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Synthetic microbial consortia-based platform for flavonoids production using synthetic biology

Project description

DEENESFRITPL

It takes a (microbial) village

One of the reasons fruits, vegetables and whole grains help protect us against non-communicable diseases, such as heart disease, diabetes and cancer, has to do with plant metabolism. Flavonoids are secondary metabolites produced by many plants that help them combat oxidative stress and act as growth regulators. They have antioxidant and anti-inflammatory properties in people. Despite the tremendous interest in flavonoids, their industrial production is currently immature. In nature, they are produced via complex physical and chemical pathways involving trafficking of chemicals throughout plant compartments. To better mimic this and even open the door to the production of flavonoids not seen in nature, the EU-funded SynBio4Flav project is developing synthetic microbial communities with members genetically programmed to execute specific steps in the complex biosynthetic pathways.

Objective

This Project pursues the implementation a standardized pipeline for surrogate production of plant flavonoids in synthetic microbial consortia (SMCs) by means of standardization and systems-guided assembly of highly complex biological devices. Flavonoids are the more abundant and consumed group of phytonutrients, used in numerous applications including functional food & beverages, dietary supplements, cosmetics, and pharmaceuticals. Despite its growing demand, flavonoids production remains elusive to chemical synthesis and biotech-based approaches, thus current flavonoid market is constrained to the scarce plant-based sources. These compounds are synthetized in nature through complex pathways involving an intense chemicals trafficking through plant compartments. By facilitating component troubleshooting and re-usability—instead of optimizing a single whole-cell biocatalyst— SynBio4Flav will recreate such non-homogeneous scenario by breaking-down specific portions of the complexes and highly regulated biochemical routes between different microbial species, each of them genetically programmed to deliver an optimal output of the corresponding biosynthetic step(s) i.e. through a distributed catalysis engineered in a defined SMC. Enabling such novel approach, SynBio4Flav will push the existing boundaries of the synthetic biology by acting along the whole Synthetic Biology hierarchy abstraction, and remarkably, in those with high complexity level e.g. cell systems and microbial communities. By creating libraries of optimized cell systems programmed to deliver an optimal output, and novel synthetic biology tools for cell systems assembling into 3D SMCs, SysBio4Flav will reach a TRL5 in production of natural and new-to-nature glycosylated flavonoids. The durable output of SynBio4Flav will be a standardized platform containing hundreds of optimal cell systems for exploring the full combinatorial space of flavonoids biosynthesis, including thousands of new-to-nature analogues.

Fields of science

Keywords

Programme(s)

Topic(s)

Call for proposal

H2020-NMBP-TR-IND-2018-2020

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Sub call

H2020-NMBP-BIO-2018-two-stage

Funding Scheme

RIA - Research and Innovation action

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution
€ 1 704 071,25
Address
Calle serrano 117
28006 Madrid
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
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Other funding
€ 0,00

Participants (10)

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Last update: 13 June 2023

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Permalink: https://cordis.europa.eu/project/id/814650

European Union, 2023