A new generation of microbial expression hosts and tools for the production of biotherapeutics and high-value enzymes

Informations projet

Secreters

N° de convention de subvention: 813979

DOI

10.3030/813979

Projet clôturé

Date de signature de la CE 30 Août 2018

Date de début 1 Janvier 2019

Date de fin 30 Juin 2023

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total

€ 4 087 420,92

Contribution de l’UE

€ 4 087 420,92

Coordonné par

ACADEMISCH ZIEKENHUIS GRONINGEN
Netherlands

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (32)

Optimised redox-engineered strains

Optimised redoxengineered strains

IP protected by patents, publications including review written by ESRs

IP protected by patents publications including review written by ESRs

Supervisory Board of the network
Public engagement activities completed
List of common and critical parameters for specific productivity

List of common and critical parameters for specific productivity

Knowledge-based models of redox processes
Molecular models of redox processes
Local training completed
Overall recruitment

Overall recruitment; ESRs recruited & full report on recruitment process, including gender equality.

ESRs awarded PhDs

ESRs awarded PhDs date depends on local regulations

2nd generation strains with enhanced disulfide bond fidelity
Report on gender equality monitoring throughout project
Best strains for oxidative folding identified
prototype strains for enhanced disulphide bond formation
Training by research completed
Training of trainers completed
Project website live
Prototype strains with engineered stress responses
Strains with tested engineered capabilities for oxidative folding
Rotations and secondments completed
Report on outcome quality assurance throughout project
Minimised product heterogeneity
Recruitment symposium organized
Strains with tested robustness
Constructs for scFv, Fab and industrial enzymes
Optimised strains with minimal stress
ESR positions advertised
Live cell redox measurements
Network-wide training completed

Networkwide training completed

2nd generation strains with engineered stress responses
CyDisCo stress responses defined
Plan for future dual or joint doctoral degrees

Publications

Peer reviewed articles (12)

Production of neutralizing antibody fragment variants in the cytoplasm of E. coli for rapid screening: SARS-CoV-2 a case study

Auteurs: Tungekar Aatir A, Rosario Recacha & Lloyd W. Ruddock
Publié dans: Scientific Reports, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-023-31369-2

Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study

Auteurs: Klaudia Arauzo-Aguilera, Luisa Buscajoni, Karin Koch, Gary Thompson, Matthias Berkemeyer, Colin Robinson
Publié dans: Microbial Cell Factories, 2023, ISSN 1475-2859
Éditeur: BioMed Central
DOI: 10.1186/s12934-023-02111-4

Microbial protein cell factories fight back?

Auteurs: Lukas A. Rettenbacher; Klaudia Arauzo-Aguilera; Luisa Buscajoni; Angel Castillo-Corujo; Borja Ferrero-Bordera; Aliki Kostopoulou; Rafael Moran-Torres; David Núñez-Nepomuceno; Ayşegül Öktem; Arianna Palma; Beatrice Pisent; Martina Puricelli; Tobias Schilling; Aatir A. Tungekar; Jonathan Walgraeve; David Humphreys; Tobias von der Haar; Brigitte Gasser; Diethard Mattanovich; Lloyd Ruddock; Jan M
Publié dans: Trends in Biotechnology, Numéro 40 (5), May 2022, 2022, Page(s) 576-590, ISSN 0167-7799
Éditeur: Elsevier BV
DOI: 10.1016/j.tibtech.2021.10.003

Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond formation in yeast

Auteurs: Palma A, Rettenbacher LA, Moilanen A, Saaranen M, Pacheco-Martinez C, Gasser B, Ruddock L
Publié dans: Sci Rep, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-023-41375-z

So you want to express your protein in Escherichia coli?

Auteurs: Tungekar AA, Castillo-Corujo A, Ruddock LW
Publié dans: Essays Biochem, 2021, ISSN 0071-1365
Éditeur: Portland Press, Ltd.
DOI: 10.1042/ebc20200170

Refolding in the modern biopharmaceutical industry

Auteurs: Buscajoni L, Martinetz MC, Berkemeyer M, Brocard C
Publié dans: Biotechnol Adv, 2022, ISSN 0734-9750
Éditeur: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.108050

Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of Bacillus subtilis

Auteurs: Walgraeve J, Ferrero-Bordera B, Maaß S, Becher D, Schwerdtfeger R, van Dijl JM, Seefried M.
Publié dans: Microbiology Spectrum, 2023, ISSN 2165-0497
Éditeur: American Society for Microbiology
DOI: 10.1128/spectrum.01608-23

Highly efficient export of a disulphide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli

Auteurs: Klaudia Arauzo-Aguilera, Mirva J Saaranen, Colin Robinson and Lloyd W Ruddock
Publié dans: MicrobiogyOpen, 2022, ISSN 2045-8827
Éditeur: John Wiley and Sons Inc.
DOI: 10.1002/mbo3.1350

A quantitative interpretation of oxidative protein folding activity in Escherichia coli

Auteurs: Rettenbacher LA and von der Haar T
Publié dans: Microbial Cell Factories, 2022, ISSN 1475-2859
Éditeur: BioMed Central
DOI: 10.1186/s12934-022-01982-3

Control of COVID-19 Outbreaks under Stochastic Community Dynamics, Bimodality, or Limited Vaccination

Auteurs: Goldenbogen, B., Adler, S.O., Bodeit, O., Wodke, J.A.H., Escalera‐Fanjul, X., Korman, A., Krantz, M., Bonn, L., Morán‐Torres, R., Haffner, J.E.L.
Publié dans: Adv. Sci, 2022, ISSN 2198-3844
Éditeur: Wiley-VCH
DOI: 10.1002/advs.202200088

Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of Bacillus subtilis

Auteurs: Öktem A, Núñez-Nepomuceno D, Ferrero-Bordera B, Walgraeve J, Seefried M, Gesell Salazar M, Steil L, Michalik S, Maaß S, Becher D, Mäder U, Völker U, van Dijl JM.
Publié dans: Microbiol Spectr., 2023, ISSN 2165-0497
Éditeur: American Society for Microbiology
DOI: 10.1128/spectrum.01778-23

Efficient Production of Fc Fusion Proteins in the Cytoplasm of Escherichia coli: Dissecting and Mitigating Redox Heterogeneity

Auteurs: Tungekar AA, Ruddock LW
Publié dans: Int J Mol Sci., 2022, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232314740