection; (3) improve or create new methods for controling disease including curative one. Eutypiose is a fungal disease causing die back of grapevine which has spread throughout European vineyards over the last decade. The causal agent is Eutypa lata (ascomycetum) and the only expression of symptons occurs during the bud burst period but these are revealed only if climatic conditions are favourable. No cure exists at present, although preventive control and prophylactic measures are available. Research is underway to: develop methods of prophylactic and preventive control to limit spread of the disease; create tools for early detection; and improve or create new methods for controlling the disease including curative ones.An observation network to assess the evolution, scale and importance of the disease has been set up covering wine growing areas in France, Spain, Portugal, Italy and Greece. First results suggest that expression of symptons following bud burst is generally less important in other countries than in France. Experiments conducted on control methods such as cutting down vine stocks and fungicide sprays have demonstrated low efficacy. Research on early detection has been initiated by grafting fungal eutypine toxin on a carrier protein. Antibodies have been obtained but their titres were low. In work on the host parasite relationship, Eutypa lata strains have been selected, one aggressive and the other not. The kinetics of degradation of wood has been studied and related enzymes determined. First results on receptivity to fungal spores, following wood pruning, suggest no difference between vine stocks and that later pruning reduces infectivity. Specific studies have commenced with eutypin toxin and cytological studies have indicated an alteration of chloropasts in diseased plants.The experiments will include : (1) dissemination of technology and setting up an observation network to assess the scale of the disease (importance and evolution); (2) agronomic experiments to study the modes of expression of symptoms during vegetation (under different climatic conditions) and to define new control; (3) development and dissemination of existing control methods among wine growers; (4) development of an early diagnostic test; (5) and (6) studies on host-parasite relationship (attack and propagation of the fungus in wood) and on Eutypine (toxin synthetized by the fungus). Champ scientifique agricultural sciencesagriculture, forestry, and fisheriesagriculturehorticultureviticultureagricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant protectionnatural sciencesbiological sciencesmicrobiologymycologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesagricultural sciencesagriculture, forestry, and fisheriesforestry Programme(s) FP2-CAMAR - Research and technological development programme (EEC) in the field of competitiveness of agriculture and management of agricultural resources, 1989-1993 Thème(s) Data not available Appel à propositions Data not available Régime de financement Data not available Coordinateur MOET HENNESSY LOUIS VUITTON Contribution de l’UE Aucune donnée Adresse 30 AVENUE HOCHE 75008 PARIS France Voir sur la carte Coût total Aucune donnée Participants (8) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire INSTITUT NATIONAL POLYTECHNIQUE DE TOULOUSE France Contribution de l’UE Aucune donnée Adresse Avenue de Muret 145 31076 TOULOUSE Voir sur la carte Coût total Aucune donnée INSTITUTO NACIONAL DE INVESTIGACAO AGRARIA - CENTRO NACIONAL DE PROTECCAO DA PRODUCAO AGRICOLA Portugal Contribution de l’UE Aucune donnée Adresse QUINTA DO MARQUES 2780 OEIRAS Voir sur la carte Coût total Aucune donnée Institut National de la Recherche Agronomique (INRA) France Contribution de l’UE Aucune donnée Adresse 147 rue de l'Université 75341 Paris Voir sur la carte Coût total Aucune donnée MINISTERE DE L'AGRICULTURE France Contribution de l’UE Aucune donnée Adresse 175 RUE DU CHEVALERET 75646 PARIS Voir sur la carte Coût total Aucune donnée MINISTRY OF AGRICULTURE - PLANT PROTECTION INSTITUTE Grèce Contribution de l’UE Aucune donnée Adresse PHYTOKO P.O.B. 303 38001 VOLOS Voir sur la carte Coût total Aucune donnée UNIVERSIDAD POLITECNICA DE MADRID - LABORATORIO DE PATOLOGIA VEGETAL Espagne Contribution de l’UE Aucune donnée Adresse CIUDAD UNIVERSITARIA 28040 MADRID Voir sur la carte Coût total Aucune donnée UNIVERSITE DE BORDEAUX I France Contribution de l’UE Aucune donnée Adresse AVENUE LEON DUGUIT 33604 PESSAC Voir sur la carte Coût total Aucune donnée Università degli Studi di Milano Italie Contribution de l’UE Aucune donnée Adresse Celoria 2 20133 Milano Voir sur la carte Coût total Aucune donnée