Deliverables Documents, reports (4) Optimisation of the production process for CAR T cells dose-efficacy correlation Optimisation of the production process for CAR T cells and understanding of the doseefficacy correlation Effect of different protein product types on productivity and glycan heterogeneity in CHO cells Understanding of the effect of different protein product types on productivity and glycan heterogeneity in CHO cells Cell biology during extended HCDP culture Understanding of the underlying cell biology during extended HCDP culture Rhapsody scRNASeq, mtDNA and proteomics of cell therapies Rhapsody scRNASeq mtDNA and proteomics of cell therapies complete Websites, patent fillings, videos etc. (4) Manuscripts submitted to peer-reviewed journals (i.e. peer reviewed primary research papers to be published after publication by journal) Papers on STACCATO research results will be submitted to open access peerreviewed journals For nonopen access publications articles will be deposited in an online repository to be made accessible STACCATO Podcast Seminar Series Biannual seminars to be broadcasted via FacebookLive Each seminar will be published via the STACCATO podcast on the project website Conference presentations and posters Participation of ESRs and PIs at relecant scientific conferences to disseminate the latest project results STACCATO Website The project website of STACCATO will have the main purpose of illustrating the application of single cell analysis for bioprocess development to the general public Additionally it will be the main platform for disseminating resarch project results and informing about the project in general Publications Peer reviewed articles (14) Proof-of-Concept of Continuous Transfection for Adeno-Associated Virus Production in Microcarrier-Based Culture Author(s): Brian Ladd, Kevin Bowes, Mats Lundgren, Torbjörn Gräslund and Veronique Chotteau Published in: Processes, 2022, 10(3), 515, 2022, ISSN 2227-9717 Publisher: MDPI DOI: 10.3390/pr10030515 Impact of dual baculovirus infection on the Sf9 insect cell transcriptome during AAV production using single-cell RNA-seq Author(s): Virgolini N., Silvano M., Hagan R., Correia R., Alves P., Clarke C., Roldao A., Isidro I. Published in: Biotechnology & Bioengineering, 2023, ISSN 1097-0290 Publisher: Wiley Online Library DOI: 10.22541/au.167121909.92795915/v1 Dissecting insect cell heterogeneity during influenza VLP production using single-cell transcriptomics Author(s): Marco Silvano, Nikolaus Virgolini, Ricardo Correia, Colin Clarke, Ines A. Isidro, Paula M. Alves, Antonio Roldao Published in: Frontiers in Bioengineering and Biotechnology, vol. 11, 2023, ISSN 2296-4185 Publisher: Frontiers DOI: 10.3389/fbioe.2023.1143255 Comparison of Clinically Relevant Oncolytic Virus Platforms for Enhancing T Cell Therapy of Solid Tumors Author(s): Cervera-Carrascon V., Quixabeira DCA., Havunen R., Santos JM., Kutvonen E., Clubb JHA., Siurala M., Heiniö C., Zafar S., Koivula T., Lumen D., Vaha M., Garcia-Horsman A., Airaksinen AJ., Sorsa S., Anttila M., Hukkanen V., Kanerva A., Hemminki A. Published in: Molecular therapy oncolytics, vol.17, 2020, ISSN 2372-7705 Publisher: CellPress DOI: 10.1016/j.omto.2020.03.003 Development of a Syrian Hamster anti-PD-L1 monoclonal antibody enables adenoviral oncolytic immunotherapy modelling in an immunocompetent virus replication permissive setting. Author(s): Clubb J., Kudling T.V., Quixabeira D., Santos J., Havunen R., Heiniö C., Cervera-Carrascon V., Pakola S., Basnet S., Grönberg-Vähä-Koskela s., Kanerva A., & Hemminki A. Published in: Frontiers in Immunology, vol.14, 2023, ISSN 1664-3224 Publisher: Frontiers DOI: 10.3389/fimmu.2023.1060540 Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics Author(s): Charitidis F.T., Adabi E., Thalheimer F.B., Clarke C., Buchholz C.J. Published in: Molecular Therapy – Methods & Clinical Development, vol.23, 2022, ISSN 2329-0501 Publisher: CellPress DOI: 10.1016/j.omtm.2021.09.019 Subphysiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells Author(s): Ioanna Tzani, Craig Monger, Krishna Motheramgari, Clair Gallagher, Ryan Hagan, Paul Kelly, Alan Costello, Justine Meiller, Patrick Floris, Lin Zhang, Martin Clynes, Jonathan Bones, Niall Barron, Colin Clarke Published in: Biotechnology and Bioengineering, 117/8, 2020, Page(s) 2489-2503, ISSN 0006-3592 Publisher: Wiley - V C H Verlag GmbbH & Co. DOI: 10.1002/bit.27365 Systemic Delivery of Oncolytic Adenovirus to Tumors Using Tumor-Infiltrating Lymphocytes as Carriers Author(s): Santos J, Heiniö C, Quixabeira D, Zafar S, Clubb J, Pakola S, Cervera-Carrascon V, Havunen R, Kanerva A, Hemminki A. Published in: Cells, vol. 10 issue 5, 2022, ISSN 2073-4409 Publisher: MDPI DOI: 10.3390/cells10050978 Adenovirus Encoding Tumor Necrosis Factor Alpha and Interleukin 2 Induces a Tertiary Lymphoid Structure Signature in Immune Checkpoint Inhibitor Refractory Head and Neck Cancer Author(s): Clubb J.H.A., Kudling T.V., Heiniö C., Basnet S., Pakola S., Cervera Carrascón V., Santos J.M., Quixabeira D.C.A., Havunen R., Sorsa S., Zheng V., Salo T., Bäck L., Aro K., Tulokas S., Loimu V., Hemminki A. Published in: Frontiers in Immunology, vol. 13, 2022, ISSN 1664-3224 Publisher: Frontiers DOI: 10.3389/fimmu.2022.794251 Transcriptome analysis of Sf9 insect cells during production of recombinant Adeno‐associated virus Author(s): Nikolaus Virgolini; Ryan Hagan; Ricardo Correia; Marco Silvano; Sofia Fernandes; Paula M. Alves; Colin Clarke; António Roldão; Inês A. Isidro Published in: Biotechnology Journal, 2022, ISSN 1860-7314 Publisher: Wiley Online Library DOI: 10.1002/biot.202200466 Expression of Extracellular Vesicle PIWI-interacting RNAs over hiPSC-cardiomyocyte differentiation Author(s): Louro A.F., Virgolini N., Paiva M.A., Isidro I.A., Alves P.M., Gomes-Alves P., Serra M. Published in: Frontiers in Physiology, Vol 13, 2022, ISSN 1664-042X Publisher: Frontiers Research Foundation DOI: 10.3389/fphys.2022.926528 Local delivery of interleukin 7 with an oncolytic adenovirus activates tumor-infiltrating lymphocytes and causes tumor regression Author(s): Kudling T.V., Clubb J.H.A., Quixabeira D.C.A., Santos J.M., Havunen R., Kononov A., Heiniö C., Cervera-Carrascon V., Pakola S., Basnet S., Grönberg-Vähä-Koskela S., Arias V., Gladwyn-Ng I., Aro K., Bäck L., Räsänen J., Ilonen I., Borenius K., Räsänen M., Hemminki O., Rannikko A., Kanerva A., Tapper J., Hemminki A. Published in: OncoImmunology, vol. 11, issue 1, 2022, ISSN 2162-4011 Publisher: Landes Bioscience DOI: 10.1080/2162402x.2022.2096572 Gene expression analysis of adapted insect cells during influenza VLP production using RNA-sequencing Author(s): Silvano, M., Correia, R., Virgolini, N., Clarke, C., Alves, P.M., Isidro, I.A., Roldão, A. Published in: Viruses, vol- 14 issue 10, 2022, ISSN 1999-4915 Publisher: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/v14102238 Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics Author(s): Ioanna Tzani, Nicolas Herrmann, Sara Carillo, Cathy A. Spargo, Ryan Hagan, Niall Barron, Jonathan Bones, W. Shannon Dillmore, Colin Clarke Published in: Biotechnology and Bioengineering, 2021, ISSN 0006-3592 Publisher: Wiley - V C H Verlag GmbbH & Co. DOI: 10.1002/bit.27715 Searching for OpenAIRE data... There was an error trying to search data from OpenAIRE No results available
