Twin to Illuminate Metals in Biology and Biocatalysis through Biospectroscopy

Livrables

Applications, grant proposals and theses on Biocatalysis

Applications, grant proposals and theses on spectroscopically guided Biocatalysis-based solutions for societal challenges, which will take place from month 6 through 45.

Events in form of mini workshops for non specialists

Organization of mini workshops at ITQB for researchers of other areas (like medicine, pharmaceutical and biotechnological companies) on i) Spectroscopy for illuminating metal trafficking and homeostasis in environmentii) Perspectives of spectroscopy in medical diagnosisiii) Interaction of light and matter, what can we see by shining light on object, cells and molecules? Months 13-43

Applications, grant proposals and theses on Metals in Biology

Applications, grant proposals and theses on understanding of biological metal homeostasis and trafficking, which will occur from month 6 to 45.

Communications in conferences and scientific journals

Scientific Communications in conferences and scientific journals, throughout the project duration.

Events for scientific training of students and researchers

Aprox. 11 students/researchers trained in three Workshops on: Enzyme engineering and rational design of biocatalysts (month 44), Spectroscopy of proteins: biochemistry and vibrational spectroscopy on [NiFe]-hydrogenase (month 35) and NMR of paramagnetic proteins and applications (month 41).In addition, aprox. 80 students/researchers trained in three Training schools on: Metals in biology (month 6), Advanced vibrational biospectroscopy in life sciences (month 31) and Fundamentals of magnetic resonance spectroscopy and applications (month 40).

Events for training of researchers in soft skills

Training of researchers (~40) in scientific writing, science communication, grant writing and IP Protection, through two Winter schools on Scientific writing and Science communication (months 12 and 24, respectively)

Patent Application

Patent application based on exploitable results identified after the conclusion of the exchange activities performed in WP3 and WP4

Web-based Virtual Platform

Organization of Webbased Virtual Platform for biospectroscopy

Production of communication materials

Establish an efficient dissemination, exploitation and communication plan and monitor its implementation, together with production of communication materials (on-line communication mainly and posters, flyers, booklets, short videos, electronic newsletter or other), during months 5-6.

Publications

Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR

Auteurs: Luchinat E, Barbieri L, Cremonini M, Nocentini A, Supuran CT, Banci L
Publié dans: ACS Chemical Biology, 2020, ISSN 1554-8929
Éditeur: American Chemical Society
DOI: 10.1021/acschembio.0c00590

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

Auteurs: Maglio O, Chino M, Vicari C, Pavone V, Louro RO, Lombardi A.
Publié dans: Chemical Communications, 2021, ISSN 1359-7345
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0cc06676a

Electroactivity across the cell wall of Gram-positive bacteria

Auteurs: Paquete CM
Publié dans: Computational and Structural Biotechnology Journal, 2020, ISSN 2001-0370
Éditeur: Elsevier
DOI: 10.1016/j.csbj.2020.11.021

Proteomic and Isotopic Response of Desulfovibrio vulgaris to DsrC Perturbation

Auteurs: Leavitt WD, Venceslau SS, Waldbauer J, Smith DA, Pereira IAC, Bradley AS
Publié dans: Frontiers in Microbiology, 2019, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2019.00658

Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli.

Auteurs: Trindade IB, Hernandez G, Lebègue E, Barrière F, Cordeiro T, Piccioli M, Louro RO
Publié dans: J Biol Inorg Chem, 2021, ISSN 0949-8257
Éditeur: Springer Verlag
DOI: 10.1007/s00775-021-01854-y

“Out of Pocket” Protein Binding—A Dilemma of Epitope Imprinted Polymers Revealed for Human Hemoglobin

Auteurs: Zhang X, Caserta G, Yarman A, Supala E, Waffo AFT, Wollenberger U, Gyurcsányi RE, Zebger I, Scheller FW
Publié dans: Chemosensors, 2021, ISSN 2227-9040
Éditeur: MDPI AG
DOI: 10.3390/chemosensors9060128

High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli

Auteurs: Fan Q, Caserta G, Lorent C, Zebger I, Neubauer P, Lenz O, Gimpel M.
Publié dans: Frontiers in Microbiology, 2022, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2022.894375

Hydroxy-bridged resting states of a [NiFe]-hydrogenase unraveled by cryogenic vibrational spectroscopy and DFT computations

Auteurs: Caserta G, Pelmenschikov V, Lorent C, Tadjoung Waffo AF, Katz S, Lauterbach L, Schoknecht J, Wang H, Yoda Y, Tamasaku K, Kaupp M, Hildebrandt P, Lenz O, Cramer SP, Zebger I.
Publié dans: Chemical Science, 2020, ISSN 2041-6520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0sc05022a

A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples

Auteurs: Amaral C, Antunes W, Moe E, Duarte AG, Lima LMP, Santos C, Gomes IL, Afonso GS, Vieira R, Teles HSS, Reis MS, da Silva MAR, Henriques AM, Fevereiro M, Ventura MR, Serrano M, Pimentel C.
Publié dans: Scientific Reports, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-95799-6

Crossing the Wall: Characterization of the Multiheme Cytochromes Involved in the Extracellular Electron Transfer Pathway of Thermincola ferriacetica.

Auteurs: Faustino MM, Fonseca BM, Costa NL, Lousa D, Louro RO, Paquete CM
Publié dans: Microorganisms, 2021, ISSN 2076-2607
Éditeur: Basel MDPI AG 2013
DOI: 10.3390/microorganisms9020293

Resonance Raman spectroscopic analysis of the iron–sulfur cluster redox chain of the Ralstonia eutropha membrane-bound [NiFe]-hydrogenase

Auteurs: Siebert, E., Schmidt, A., Frielingsdorf, S., Kalms, J., Kuhlmann, U., Lenz, O., Scheerer, P., Zebger, I., Hildebrandt, P.
Publié dans: Journal of Raman Spectroscopy, 2021, ISSN 0377-0486
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jrs.6163

The large subunit of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha - a minimal hydrogenase?

Auteurs: Caserta G, Lorent C, Ciaccafava A, Keck M, Breglia R, Greco C, Limberg C, Hildebrandt P, Cramer SP, Zebger I, Lenz O.
Publié dans: Chemical Science, 2020, ISSN 2041-6520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0sc01369b

Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives.

Auteurs: Lopes RR, Tomé CS, Russo R, Paterna R, Leandro J, Candeias NR, Gonçalves LMD, Teixeira M, Sousa PMF, Guedes RC, Vicente JB, Gois PMP, Leandro P.
Publié dans: Biomolecules, 2021, ISSN 2218-273X
Éditeur: MDPI AG
DOI: 10.3390/biom11030462

Redox-Polymer-Wired [NiFeSe] Hydrogenase Variants with Enhanced O2 Stability for Triple-Protected High-Current-Density H2 -Oxidation Bioanodes

Auteurs: Ruff A, Szczesny J, Vega M, Zacarias S, Matias PM, Gounel S, Mano N, Pereira IAC, Schuhmann W.
Publié dans: ChemSusChem, 2020, ISSN 1864-564X
Éditeur: ChemSusChem
DOI: 10.1002/cssc.202000999

Repair of Iron Center Proteins-A Different Class of Hemerythrin-like Proteins

Auteurs: Silva LSO, Matias PM, Romão CV, Saraiva LM.
Publié dans: Molecules, Numéro 14203049, 2022, ISSN 1420-3049
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27134051

Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases.

Auteurs: Silveira CM, Moe E, Fraaije M, Martins LO, Todorovic S.
Publié dans: RSC advances, 2020, ISSN 2046-2069
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0ra00950d

Molecular Details on Multiple Cofactor Containing Redox Metalloproteins Revealed by Infrared and Resonance Raman Spectroscopies

Auteurs: Silveira CM, Zuccarello L, Barbosa C, Caserta G, Zebger I, Hildebrandt P, Todorovic S
Publié dans: Molecules, 2021, ISSN 1420-3049
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26164852

How the anaerobic enteropathogen clostridioides difficile tolerates low O2 tensions

Auteurs: Kint N, Alves Feliciano C, Martins MC, Morvan C, Fernandes SF, Folgosa F, Dupuy B, Texeira M, Martin-Verstraete I
Publié dans: mBio, 2020, ISSN 2161-2129
Éditeur: American Society for Microbiology
DOI: 10.1128/mbio.01559-20

PRE-driven protein NMR structures: an alternative approach in highly paramagnetic systems

Auteurs: Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M.
Publié dans: FEBS Journal, 2021, ISSN 1742-4658
Éditeur: Federation of European Biochemical Societies journal
DOI: 10.1111/febs.15615

An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity

Auteurs: Amaral C, Vicente CT, Caetano SM, Gaspar-Cordeiro A, Yang Y, Cloetens P, Romão CV, Rodrigues-Pousada C, Pimentel C
Publié dans: Microorganisms, Numéro 20762607, 2021, ISSN 2076-2607
Éditeur: Basel MDPI AG 2013
DOI: 10.3390/microorganisms9061337

SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices

Auteurs: Zuccarello L, Barbosa C, Galdino E, Lončar N, Silveira CM, Fraaije MW, Todorovic S
Publié dans: Int J Mol Sci., Numéro 14220067, 2021, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22157998

Sequence-specific Assignments in NMR Spectra of paramagnetic systems: A non-systematic approach,

Auteurs: Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M.
Publié dans: Inorg. Chim. Acta, Numéro 00201693, 2021, ISSN 0020-1693
Éditeur: Elsevier BV
DOI: 10.1016/j.ica.2020.119984

DsrL mediates electron transfer between NADH and rDsrAB in Allochromatium vinosum.

Auteurs: Löffler M, Feldhues J, Venceslau SS, Kammler L, Grein F, Pereira IAC, Dahl C
Publié dans: Environmental Microbiology, 2020, ISSN 1462-2912
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14899

Measuring transverse relaxation in highly paramagnetic systems

Auteurs: Invernici M, Trindade IB, Cantini F, Louro RO, Piccioli M.
Publié dans: J Biomol NMR, Numéro 09252738, 2020, ISSN 0925-2738
Éditeur: Kluwer Academic Publishers
DOI: 10.1007/s10858-020-00334-w

Integration of a Hydrogenase in a Lead Halide Perovskite Photoelectrode for Tandem Solar Water Splitting

Auteurs: Edwardes Moore E, Andrei V, Zacarias S, Pereira IAC, Reisner E
Publié dans: ACS Energy Letters, Numéro 23808195, 2019, ISSN 2380-8195
Éditeur: American Chemical Society
DOI: 10.1021/acsenergylett.9b02437

Responses of Clostridia to oxygen: from detoxification to adaptive strategies

Auteurs: Morvan C, Folgosa F, Kint N, Teixeira M, Martin-Verstraete I.
Publié dans: Environmental Microbiology, Numéro 14622912, 2021, ISSN 1462-2912
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15665

Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-c Maturation System

Auteurs: Silva AV, Firmino MO, Costa NL, Louro RO, Paquete CM
Publié dans: Biomolecules, 2022, ISSN 2218-273X
Éditeur: MDPI AG
DOI: 10.3390/biom12040549

Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis.

Auteurs: Silva LSO, Matias PM, Romão CV, Saraiva LM.
Publié dans: Frontiers in Microbiology, 2021, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2021.670681

In Vitro Assembly as a Tool to Investigate Catalytic Intermediates of [NiFe]-Hydrogenase.

Auteurs: Caserta G, Lorent C, Pelmenschikov V, Schoknecht J, Yoda Y, Hildebrandt P, Cramer SP, Zebger I, Lenz O
Publié dans: ACS Catalysis, 2020, ISSN 2155-5435
Éditeur: American Chemical Society
DOI: 10.1021/acscatal.0c04079

Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1

Auteurs: Silva AV, Edel M, Gescher J, Paquete CM
Publié dans: Frontiers in Microbiology, 2020, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2020.00815

Sulfide and Carbon Monoxide Tolerance in Bacteria

Auteurs: Silva LSO, Matias PM, Romão CV, Saraiva LM
Publié dans: Antioxidants, 2021, ISSN 2076-3921
Éditeur: MDPI AG
DOI: 10.3390/antiox10050729

Staphylococcus aureus flavohaemoglobin contributes to early stage biofilm development under nitrosative stress

Auteurs: Carvalho SM, Freitas CS, Oliveira AS, Saraiva LM
Publié dans: FEMS Microbiology Letters, 2021, ISSN 0378-1097
Éditeur: Blackwell
DOI: 10.1093/femsle/fnab131

Disentangling Unusual Catalytic Properties and the Role of the [4Fe-4S] Cluster of Three Endonuclease III from the Extremophile D. radiodurans

Auteurs: Rollo, F.; Borges, P.T.; Silveira, C.M.; Rosa, M.T.G.; Todorovic, S.; Moe, E.
Publié dans: Molecules, 2022, ISSN 1420-3049
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27134270

The Amino Acids Motif -32GSSYN36- in the Catalytic Domain of E. coli Flavorubredoxin NO Reductase Is Essential for Its Activity

Auteurs: Martins, M.C.; Fernandes, S.F.; Salgueiro, B.A.; Soares, J.C.; Romão, C.V.; Soares, C.M.; Lousa, D.; Folgosa, F.; Teixeira, M
Publié dans: Catalysts, Numéro 20734344, 2021, ISSN 2073-4344
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal11080926

Role of multiheme cytochromes involved in extracellular anaerobic respiration in bacteria

Auteurs: Edwards MJ, Richardson DJ, Paquete CM, Clarke TA
Publié dans: Protein Science, 2020, ISSN 0961-8368
Éditeur: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3787

Fast CO2 hydration kinetics impair heterogeneous but improve enzymatic CO2reduction catalysis

Auteurs: Cobb SJ, Badiani VM, Dharani AM, Wagner A, Zacarias S, Oliveira AR, Pereira IAC, Reisner E.
Publié dans: Nature Chemistry, Numéro 17554330, 2022, ISSN 1755-4330
Éditeur: Nature Publishing Group
DOI: 10.1038/s41557-021-00880-2

The Di-iron RIC Protein (YtfE) of Escherichia coli Interacts with the DNA-Binding Protein from Starved Cells (Dps) To Diminish RIC Protein-Mediated Redox Stress

Auteurs: Liliana S. O. Silva, Joana M. Baptista, Charlotte Batley, Simon C. Andrews, Lígia M. Saraiva
Publié dans: Journal of Bacteriology, Numéro 200/24, 2018, ISSN 0021-9193
Éditeur: American Society for Microbiology
DOI: 10.1128/jb.00527-18

An electrogenic redox loop in sulfate reduction reveals a likely widespread mechanism of energy conservation

Auteurs: Américo G. Duarte, Teresa Catarino, Gaye F. White, Diana Lousa, Sinje Neukirchen, Cláudio M. Soares, Filipa L. Sousa, Thomas A. Clarke, Inês A. C. Pereira
Publié dans: Nature Communications, Numéro 9/1, 2018, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-018-07839-x

Identification of the sirohaem biosynthesis pathway in Staphylococcus aureus

Auteurs: Marco A. M. Videira, Susana A. L. Lobo, Filipa L. Sousa, Lígia M. Saraiva
Publié dans: The FEBS Journal, 2019, ISSN 1742-464X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/febs.15091

The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

Auteurs: Sandra P. Santos, Yang Yang, Margarida T. G. Rosa, Mafalda A. A. Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A. Carrondo, Peter Cloetens, Isabel A. Abreu, Célia V. Romão
Publié dans: Scientific Reports, Numéro 9/1, 2019, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-019-53140-2

Metabolomics of Escherichia coli Treated with the Antimicrobial Carbon Monoxide-Releasing Molecule CORM-3 Reveals Tricarboxylic Acid Cycle as Major Target

Auteurs: Sandra M. Carvalho, Joana Marques, Carlos C. Romão, Lígia M. Saraiva
Publié dans: Antimicrobial Agents and Chemotherapy, Numéro 63/10, 2019, ISSN 0066-4804
Éditeur: American Society for Microbiology
DOI: 10.1128/aac.00643-19

Interfacing Formate Dehydrogenase with Metal Oxides for the Reversible Electrocatalysis and Solar-Driven Reduction of Carbon Dioxide

Auteurs: Melanie Miller, William E. Robinson, Ana Rita Oliveira, Nina Heidary, Nikolay Kornienko, Julien Warnan, Inês A. C. Pereira, Erwin Reisner
Publié dans: Angewandte Chemie International Edition, Numéro 58/14, 2019, Page(s) 4601-4605, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201814419

How Thermophilic Gram-Positive Organisms Perform Extracellular Electron Transfer: Characterization of the Cell Surface Terminal Reductase OcwA

Auteurs: N. L. Costa, B. Hermann, V. Fourmond, M. M. Faustino, M. Teixeira, O. Einsle, C. M. Paquete, R. O. Louro
Publié dans: mBio, Numéro 10/4, 2019, ISSN 2150-7511
Éditeur: American Society for microbiology
DOI: 10.1128/mbio.01210-19

How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes

Auteurs: Maria C. Martins, Célia V. Romão, Filipe Folgosa, Patrícia T. Borges, Carlos Frazão, Miguel Teixeira
Publié dans: Free Radical Biology and Medicine, Numéro 140, 2019, Page(s) 36-60, ISSN 0891-5849
Éditeur: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2019.01.051

Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition

Auteurs: Veronica Palma Delgado, Catarina M. Paquete, Gunnar Sturm, Johannes Gescher
Publié dans: Bioelectrochemistry, Numéro 129, 2019, Page(s) 18-25, ISSN 1567-5394
Éditeur: Elsevier BV
DOI: 10.1016/j.bioelechem.2019.04.022

Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility

Auteurs: Inês B. Trindade, José M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro
Publié dans: Journal of Biological Chemistry, Numéro 294/1, 2019, Page(s) 157-167, ISSN 0021-9258
Éditeur: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.005041

Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic

Auteurs: Katarzyna P. Sokol, William E. Robinson, Ana R. Oliveira, Sonia Zacarias, Chong-Yong Lee, Christopher Madden, Arnau Bassegoda, Judy Hirst, Inês A. C. Pereira, Erwin Reisner
Publié dans: Journal of the American Chemical Society, Numéro 141/44, 2019, Page(s) 17498-17502, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.9b09575

A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase

Auteurs: Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira
Publié dans: ACS Catalysis, Numéro 9/9, 2019, Page(s) 8509-8519, ISSN 2155-5435
Éditeur: American Chemical Society
DOI: 10.1021/acscatal.9b02347

Photoreduction of CO 2 with a Formate Dehydrogenase Driven by Photosystem II Using a Semi-artificial Z-Scheme Architecture

Auteurs: Katarzyna P. Sokol, William E. Robinson, Ana R. Oliveira, Julien Warnan, Marc M. Nowaczyk, Adrian Ruff, Inês A. C. Pereira, Erwin Reisner
Publié dans: Journal of the American Chemical Society, Numéro 140/48, 2018, Page(s) 16418-16422, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.8b10247

Optimizing Electroactive Organisms: The Effect of Orthologous Proteins

Auteurs: Bruno M. Fonseca, Luís Silva, Inês B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete
Publié dans: Frontiers in Energy Research, Numéro 7, 2019, ISSN 2296-598X
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fenrg.2019.00002

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells

Auteurs: Karim Zuhra, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, Alessandro Giuffrè
Publié dans: Cells, Numéro 8/8, 2019, Page(s) 828, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells8080828

Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode

Auteurs: Estelle Lebègue, Nazua L. Costa, Bruno M. Fonseca, Ricardo O. Louro, Frédéric Barrière
Publié dans: Journal of Electroanalytical Chemistry, Numéro 847, 2019, Page(s) 113232, ISSN 1572-6657
Éditeur: Elsevier BV
DOI: 10.1016/j.jelechem.2019.113232

Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery

Auteurs: Veronica Nasta, Stefano Da Vela, Spyridon Gourdoupis, Simone Ciofi-Baffoni, Dmitri I. Svergun, Lucia Banci
Publié dans: Scientific Reports, Numéro 9/1, 2019, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-019-55313-5

Methylglyoxal interaction with superoxide dismutase 1

Auteurs: Panagis Polykretis, Enrico Luchinat, Francesca Boscaro, Lucia Banci
Publié dans: Redox Biology, Numéro 30, 2020, Page(s) 101421, ISSN 2213-2317
Éditeur: Elsevier BV
DOI: 10.1016/j.redox.2019.101421

X-ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases

Auteurs: Yulia Ilina, Christian Lorent, Sagie Katz, Jae-Hun Jeoung, Seigo Shima, Marius Horch, Ingo Zebger, Holger Dobbek
Publié dans: Angewandte Chemie International Edition, Numéro 58/51, 2019, Page(s) 18710-18714, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201908258

NMR of paramagnetic metalloproteins in solution: Ubi venire, quo vadis?

Auteurs: Trindade IB, Coelho A, Cantini F, Piccioli M, Louro RO.
Publié dans: J Inorg Biochem, Numéro 01620134, 2022, ISSN 0162-0134
Éditeur: Elsevier BV
DOI: 10.1016/j.jinorgbio.2022.111871

A New Paradigm of Multiheme Cytochrome Evolution by Grafting and Pruning Protein Modules

Auteurs: Soares R, Costa NL, Paquete CM, Andreini C, Louro RO
Publié dans: Mol Biol Evol, Numéro 15371719, 2022, ISSN 1537-1719
Éditeur: Oxford University Press
DOI: 10.1093/molbev/msac139

Synergetic Antimicrobial Activity and Mechanism of Clotrimazole- Linked CO-Releasing Molecules

Auteurs: Sofia S. Mendes, Joana Marques, Edit Mesterházy, Jan Straetener, Melina Arts, Teresa Pissarro, Jorgina Reginold, Anne Berscheid, Jan Bornikoel, Robert M. Kluj, Christoph Mayer, Filipp Oesterhelt, Sofia Friães, Beatriz Royo, Tanja Schneider, Heike Brötz-Oesterhelt, Carlos C. Romão, and Lígia M. Saraiva
Publié dans: ACS Bio & Med Chem Au, Numéro 26942437, 2022, ISSN 2694-2437
Éditeur: American Chemical Society
DOI: 10.1021/acsbiomedchemau.2c00007

Let's chat: Communication between electroactive microorganisms

Auteurs: Paquete CM, Rosenbaum MA, Bañeras L, Rotaru AE, Puig S.
Publié dans: Bioresource Technology, 2022, ISSN 0960-8524
Éditeur: Elsevier BV
DOI: 10.1016/j.biortech.2022.126705

The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase

Auteurs: Ferreira D, Barbosa ACC, Oliveira GP, Catarino T, Venceslau SS, Pereira IAC
Publié dans: PNAS, 2022, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.2118880119

Structural and spectroscopic characterization of a HdrA-like subunit from Hyphomicrobium denitrificans

Auteurs: Ernst C, Kayastha K, Koch T, Venceslau SS, Pereira IAC, Demmer U, Ermler U, Dahl C.
Publié dans: FEBS Journal, 2021, ISSN 1742-464X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/febs.15505

Unusual structures and unknown roles of Fes clusters in metalloenzymes seen from a resonance Raman Spectroscopic Perspective

Auteurs: Caserta, G.; Zuccarello, L.; Barbosa, C.; Silveira, C. M.; Moe, E.; Katz, S.; Hildebrandt, P.; Zebger, I.; Todorovic, S.
Publié dans: Coordination Chemistry Reviews, 2022, ISSN 0010-8545
Éditeur: Elsevier BV
DOI: 10.1016/j.ccr.2021.214287

In Vitro Assembly Exploring Structure and Function of Redox Intermediates in [NiFe]-Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes

Auteurs: C. Lorent, V. Pelmenschikov, S. Frielingsdorf, J. Schoknecht, G. Caserta, Y. Yoda, H. Wang, K. Tamasaku, O. Lenz, S.P. Cramer, M. Horch, L. Lauterbach and I. Zebger
Publié dans: Angewandte Chemie Int, 2021, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202100451

Mutational and structural analysis of an ancestral fungal dye-decolorizing peroxidase

Auteurs: Zitare UA, Habib MH, Rozeboom H, Mascotti ML, Todorovic S, Fraaije MW.
Publié dans: FEBS Journal, 2021, ISSN 1742-464X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/febs.15687

Unique Biradical Intermediate in the Mechanism of the Heme Enzyme Chlorite Dismutase

Auteurs: Püschmann J, Mahor D, de Geus DC, Strampraad MJF, Srour B, Hagen WR, Todorovic S, Hagedoorn PL
Publié dans: ACS Catalysis, 2021, ISSN 2155-5435
Éditeur: American Chemical Society
DOI: 10.1021/acscatal.1c03432

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Auteurs: Zuccarello, L.; Barbosa, C.; Todorovic, S.; Silveira, C.M.
Publié dans: Catalysts, 2021, ISSN 2073-4344
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal11020218

Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli.

Auteurs: Fan Q, Caserta G, Lorent C, Lenz O, Neubauer P, Gimpel M.
Publié dans: Microorganisms, 2021, ISSN 2076-2607
Éditeur: MDPI
DOI: 10.3390/microorganisms9061195

Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing

Auteurs: Barbosa C, Silveira CM, Silva D, Brissos V, Hildebrandt P, Martins LO, Todorovic S
Publié dans: Biosensors and bioelectronics, 2020, ISSN 0956-5663
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2020.112055

Copper Acts Synergistically With Fluconazole in Candida glabrata by Compromising Drug Efflux, Sterol Metabolism, and Zinc Homeostasis.

Auteurs: Gaspar-Cordeiro A, Amaral C, Pobre V, Antunes W, Petronilho A, Paixão P, Matos AP, Pimentel C.
Publié dans: Frontiers in Microbiology, Numéro 1664302X, 2022, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2022.920574

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