Breaking Bad Biofilms. Innovative Analysis and Design Rules for Next-Generation Antifouling Interfaces

Project Information

BREAK BIOFILMS

Grant agreement ID: 813439

DOI 10.3030/813439

Project closed

EC signature date 20 August 2018

Start date 1 January 2019

End date 30 April 2023

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 4 033 858,32

EU contribution € 4 033 858,32

Coordinated by

UNIVERSIDAD DE OVIEDO
Spain

Deliverables

Documents, reports (12)

Highly efficient nano/micro-textured surfaces for efficient pathogen capture >80% at 100 CFU/ml.

Highly efficient nanomicrotextured surfaces for efficient pathogen capture 80 at 100 CFUml

Post-project exploitation plan

Postproject exploitation plan

Generic scheme for Virtual Company concept transferable to other ITN projects
"Business Plan of the ESR ""virtual company"""

Business Plan of the ESR virtual company

3rd annual report
1st annual report
Report on Gender balance and gender case studies
2nd annual report
"Report on ""in vitro"" toxicity of the anti-biofilm novel formulations"

Report on in vitro toxicity of the antibiofilm novel formulations

Proceedings volume of final Breaking Biofilms Conference
Understanding of chemical processes within biofilms
Disposable paper microfluidic platforms for the study of biofilm activity and biocide high throughput screening

Websites, patent fillings, videos etc. (1)

Project website established

Other (1)

Establishment of social media presence

Publications

Peer reviewed articles (38)

Draft genome sequences of the bap-producing strain staphylococcus aureus v329 and its derived phage-resistant mutant bim-1

Author(s): Lucía Fernández, Ana Catarina Duarte, Beatriz Martínez, Ana Rodríguez, Pilar García
Published in: Microbiology Resource Announcements, 2021, ISSN 2576-098X
Publisher: American Society for Microbiology
DOI: 10.1128/mra.00500-21

Fe3O4@Au Core–Shell Magnetic Nanoparticles for the Rapid Analysis of E. coli O157:H7 in an Electrochemical Immunoassay

Author(s): Shayesteh Bazsefidpar, Maria Freitas, Clara R. Pereira, Gemma Gutiérrez, Esther Serrano-Pertierra, Henri P. A. Nouws, María Matos, Cristina Delerue-Matos and María Carmen Blanco-López
Published in: Biosensors, Issue 13, 2023, Page(s) 567, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios13050567

Vesicles as antibiotic carrier: State of art

Author(s): Verdiana Marchianò, María Matos, Esther Serrano-Pertierra, Gemma Gutiérrez, M.C. Blanco-López
Published in: International Journal of Pharmaceutics, Issue 585, 2020, Page(s) 119478, ISSN 0378-5173
Publisher: Elsevier BV
DOI: 10.1016/j.ijpharm.2020.119478

Electrochemical assays for microbial analysis: how far they are from solving microbiota and microbiome challenges

Author(s): Elena E. Ferapontova
Published in: Current Opinion in Electrochemistry, Issue 19, 2020, Page(s) 153-161, ISSN 2451-9103
Publisher: Elsevier B.V.
DOI: 10.1016/j.coelec.2019.12.005

Electrochemical immuno-and aptamer-based assays for bacteria: Pros and cons over traditional detection schemes

Author(s): Rimsha Binte Jamal, Stepan Shipovskov, Elena E. Ferapontova
Published in: Sensors, 2020, ISSN 1424-8220
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s20195561

Gram-Positive Pneumonia: Possibilities Offered by Phage Therapy

Author(s): Lucía Fernández, María Dolores Cima-Cabal, Ana Catarina Duarte, Ana Rodríguez, María del Mar García-Suárez and Pilar García
Published in: Antibiotics, 2021, ISSN 2079-6382
Publisher: MDPI
DOI: 10.3390/antibiotics10081000

Stability of non-ionic surfactant vesicles loaded with Rifamycin S

Author(s): Verdiana Marchianò, Maria Matos, Ismael Marcet, Maria Paz Cabal, Gemma Gutiérrez and Maria Carmen Blanco-López
Published in: Pharmaceutics, 2022, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics14122626

ZnO Nanostructures with Antibacterial Properties Prepared by a Green Electrochemical-Thermal Approach

Author(s): Maria Chiara Sportelli, Rosaria Anna Picca, Margherita Izzi, Gerardo Palazzo, Roberto Gristina, Massimo Innocenti, Luisa Torsi, Nicola Cioffi
Published in: Nanomaterials, Issue 10/3, 2020, Page(s) 473, ISSN 2079-4991
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nano10030473

In situ monitoring of Lentilactobacillus parabuchneri biofilm formation via real-time infrared spectroscopy

Author(s): Diellza Bajrami, Stephan Fischer, Holger Barth, María A. Sarquis, Victor M. Ladero, María Fernández, Maria. C. Sportelli, Nicola Cioffi, Christine Kranz and Boris Mizaikoff
Published in: npj Biofilms and Microbiomes, 2022, ISSN 2055-5008
Publisher: Springer Nature
DOI: 10.1038/s41522-022-00353-5

Green Synthesis and Characterization of Antimicrobial Synergistic AgCl/BAC Nanocolloids

Author(s): Syed Imdadul Hossain, Maria Chiara Sportelli, Rosaria Anna Picca, Luigi Gentile, Gerardo Palazzo, Nicoletta Ditaranto, and Nicola Cioffi
Published in: ACS Applied bio materials, 2022, ISSN 2576-6422
Publisher: American Chemical Society (ACS)
DOI: 10.1021/acsabm.2c00207

Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

Author(s): Kukushkina, E.A.; Mateos, H.; Altun, N.; Sportelli, M.C.; Gonzalez, P.; Picca, R.A.; Cioffi, N.
Published in: Antibiotics, 2022, ISSN 2079-6382
Publisher: MDPI
DOI: 10.3390/antibiotics11101396

Electrochemical Preparation of Synergistic Nanoantimicrobials

Author(s): Maria Chiara Sportelli, Daniela Longano, Elisabetta Bonerba, Giuseppina Tantillo, Luisa Torsi, Luigia Sabbatini, Nicola Cioffi, Nicoletta Ditaranto
Published in: Molecules, Issue 25/1, 2020, Page(s) 49, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25010049

Lyophilised nanovesicles loaded with vitamin B12

Author(s): Verdiana Marchianò, Maria Matos, Esther Serrano, José R. Álvarez, Ismael Marcet, M. Carmen Blanco-López, Gemma Gutiérrez
Published in: Journal of Molecular Liquids, 2022, ISSN 0167-7322
Publisher: Elsevier BV
DOI: 10.1016/j.molliq.2022.120129

Probing and Visualizing Interfacial Charge at Surfaces in Aqueous Solution

Author(s): Giada Caniglia, Gözde Tezcan, Gabriel N. Meloni, Patrick R. Unwin, Christine Kranz
Published in: Annual Review of Analytical Chemistry, 2022, ISSN 1936-1327
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-anchem-121521-122615

Atomic force and infrared spectroscopic studies on the role of surface charge for the anti-biofouling properties of polydopamine films

Author(s): Giada Caniglia, Andrea Teuber, Holger Barth, Boris Mizaikoff, Christine Kranz
Published in: Analytical and Bioanalytical Chemistry, 2023, ISSN 1618-2642
Publisher: Springer Verlag
DOI: 10.1007/s00216-022-04431-7

Electrochemical detection of viruses and antibodies: A mini review

Author(s): Miren Ruiz de Eguilaz, Loanda R. Cumba, Robert J.Forster
Published in: Electrochemistry Communications, 2020, ISSN 1388-2481
Publisher: Elsevier BV
DOI: 10.1016/j.elecom.2020.106762

Cu Nanoparticle-Loaded Nanovesicles with Antibiofilm Properties. Part I: Synthesis of New Hybrid Nanostructures

Author(s): Lucia Sarcina, Pablo García-Manrique, Gemma Gutiérrez, Nicoletta Ditaranto, Nicola Cioffi, Maria Matos, Maria del Carmen Blanco-López
Published in: Nanomaterials, Issue 10/8, 2020, Page(s) 1542, ISSN 2079-4991
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nano10081542

Detection of E.coli 23S rRNA by electrocatalytic “off-on” DNA beacon assay with femtomolar sensitivity

Author(s): Rimsha B. Jamal, Toni Vitasovic, Ulrich Gosewinkel, Elena E. Ferapontova
Published in: Biosensors and Bioelectronics, 2023, ISSN 0956-5663
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2023.115214

Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings.

Author(s): Giada Caniglia; Christine Kranz
Published in: Analytical and Bioanalytical Chemistry, Issue 1, 2020, ISSN 1618-2642
Publisher: Springer Verlag
DOI: 10.1007/s00216-020-02782-7

Design and Selection of Engineered Lytic Proteins With Staphylococcus aureus Decolonizing Activity

Author(s): Diana Gutiérrez, Lorena Rodríguez-Rubio, Patricia Ruas-Madiedo, Lucía Fernández, Ana Belén Campelo, Yves Briers, Martin Weiss Nielsen, Karl Pedersen, Rob Lavigne, Pilar García and Ana Rodríguez
Published in: Frontiers in Microbiology, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.723834

Synergistic action of phage phiIPLA-RODI and lytic protein CHAPSH3b: a combination strategy to target Staphylococcus aureus biofilms

Author(s): Ana Catarina Duarte, Lucía Fernández, Vincent De Maesschalck, Diana Gutiérrez, Ana Belén Campelo, Yves Briers, Rob Lavigne, Ana Rodríguez & Pilar García
Published in: npj Biofilms and Microbiomes, 2021, ISSN 2055-5008
Publisher: Nature Publishing Group
DOI: 10.1038/s41522-021-00208-5

Combined use of bacteriocins and bacteriophages as food biopreservatives. A review

Author(s): Claudia Rendueles; Ana Catarina Duarte; Susana Escobedo; Lucía Fernández; Ana Rodríguez; Pilar García; Beatriz Martínez
Published in: International Journal of Food Microbiology, 2022, ISSN 0168-1605
Publisher: Elsevier BV
DOI: 10.1016/j.ijfoodmicro.2022.109611

Can Nanotechnology and Materials Science Help the Fight against SARS-CoV-2?

Author(s): Maria Chiara Sportelli, Margherita Izzi, Ekaterina A. Kukushkina, Syed Imdadul Hossain, Rosaria Anna Picca, Nicoletta Ditaranto, Nicola Cioffi
Published in: Nanomaterials, 2020, ISSN 2079-4991
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nano10040802

Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement

Author(s): Shayesteh Bazsefidpar, Esther Serrano‑Pertierra, Gemma Gutiérrez, Alberto Sánchez Calvo, María Matos, María Carmen Blanco‑López
Published in: Microchimica Acta, 2023, ISSN 0026-3672
Publisher: Springer Verlag
DOI: 10.1007/s00604-023-05834-8

Preparation of Laser-Ablated Ag Nanoparticle–MMT Clay-Based Beeswax Antibiofilm Coating

Author(s): Hossain, S.I.; Bajrami, D.; Sportelli, M.C.; Picca, R.A.; Volpe, A.; Gaudiuso, C.; Ancona, A.; Gentile, L.; Palazzo, G.; Ditaranto, N.; Mizaikoff, B.; Cioffi, N
Published in: Antibiotics, 2023, ISSN 2079-6382
Publisher: MDPI
DOI: 10.3390/antibiotics12020194

The relationship between the phageome and human health: Are bacteriophages beneficial or harmful microbes?

Author(s): Fernández Llamas, Lucía; Duarte, Ana Catarina; Rodríguez González, Ana; García Suárez, María Pilar
Published in: Beneficial Microbes, 2021, ISSN 1876-2883
Publisher: Wageningen Academic Publishers
DOI: 10.3920/bm2020.0132

Electrodecoration and Characterization of Superparamagnetic Iron Oxide Nanoparticles with Bioactive Synergistic Nanocopper: Magnetic Hyperthermia-Induced Ionic Release for Anti-Biofilm Action

Author(s): Verdiana Marchianò, Maria Salvador, Amanda Moyano, Gemma Gutiérrez, María Matos, Susana Yáñez-Vilar, Yolanda Piñeiro, José Rivas, José C. Martínez-García, Davide Peddis, Maria C. Blanco-López, Montserrat Rivas, Nicoletta Ditaranto, Nicola Cioffi
Published in: Antibiotics, Issue 10/2, 2021, Page(s) 119, ISSN 2079-6382
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/antibiotics10020119

Electrochemical ozone generation using compacted high pressure high temperature synthesized boron doped diamond microparticle electrodes

Author(s): Georgia F. Wood; Irina M. Terrero Rodríguez; Joshua J. Tully; Shayantan Chaudhuri; Julie V. Macpherson
Published in: Journal of The Electrochemical Society, 2021, ISSN 0013-4651
Publisher: Electrochemical Society, Inc.
DOI: 10.1149/1945-7111/ac3ff4

Sustainable antibiofilm self-assembled colloidal systems

Author(s): Diana Morán, Clara Saweres-Argüelles, Verdiana Marchiano, Shayesteh Bazsefidpar, Esther Serrano-Pertierra, Maria Matos, Gemma Gutierrez and Maria Carmen Blanco-López
Published in: Frontiers in Soft Matter, Issue 2, 2022, ISSN 2813-0499
Publisher: Frontiers Media
DOI: 10.3389/frsfm.2022.1041881

Nanovesicles as vanillin carriers for antimicrobial applications

Author(s): Verdiana Marchianò, Maria Matos, Miriam López, Shihan Weng, Esther Serrano-Pertierra, Susana Luque, M. Carmen Blanco-López, Gemma Gutiérrez
Published in: Membranes, 2023, ISSN 2077-0375
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/membranes13010095

Recent advances on the spectroscopic characterization of microbial biofilms: A critical review

Author(s): Sportelli, Maria Chiara; Kranz, Christine; Mizaikoff, Boris; Cioffi, Nicola
Published in: Analytica Chimica Acta, 2022, ISSN 0003-2670
Publisher: Elsevier BV
DOI: 10.1016/j.aca.2022.339433

Developing Diagnostic and Therapeutic Approaches to Bacterial Infections for a New Era: Implications of Globalization

Author(s): Lucía Fernández, María Dolores Cima-Cabal, Ana Catarina Duarte, Ana Rodriguez, Pilar García, María del Mar García-Suárez
Published in: Antibiotics, Issue 9/12, 2020, Page(s) 916, ISSN 2079-6382
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/antibiotics9120916

Ag-Based Synergistic Antimicrobial Composites. A Critical Review

Author(s): Ekaterina A. Kukushkina, Syed Imdadul Hossain, Maria Chiara Sportelli, Nicoletta Ditaranto, Rosaria Anna Picca, Nicola Cioffi
Published in: Nanomaterials, Issue 11/7, 2021, Page(s) 1687, ISSN 2079-4991
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nano11071687

Lipid–polymer hybrids encapsulating iron-oxide nanoparticles as a label for lateral flow immunoassays

Author(s): Shayesteh Bazsefidpar, Amanda Moyano Artime, Gemma Gutiérrez Cervelló, María Matos González, María del Carmen Blanco López
Published in: Biosensors, 2021, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/bios11070218

Characterization of the Biofilms Formed by Histamine-Producing Lentilactobacillus parabuchneri Strains in the Dairy Environment

Author(s): Agustina Sarquis, Diellza Bajrami, Boris Mizaikoff, Victor Ladero, Miguel A Alvarez, Maria Fernandez
Published in: Foods, Issue 12, 2023, Page(s) 1503, ISSN 2304-8158
Publisher: Multidisciplinary Digital Publishing Institute
DOI: 10.3390/foods12071503

Self-Standing Bioinspired Polymer Films Doped with Ultrafine Silver Nanoparticles as Innovative Antimicrobial Material

Author(s): Kukushkina, E.A; Tartaro, G.;.Sportelli, M.C.; Di Franco, C.; Picca, R.A.; Cioffi, N.
Published in: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232415818

Silver-fluoropolymer (Ag-CFx) films: Kinetic study of silver release, and spectroscopic-microscopic insight into the inhibition of P. fluorescens biofilm formation

Author(s): Giada Caniglia; Maria Chiara Sportelli; Anna Heinzmann; Rosaria A. Picca; Antonio Valentini; Holger Barth; Boris Mizaikoff; Nicola Cioffi; Christine Kranz
Published in: Analytica Chimica Acta, 2022, ISSN 0003-2670
Publisher: Elsevier BV
DOI: 10.1016/j.aca.2022.339892

A Review on Montmorillonite-Based Nanoantimicrobials: State of the Art

Author(s): Hossain, S.I.; Kukushkina, E.A.; Izzi, M.; Sportelli, M.C.; Picca, R.A.; Ditaranto, N.; Cioffi, N.
Published in: Nanomaterials, 2023, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano13050848

Book chapters (1)

Miniaturized Electrochemical Biosensors

Author(s): Andreas Hellmann, Giada Caniglia, Christine Kranz
Published in: Encyclopedia of Sensors and Biosensors, 2023
Publisher: Elsevier
DOI: 10.1016/b978-0-12-822548-6.00152-7