Skip to main content

Synthetic biology-guided engineering of Pseudomonas putida for biofluorination

Objective

Nature has hardly evolved biochemical reactions involving fluorine (F), the most abundant halogen on Earth. Organic compounds containing F (fluorochemicals) are, however, extremely relevant from an industrial point of view. Fluoropolymers are the main fluorochemicals in the market worldwide, and are exclusively synthesized using chemical methods. Moreover, current fluorination technologies usually involve corrosive and toxic reagents that have a negative impact on the environment. Designing sustainable bioprocesses based on alternative and safer fluorinating agents from renewable substrates is thus a long-sought-after, yet unfulfilled goal. SinFonia proposes to engineer the metabolically-versatile bacterium Pseudomonas putida to execute biofluorinations for generating novel fluoropolymers from renewable substrates. P. putida KT2440, a non-pathogenic soil bacterium, serves as an ideal microbial platform for F-dependent biochemical reactions due to its extraordinary resistance to harsh and stressful operating conditions. SinFonia will exploit natural selection to enhance bioproduction through a smart strain engineering approach in which bacterial growth will be coupled to biofluorination. Our target compounds are a whole family of fluorinated polyesters with enhanced physicochemical and material properties, with uses as self-cleaning surfaces, low-surface-energy coatings, bio-based lubricants, membranes for fuel cells, and anti-fouling materials. The versatile P. putida strains engineered during the project can be easily adapted to synthesize other added-value fluorochemicals. Unlike chemical processes, the source of F in our system will be NaF, an inexpensive and safe salt, and sugars as the main carbon source. In-depth analysis of all the environmental and economic benefits of the new fluorination technology, and interactive communication of social benefits associated with target products, are essential components of SinFonia.

Field of science

  • /social sciences/economics and business/economics/sustainable economy
  • /engineering and technology/mechanical engineering/tribology/lubrication
  • /natural sciences/chemical sciences/organic chemistry
  • /engineering and technology/environmental engineering/energy and fuels/fuel cell

Call for proposal

H2020-NMBP-BIO-2018-two-stage
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

DANMARKS TEKNISKE UNIVERSITET
Address
Anker Engelundsvej 1 Bygning 101 A
2800 Kgs Lyngby
Denmark
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 811 921,39

Participants (12)

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany
EU contribution
€ 1 010 875
Address
Hofgartenstrasse 8
80539 Muenchen
Activity type
Research Organisations
Masarykova univerzita
Czechia
EU contribution
€ 435 287,50
Address
Zerotinovo Namesti 9
60177 Brno Stred
Activity type
Higher or Secondary Education Establishments
THE UNIVERSITY COURT OF THE UNIVERSITY OF ST ANDREWS
United Kingdom
EU contribution
€ 481 250
Address
North Street 66 College Gate
KY16 9AJ St Andrews
Activity type
Higher or Secondary Education Establishments
AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 443 965
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations
TARTU ULIKOOL
Estonia
EU contribution
€ 390 000
Address
Ulikooli 18
51005 Tartu
Activity type
Higher or Secondary Education Establishments
UNIVERSITE DU LUXEMBOURG
Luxembourg
EU contribution
€ 631 590
Address
2 Avenue De L'universite
4365 Esch-sur-alzette
Activity type
Higher or Secondary Education Establishments
BIOPLASTECH LTD
Ireland
EU contribution
€ 715 103,75
Address
Cashel Road Unit 9 Block C Cashel Business Centre
12 Dublin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOFACTION KG
Austria
EU contribution
€ 375 268,75
Address
Kundmanngasse 39/12
1030 Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ALTAR
France
EU contribution
€ 302 560
Address
5 Rue Henri Desbrueres Bio Cluster Genopole Campus
91030 Evry
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
IN SRL
Italy
EU contribution
€ 382 682,09
Address
Via Carducci 9A
33100 Udine
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
IFEU - INSTITUT FUR ENERGIE- UND UMWELTFORSCHUNG HEIDELBERG GGMBH
Germany
EU contribution
€ 373 000
Address
Wilckensstrasse 3
69120 Heidelberg
Activity type
Research Organisations
CHEMOURS NETHERLANDS BV
Netherlands
EU contribution
€ 595 000
Address
Baanhoekweg 22
3313 LA Dordrecht
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)