Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy

Deliverables

Applications, grant proposals and theses on Biocatalysis

Applications, grant proposals and theses on spectroscopically guided Biocatalysis-based solutions for societal challenges, which will take place from month 6 through 45.

Events in form of mini workshops for non specialists

Organization of mini workshops at ITQB for researchers of other areas (like medicine, pharmaceutical and biotechnological companies) on i) Spectroscopy for illuminating metal trafficking and homeostasis in environmentii) Perspectives of spectroscopy in medical diagnosisiii) Interaction of light and matter, what can we see by shining light on object, cells and molecules? Months 13-43

Applications, grant proposals and theses on Metals in Biology

Applications, grant proposals and theses on understanding of biological metal homeostasis and trafficking, which will occur from month 6 to 45.

Communications in conferences and scientific journals

Scientific Communications in conferences and scientific journals, throughout the project duration.

Events for scientific training of students and researchers

Aprox. 11 students/researchers trained in three Workshops on: Enzyme engineering and rational design of biocatalysts (month 44), Spectroscopy of proteins: biochemistry and vibrational spectroscopy on [NiFe]-hydrogenase (month 35) and NMR of paramagnetic proteins and applications (month 41).In addition, aprox. 80 students/researchers trained in three Training schools on: Metals in biology (month 6), Advanced vibrational biospectroscopy in life sciences (month 31) and Fundamentals of magnetic resonance spectroscopy and applications (month 40).

Events for training of researchers in soft skills

Training of researchers (~40) in scientific writing, science communication, grant writing and IP Protection, through two Winter schools on Scientific writing and Science communication (months 12 and 24, respectively)

Patent Application

Patent application based on exploitable results identified after the conclusion of the exchange activities performed in WP3 and WP4

Web-based Virtual Platform

Organization of Webbased Virtual Platform for biospectroscopy

Production of communication materials

Establish an efficient dissemination, exploitation and communication plan and monitor its implementation, together with production of communication materials (on-line communication mainly and posters, flyers, booklets, short videos, electronic newsletter or other), during months 5-6.

Publications

Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR

Author(s): Luchinat E, Barbieri L, Cremonini M, Nocentini A, Supuran CT, Banci L
Published in: ACS Chemical Biology, 2020, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.0c00590

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

Author(s): Maglio O, Chino M, Vicari C, Pavone V, Louro RO, Lombardi A.
Published in: Chemical Communications, 2021, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc06676a

Electroactivity across the cell wall of Gram-positive bacteria

Author(s): Paquete CM
Published in: Computational and Structural Biotechnology Journal, 2020, ISSN 2001-0370
Publisher: Elsevier
DOI: 10.1016/j.csbj.2020.11.021

Proteomic and Isotopic Response of Desulfovibrio vulgaris to DsrC Perturbation

Author(s): Leavitt WD, Venceslau SS, Waldbauer J, Smith DA, Pereira IAC, Bradley AS
Published in: Frontiers in Microbiology, 2019, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2019.00658

Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli.

Author(s): Trindade IB, Hernandez G, Lebègue E, Barrière F, Cordeiro T, Piccioli M, Louro RO
Published in: J Biol Inorg Chem, 2021, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-021-01854-y

“Out of Pocket” Protein Binding—A Dilemma of Epitope Imprinted Polymers Revealed for Human Hemoglobin

Author(s): Zhang X, Caserta G, Yarman A, Supala E, Waffo AFT, Wollenberger U, Gyurcsányi RE, Zebger I, Scheller FW
Published in: Chemosensors, 2021, ISSN 2227-9040
Publisher: MDPI AG
DOI: 10.3390/chemosensors9060128

High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli

Author(s): Fan Q, Caserta G, Lorent C, Zebger I, Neubauer P, Lenz O, Gimpel M.
Published in: Frontiers in Microbiology, 2022, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2022.894375

Hydroxy-bridged resting states of a [NiFe]-hydrogenase unraveled by cryogenic vibrational spectroscopy and DFT computations

Author(s): Caserta G, Pelmenschikov V, Lorent C, Tadjoung Waffo AF, Katz S, Lauterbach L, Schoknecht J, Wang H, Yoda Y, Tamasaku K, Kaupp M, Hildebrandt P, Lenz O, Cramer SP, Zebger I.
Published in: Chemical Science, 2020, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc05022a

A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples

Author(s): Amaral C, Antunes W, Moe E, Duarte AG, Lima LMP, Santos C, Gomes IL, Afonso GS, Vieira R, Teles HSS, Reis MS, da Silva MAR, Henriques AM, Fevereiro M, Ventura MR, Serrano M, Pimentel C.
Published in: Scientific Reports, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-95799-6

Crossing the Wall: Characterization of the Multiheme Cytochromes Involved in the Extracellular Electron Transfer Pathway of Thermincola ferriacetica.

Author(s): Faustino MM, Fonseca BM, Costa NL, Lousa D, Louro RO, Paquete CM
Published in: Microorganisms, 2021, ISSN 2076-2607
Publisher: Basel MDPI AG 2013
DOI: 10.3390/microorganisms9020293

Resonance Raman spectroscopic analysis of the iron–sulfur cluster redox chain of the Ralstonia eutropha membrane-bound [NiFe]-hydrogenase

Author(s): Siebert, E., Schmidt, A., Frielingsdorf, S., Kalms, J., Kuhlmann, U., Lenz, O., Scheerer, P., Zebger, I., Hildebrandt, P.
Published in: Journal of Raman Spectroscopy, 2021, ISSN 0377-0486
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jrs.6163

The large subunit of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha - a minimal hydrogenase?

Author(s): Caserta G, Lorent C, Ciaccafava A, Keck M, Breglia R, Greco C, Limberg C, Hildebrandt P, Cramer SP, Zebger I, Lenz O.
Published in: Chemical Science, 2020, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc01369b

Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives.

Author(s): Lopes RR, Tomé CS, Russo R, Paterna R, Leandro J, Candeias NR, Gonçalves LMD, Teixeira M, Sousa PMF, Guedes RC, Vicente JB, Gois PMP, Leandro P.
Published in: Biomolecules, 2021, ISSN 2218-273X
Publisher: MDPI AG
DOI: 10.3390/biom11030462

Redox-Polymer-Wired [NiFeSe] Hydrogenase Variants with Enhanced O2 Stability for Triple-Protected High-Current-Density H2 -Oxidation Bioanodes

Author(s): Ruff A, Szczesny J, Vega M, Zacarias S, Matias PM, Gounel S, Mano N, Pereira IAC, Schuhmann W.
Published in: ChemSusChem, 2020, ISSN 1864-564X
Publisher: ChemSusChem
DOI: 10.1002/cssc.202000999

Repair of Iron Center Proteins-A Different Class of Hemerythrin-like Proteins

Author(s): Silva LSO, Matias PM, Romão CV, Saraiva LM.
Published in: Molecules, Issue 14203049, 2022, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27134051

Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases.

Author(s): Silveira CM, Moe E, Fraaije M, Martins LO, Todorovic S.
Published in: RSC advances, 2020, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ra00950d

Molecular Details on Multiple Cofactor Containing Redox Metalloproteins Revealed by Infrared and Resonance Raman Spectroscopies

Author(s): Silveira CM, Zuccarello L, Barbosa C, Caserta G, Zebger I, Hildebrandt P, Todorovic S
Published in: Molecules, 2021, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26164852

How the anaerobic enteropathogen clostridioides difficile tolerates low O2 tensions

Author(s): Kint N, Alves Feliciano C, Martins MC, Morvan C, Fernandes SF, Folgosa F, Dupuy B, Texeira M, Martin-Verstraete I
Published in: mBio, 2020, ISSN 2161-2129
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.01559-20

PRE-driven protein NMR structures: an alternative approach in highly paramagnetic systems

Author(s): Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M.
Published in: FEBS Journal, 2021, ISSN 1742-4658
Publisher: Federation of European Biochemical Societies journal
DOI: 10.1111/febs.15615

An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity

Author(s): Amaral C, Vicente CT, Caetano SM, Gaspar-Cordeiro A, Yang Y, Cloetens P, Romão CV, Rodrigues-Pousada C, Pimentel C
Published in: Microorganisms, Issue 20762607, 2021, ISSN 2076-2607
Publisher: Basel MDPI AG 2013
DOI: 10.3390/microorganisms9061337

SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices

Author(s): Zuccarello L, Barbosa C, Galdino E, Lončar N, Silveira CM, Fraaije MW, Todorovic S
Published in: Int J Mol Sci., Issue 14220067, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22157998

Sequence-specific Assignments in NMR Spectra of paramagnetic systems: A non-systematic approach,

Author(s): Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M.
Published in: Inorg. Chim. Acta, Issue 00201693, 2021, ISSN 0020-1693
Publisher: Elsevier BV
DOI: 10.1016/j.ica.2020.119984

DsrL mediates electron transfer between NADH and rDsrAB in Allochromatium vinosum.

Author(s): Löffler M, Feldhues J, Venceslau SS, Kammler L, Grein F, Pereira IAC, Dahl C
Published in: Environmental Microbiology, 2020, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14899

Measuring transverse relaxation in highly paramagnetic systems

Author(s): Invernici M, Trindade IB, Cantini F, Louro RO, Piccioli M.
Published in: J Biomol NMR, Issue 09252738, 2020, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-020-00334-w

Integration of a Hydrogenase in a Lead Halide Perovskite Photoelectrode for Tandem Solar Water Splitting

Author(s): Edwardes Moore E, Andrei V, Zacarias S, Pereira IAC, Reisner E
Published in: ACS Energy Letters, Issue 23808195, 2019, ISSN 2380-8195
Publisher: American Chemical Society
DOI: 10.1021/acsenergylett.9b02437

Responses of Clostridia to oxygen: from detoxification to adaptive strategies

Author(s): Morvan C, Folgosa F, Kint N, Teixeira M, Martin-Verstraete I.
Published in: Environmental Microbiology, Issue 14622912, 2021, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15665

Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System

Author(s): Silva AV, Firmino MO, Costa NL, Louro RO, Paquete CM
Published in: Biomolecules, 2022, ISSN 2218-273X
Publisher: MDPI AG
DOI: 10.3390/biom12040549

Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis.

Author(s): Silva LSO, Matias PM, Romão CV, Saraiva LM.
Published in: Frontiers in Microbiology, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.670681

In Vitro Assembly as a Tool to Investigate Catalytic Intermediates of [NiFe]-Hydrogenase.

Author(s): Caserta G, Lorent C, Pelmenschikov V, Schoknecht J, Yoda Y, Hildebrandt P, Cramer SP, Zebger I, Lenz O
Published in: ACS Catalysis, 2020, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.0c04079

Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1

Author(s): Silva AV, Edel M, Gescher J, Paquete CM
Published in: Frontiers in Microbiology, 2020, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2020.00815

Sulfide and Carbon Monoxide Tolerance in Bacteria

Author(s): Silva LSO, Matias PM, Romão CV, Saraiva LM
Published in: Antioxidants, 2021, ISSN 2076-3921
Publisher: MDPI AG
DOI: 10.3390/antiox10050729

Staphylococcus aureus flavohaemoglobin contributes to early stage biofilm development under nitrosative stress

Author(s): Carvalho SM, Freitas CS, Oliveira AS, Saraiva LM
Published in: FEMS Microbiology Letters, 2021, ISSN 0378-1097
Publisher: Blackwell
DOI: 10.1093/femsle/fnab131

Disentangling Unusual Catalytic Properties and the Role of the [4Fe-4S] Cluster of Three Endonuclease III from the Extremophile D. radiodurans

Author(s): Rollo, F.; Borges, P.T.; Silveira, C.M.; Rosa, M.T.G.; Todorovic, S.; Moe, E.
Published in: Molecules, 2022, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27134270

The Amino Acids Motif -32GSSYN36- in the Catalytic Domain of E. coli Flavorubredoxin NO Reductase Is Essential for Its Activity

Author(s): Martins, M.C.; Fernandes, S.F.; Salgueiro, B.A.; Soares, J.C.; Romão, C.V.; Soares, C.M.; Lousa, D.; Folgosa, F.; Teixeira, M
Published in: Catalysts, Issue 20734344, 2021, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal11080926

Role of multiheme cytochromes involved in extracellular anaerobic respiration in bacteria

Author(s): Edwards MJ, Richardson DJ, Paquete CM, Clarke TA
Published in: Protein Science, 2020, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3787

Fast CO2 hydration kinetics impair heterogeneous but improve enzymatic CO2reduction catalysis

Author(s): Cobb SJ, Badiani VM, Dharani AM, Wagner A, Zacarias S, Oliveira AR, Pereira IAC, Reisner E.
Published in: Nature Chemistry, Issue 17554330, 2022, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-021-00880-2

The Di-iron RIC Protein (YtfE) of Escherichia coli Interacts with the DNA-Binding Protein from Starved Cells (Dps) To Diminish RIC Protein-Mediated Redox Stress

Author(s): Liliana S. O. Silva, Joana M. Baptista, Charlotte Batley, Simon C. Andrews, Lígia M. Saraiva
Published in: Journal of Bacteriology, Issue 200/24, 2018, ISSN 0021-9193
Publisher: American Society for Microbiology
DOI: 10.1128/jb.00527-18

An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation

Author(s): Américo G. Duarte, Teresa Catarino, Gaye F. White, Diana Lousa, Sinje Neukirchen, Cláudio M. Soares, Filipa L. Sousa, Thomas A. Clarke, Inês A. C. Pereira
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-07839-x

Identification of the sirohaem biosynthesis pathway in Staphylococcus aureus

Author(s): Marco A. M. Videira, Susana A. L. Lobo, Filipa L. Sousa, Lígia M. Saraiva
Published in: The FEBS Journal, 2019, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15091

The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

Author(s): Sandra P. Santos, Yang Yang, Margarida T. G. Rosa, Mafalda A. A. Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A. Carrondo, Peter Cloetens, Isabel A. Abreu, Célia V. Romão
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-53140-2

Metabolomics of Escherichia coli Treated with the Antimicrobial Carbon Monoxide-Releasing Molecule CORM-3 Reveals Tricarboxylic Acid Cycle as Major Target

Author(s): Sandra M. Carvalho, Joana Marques, Carlos C. Romão, Lígia M. Saraiva
Published in: Antimicrobial Agents and Chemotherapy, Issue 63/10, 2019, ISSN 0066-4804
Publisher: American Society for Microbiology
DOI: 10.1128/aac.00643-19

Interfacing Formate Dehydrogenase with Metal Oxides for the Reversible Electrocatalysis and Solar-Driven Reduction of Carbon Dioxide

Author(s): Melanie Miller, William E. Robinson, Ana Rita Oliveira, Nina Heidary, Nikolay Kornienko, Julien Warnan, Inês A. C. Pereira, Erwin Reisner
Published in: Angewandte Chemie International Edition, Issue 58/14, 2019, Page(s) 4601-4605, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201814419

How Thermophilic Gram-Positive Organisms Perform Extracellular Electron Transfer: Characterization of the Cell Surface Terminal Reductase OcwA

Author(s): N. L. Costa, B. Hermann, V. Fourmond, M. M. Faustino, M. Teixeira, O. Einsle, C. M. Paquete, R. O. Louro
Published in: mBio, Issue 10/4, 2019, ISSN 2150-7511
Publisher: American Society for microbiology
DOI: 10.1128/mbio.01210-19

How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes

Author(s): Maria C. Martins, Célia V. Romão, Filipe Folgosa, Patrícia T. Borges, Carlos Frazão, Miguel Teixeira
Published in: Free Radical Biology and Medicine, Issue 140, 2019, Page(s) 36-60, ISSN 0891-5849
Publisher: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2019.01.051

Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition

Author(s): Veronica Palma Delgado, Catarina M. Paquete, Gunnar Sturm, Johannes Gescher
Published in: Bioelectrochemistry, Issue 129, 2019, Page(s) 18-25, ISSN 1567-5394
Publisher: Elsevier BV
DOI: 10.1016/j.bioelechem.2019.04.022

Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility

Author(s): Inês B. Trindade, José M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro
Published in: Journal of Biological Chemistry, Issue 294/1, 2019, Page(s) 157-167, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.005041

Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic

Author(s): Katarzyna P. Sokol, William E. Robinson, Ana R. Oliveira, Sonia Zacarias, Chong-Yong Lee, Christopher Madden, Arnau Bassegoda, Judy Hirst, Inês A. C. Pereira, Erwin Reisner
Published in: Journal of the American Chemical Society, Issue 141/44, 2019, Page(s) 17498-17502, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b09575

A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase

Author(s): Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira
Published in: ACS Catalysis, Issue 9/9, 2019, Page(s) 8509-8519, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.9b02347

Photoreduction of CO 2 with a Formate Dehydrogenase Driven by Photosystem II Using a Semi-artificial Z-Scheme Architecture

Author(s): Katarzyna P. Sokol, William E. Robinson, Ana R. Oliveira, Julien Warnan, Marc M. Nowaczyk, Adrian Ruff, Inês A. C. Pereira, Erwin Reisner
Published in: Journal of the American Chemical Society, Issue 140/48, 2018, Page(s) 16418-16422, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b10247

Optimizing Electroactive Organisms: The Effect of Orthologous Proteins

Author(s): Bruno M. Fonseca, Luís Silva, Inês B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete
Published in: Frontiers in Energy Research, Issue 7, 2019, ISSN 2296-598X
Publisher: Frontiers Media S.A.
DOI: 10.3389/fenrg.2019.00002

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells

Author(s): Karim Zuhra, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, Alessandro Giuffrè
Published in: Cells, Issue 8/8, 2019, Page(s) 828, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells8080828

Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode

Author(s): Estelle Lebègue, Nazua L. Costa, Bruno M. Fonseca, Ricardo O. Louro, Frédéric Barrière
Published in: Journal of Electroanalytical Chemistry, Issue 847, 2019, Page(s) 113232, ISSN 1572-6657
Publisher: Elsevier BV
DOI: 10.1016/j.jelechem.2019.113232

Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery

Author(s): Veronica Nasta, Stefano Da Vela, Spyridon Gourdoupis, Simone Ciofi-Baffoni, Dmitri I. Svergun, Lucia Banci
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-55313-5

Methylglyoxal interaction with superoxide dismutase 1

Author(s): Panagis Polykretis, Enrico Luchinat, Francesca Boscaro, Lucia Banci
Published in: Redox Biology, Issue 30, 2020, Page(s) 101421, ISSN 2213-2317
Publisher: Elsevier BV
DOI: 10.1016/j.redox.2019.101421

X-ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases

Author(s): Yulia Ilina, Christian Lorent, Sagie Katz, Jae-Hun Jeoung, Seigo Shima, Marius Horch, Ingo Zebger, Holger Dobbek
Published in: Angewandte Chemie International Edition, Issue 58/51, 2019, Page(s) 18710-18714, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201908258

NMR of paramagnetic metalloproteins in solution: Ubi venire, quo vadis?

Author(s): Trindade IB, Coelho A, Cantini F, Piccioli M, Louro RO.
Published in: J Inorg Biochem, Issue 01620134, 2022, ISSN 0162-0134
Publisher: Elsevier BV
DOI: 10.1016/j.jinorgbio.2022.111871

A New Paradigm of Multiheme Cytochrome Evolution by Grafting and Pruning Protein Modules

Author(s): Soares R, Costa NL, Paquete CM, Andreini C, Louro RO
Published in: Mol Biol Evol, Issue 15371719, 2022, ISSN 1537-1719
Publisher: Oxford University Press
DOI: 10.1093/molbev/msac139

Synergetic Antimicrobial Activity and Mechanism of Clotrimazole- Linked CO-Releasing Molecules

Author(s): Sofia S. Mendes, Joana Marques, Edit Mesterházy, Jan Straetener, Melina Arts, Teresa Pissarro, Jorgina Reginold, Anne Berscheid, Jan Bornikoel, Robert M. Kluj, Christoph Mayer, Filipp Oesterhelt, Sofia Friães, Beatriz Royo, Tanja Schneider, Heike Brötz-Oesterhelt, Carlos C. Romão, and Lígia M. Saraiva
Published in: ACS Bio & Med Chem Au, Issue 26942437, 2022, ISSN 2694-2437
Publisher: American Chemical Society
DOI: 10.1021/acsbiomedchemau.2c00007

Let's chat: Communication between electroactive microorganisms

Author(s): Paquete CM, Rosenbaum MA, Bañeras L, Rotaru AE, Puig S.
Published in: Bioresource Technology, 2022, ISSN 0960-8524
Publisher: Elsevier BV
DOI: 10.1016/j.biortech.2022.126705

The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase

Author(s): Ferreira D, Barbosa ACC, Oliveira GP, Catarino T, Venceslau SS, Pereira IAC
Published in: PNAS, 2022, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2118880119

Structural and spectroscopic characterization of a HdrA-like subunit from Hyphomicrobium denitrificans

Author(s): Ernst C, Kayastha K, Koch T, Venceslau SS, Pereira IAC, Demmer U, Ermler U, Dahl C.
Published in: FEBS Journal, 2021, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15505

Unusual structures and unknown roles of Fes clusters in metalloenzymes seen from a resonance Raman Spectroscopic Perspective

Author(s): Caserta, G.; Zuccarello, L.; Barbosa, C.; Silveira, C. M.; Moe, E.; Katz, S.; Hildebrandt, P.; Zebger, I.; Todorovic, S.
Published in: Coordination Chemistry Reviews, 2022, ISSN 0010-8545
Publisher: Elsevier BV
DOI: 10.1016/j.ccr.2021.214287

In Vitro Assembly Exploring Structure and Function of Redox Intermediates in [NiFe]-Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes

Author(s): C. Lorent, V. Pelmenschikov, S. Frielingsdorf, J. Schoknecht, G. Caserta, Y. Yoda, H. Wang, K. Tamasaku, O. Lenz, S.P. Cramer, M. Horch, L. Lauterbach and I. Zebger
Published in: Angewandte Chemie Int, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202100451

Mutational and structural analysis of an ancestral fungal dye-decolorizing peroxidase

Author(s): Zitare UA, Habib MH, Rozeboom H, Mascotti ML, Todorovic S, Fraaije MW.
Published in: FEBS Journal, 2021, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15687

Unique Biradical Intermediate in the Mechanism of the Heme Enzyme Chlorite Dismutase

Author(s): Püschmann J, Mahor D, de Geus DC, Strampraad MJF, Srour B, Hagen WR, Todorovic S, Hagedoorn PL
Published in: ACS Catalysis, 2021, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c03432

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Author(s): Zuccarello, L.; Barbosa, C.; Todorovic, S.; Silveira, C.M.
Published in: Catalysts, 2021, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal11020218

Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli.

Author(s): Fan Q, Caserta G, Lorent C, Lenz O, Neubauer P, Gimpel M.
Published in: Microorganisms, 2021, ISSN 2076-2607
Publisher: MDPI
DOI: 10.3390/microorganisms9061195

Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing

Author(s): Barbosa C, Silveira CM, Silva D, Brissos V, Hildebrandt P, Martins LO, Todorovic S
Published in: Biosensors and bioelectronics, 2020, ISSN 0956-5663
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2020.112055

Copper Acts Synergistically With Fluconazole in Candida glabrata by Compromising Drug Efflux, Sterol Metabolism, and Zinc Homeostasis.

Author(s): Gaspar-Cordeiro A, Amaral C, Pobre V, Antunes W, Petronilho A, Paixão P, Matos AP, Pimentel C.
Published in: Frontiers in Microbiology, Issue 1664302X, 2022, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2022.920574

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