Novel approaches to the generation of artificial spider silk superfibers

Project Information

ARTSILK

Grant agreement ID: 815357

DOI

10.3030/815357

Project closed

EC signature date 21 January 2019

Start date 1 May 2019

End date 31 October 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 000 000,00

EU contribution

€ 2 000 000,00

Coordinated by

KAROLINSKA INSTITUTET
Sweden

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (26)

Force-Induced Structural Changes in Spider Silk Fibers Introduced by ATR-FTIR Spectroscopy

Author(s): Mohammad Javad Jafari, Fredrik G. Bäcklund, Tina Arndt, Benjamin Schmuck, Gabriele Greco, Anna Rising, Andreas Barth, and Thomas Ederth*
Published in: ACS Appl. Polym. Mater., 2023, ISSN 2637-6105
Publisher: American Chemical Society
DOI: 10.1021/acsapm.3c01892

Properties of Biomimetic Artificial Spider Silk Fibers Tuned by PostSpin Bath Incubation

Author(s): Gabriele Greco, Juanita Francis, Tina Arndt, Benjamin Schmuck, Fredrik G Bäcklund, Andreas Barth, Jan Johansson, Nicola M Pugno, Anna Rising
Published in: Molecules, Issue 25, 2020, Page(s) 3248, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25143248

Doing What Spiders Cannot—A Road Map to Supreme Artificial Silk Fibers

Author(s): Jan Johansson, Anna Rising
Published in: ACS Nano, Issue 15/2, 2021, Page(s) 1952-1959, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.0c08933

Artificial and natural silk materials have high mechanical property variability regardless of sample size

Author(s): Greco G, Schmuck B, Mirbaha H, Rising A, Pugno NM.
Published in: Scientific Reports, Issue March 3, 2022, Page(s) 3507, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-07212-5

Engineered spider silk proteins for biomimetic spinning of fibers with toughness equal to spider dragline silks.

Author(s): Arndt T, Greco G, Schmuck B, Bunz J, Shilkova O, Francis J, Pugno NM, Jaudzems K, Berth A, Johansson J, Rising A.
Published in: Advanced Functional Materials, Issue March 25, 2022, ISSN 1616-3028
Publisher: Wiley-VCH GmbH
DOI: 10.1002/adfm.202200986

High-performance magnetic artificial silk fibers produced by a scalable and eco-friendly production method

Author(s): Gabriele Greco, Benjamin Schmuck, Lucia Del Bianco, Federico Spizzo, Luca Fambri, Nicola Maria Pugno, Sabino Veintemillas-Verdaguer, Maria Puerto Morales, Anna Rising
Published in: Advanced Composites and Hybrid Materials, Issue 7, 2024, ISSN 2522-0128
Publisher: Nature Springer
DOI: 10.1007/s42114-024-00962-y

An image-analysis-based method for the prediction of recombinant protein fiber tensile strength

Author(s): Bäcklund FG, Greco G, Schmuck B, Miranda GHB, Pugno NM, Rydén J, Rising A.
Published in: Materials, Issue Jan 18, 2022, Page(s) 708, ISSN 1996-1944
Publisher: MDPI Open Access Publishing
DOI: 10.3390/ma15030708

Tuneable Recombinant Spider Silk Protein Hydrogels for Drug Release and 3D Cell Culture

Author(s): Arndt T, Chatterjee U, Shilkova O, Francis J, Lundkvist J, Johansson D, Schmuck B, Greco G, Ekblad Nordberg Å, Li Y, Wahlberg LU, Langton M, Johansson J, Götherström C, Rising A.
Published in: Advanced Functional Materials, 2023, Page(s) 2303622, ISSN 1616-3028
Publisher: Wiley-VCH GmbH
DOI: 10.1002/adfm.202303622

Origin, structure, and composition of the spider major ampullate silk fiber revealed by genomics, proteomics, and single-cell and spatial transcriptomics

Author(s): Sumalata Sonavane, Sameer Hassan, Urmimala Chatterjee, Lucile Soler, Lena Holm, Annelie Mollbrink, Gabriele Greco, Noah Fereydouni, Olga Vinnere Pettersson, Ignas Bunikis, Allison Churcher, Henrik Lantz, Jan Johansson, Johan Reimegård, Anna Rising
Published in: Science Advances, Issue 10, 2024, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.adn0597

Liquid–Liquid Phase Separation Primes Spider Silk Proteins for Fiber Formation via a Conditional Sticker Domain

Author(s): Axel Leppert; Gefei Chen; Dilraj Lama; Cagla Sahin; Vaida Railaite; Olga Shilkova; Tina Arndt; Erik G. Marklund; David P. Lane; Anna Rising; Michael Landreh
Published in: Leppert , A , Chen , G , Lama , D , Sahin , C , Railaite , V , Shilkova , O , Arndt , T , Marklund , E G , Lane , D P , Rising , A & Landreh , M 2023 , ' Liquid-Liquid Phase Separation Primes Spider Silk Proteins for Fiber Formation via a Conditional Sticker Domain ' , Nano Letters , vol. 23 , no. 12 . https://doi.org/10.1021/acs.nanolett.3c00773, Issue 1, 2023, Page(s) 5836-5841, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.3c00773

Influence of experimental methods on the mechanical properties of silk fibers: A systematic literature review and future road map

Author(s): Greco G, Schmuck B, Jalali SK, Pugno NM, Rising A.
Published in: Biophysics Reviews, Issue 4, 2023, Page(s) 031301, ISSN 2688-4089
Publisher: AIP Publishing LLC
DOI: 10.1063/5.0155552

Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions

Author(s): Axel Leppert, Jianhui Feng, Vaida Railaite, Tomas Bohn Pessatti, Carmine P. Cerrato, Cecilia Mörman, Hannah Osterholz, David P. Lane, Filipe R. N. C. Maia, Markus B. Linder, Anna Rising, Michael Landreh
Published in: Journal of the American Chemical Society, Issue 146, 2024, Page(s) 19555-19565, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c06688

Biological Materials Processing: Time-Tested Tricks for Sustainable Fiber Fabrication

Author(s): Anna Rising, Matthew J Harrington
Published in: Chemical Reviews, Issue 123, 5,, 2023, Page(s) 2155–2199, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.2c00465

Structure–Function Relationship of Artificial Spider Silk Fibers Produced by Straining Flow Spinning

Author(s): Nathalie Gonska, Patricia A. López, Paloma Lozano-Picazo, Michael Thorpe, Gustavo V. Guinea, Jan Johansson, Andreas Barth, José Pérez-Rigueiro, Anna Rising
Published in: Biomacromolecules, Issue 21/6, 2020, Page(s) 2116-2124, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.0c00100

Mass Spectrometry of RNA-Binding Proteins during Liquid–Liquid Phase Separation Reveals Distinct Assembly Mechanisms and Droplet Architectures

Author(s): Cagla Sahin, Aikaterini Motso, Xinyu Gu, Hannes Feyrer, Dilraj Lama, Tina Arndt, Anna Rising, Genis Valentin Gese, B. Martin Hällberg, Erik. G. Marklund, Nicholas P. Schafer, Katja Petzold, Kaare Teilum, Peter G. Wolynes, Michael Landreh
Published in: Journal of the American Chemical Society, Issue 145, 2023, Page(s) 10659-10668, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c00932

Tyrosine residues mediate supercontraction in biomimetic spider silk

Author(s): Gabriele Greco, Tina Arndt, Benjamin Schmuck, Juanita Francis, Fredrik G. Bäcklund, Olga Shilkova, Andreas Barth, Nathalie Gonska, Gulaim Seisenbaeva, Vadim Kessler, Jan Johansson, Nicola M. Pugno, Anna Rising
Published in: Communications Materials, Issue 2/1, 2021, ISSN 2662-4443
Publisher: Nature Group
DOI: 10.1038/s43246-021-00147-w

High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

Author(s): Benjamin Schmuck, Gabriele Greco, Andreas Barth, Nicola M. Pugno, Jan Johansson, Anna Rising
Published in: Materials Today, 2021, ISSN 1369-7021
Publisher: Elsevier BV
DOI: 10.1016/j.mattod.2021.07.020

Sustainable Spinning of Artificial Spider Silk Fibers with Excellent Toughness and Inherent Potential for Functionalization

Author(s): Ruxia Fan, Katarina Knuuttila, Benjamin Schmuck, Gabriele Greco, Anna Rising, Markus B. Linder, A. Sesilja Aranko
Published in: Advanced Functional Materials, 2024, ISSN 1616-301X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202410415

Strategies for Making High-Performance Artificial Spider Silk Fibers

Author(s): Schmuck B, Greco G, Bohn Pessatti T, Sonavane S, Langwallner V, Arndt T, Rising A.
Published in: Advanced Functional Materials, 2023, ISSN 1616-3028
Publisher: Wiley-VCH GmbH
DOI: 10.1002/adfm.202305040

Impact of physio-chemical spinning conditions on the mechanical properties of biomimetic spider silk fibers

Author(s): Schmuck B, Greco G, Bäcklund FB, Pugno NM, Johansson J, Rising A.
Published in: Communications Materials, 2022, ISSN 2662-4443
Publisher: Springer Nature
DOI: 10.1038/s43246-022-00307-6

Regionalization of cell types in silk glands of Larinioides sclopetarius suggest that spider silk fibers are complex layered structures

Author(s): Sumalata Sonavane, Per Westermark, Anna Rising, Lena Holm
Published in: Scientific Reports, Issue 13, 2024, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-49587-z

Effects of Mini-Spidroin Repeat Region on the Mechanical Properties of Artificial Spider Silk Fibers

Author(s): Benjamin Schmuck, Gabriele Greco, Olga Shilkova, Anna Rising
Published in: ACS Omega, Issue 9, 2024, Page(s) 42423-42432, ISSN 2470-1343
Publisher: ACS Publications
DOI: 10.1021/acsomega.4c06031

Biomimetic Spider Silk by Crosslinking and Functionalization with Multiarm Polyethylene Glycol

Author(s): Viktors Romaņuks, Jēkabs Fridmanis, Benjamin Schmuck, Anna Līna Bula, Alons Lends, Kristine Senkane, Gundars Leitis, Sergejs Gaidukovs, Krisjanis Smits, Anna Rising, Gints Smits, Kristaps Jaudzems
Published in: Advanced Functional Materials, 2024, ISSN 1616-301X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202409487

Spidroin N-terminal domain forms amyloid-like fibril based hydrogels and provides a protein immobilization platform

Author(s): Arndt T, Jaudzems K, Shilkova O, Francis J, Johansson M, Laity PR, Sahin C, Chatterjee U, Kronqvist N, Barajas-Ledesma E, Kumar R, Chen G, Strömberg R, Abelein A, Langton M, Landreh M, Barth, Holland C, Johansson J, Rising A.
Published in: Nature Communications, 2022, Page(s) 1-14, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-32093-7

Artificial spider silk supports and guides neurite extension in vitro

Author(s): Magnus L. Hansson, Urmimala Chatterjee, Juanita Francis, Tina Arndt, Christian Broman, Jan Johansson, Mattias K. Sköld, Anna Rising
Published in: The FASEB Journal, Issue 35, 2022, ISSN 0892-6638
Publisher: Federation of American Societies for Experimental Biology
DOI: 10.1096/fj.202100916r

Native-like Flow Properties of an Artificial Spider Silk Dope

Author(s): Tina Arndt, Peter R. Laity, Jan Johansson, Chris Holland, Anna Rising
Published in: ACS Biomaterials Science & Engineering, Issue 7/2, 2021, Page(s) 462-471, ISSN 2373-9878
Publisher: ACS Publications
DOI: 10.1021/acsbiomaterials.0c01308