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CORDIS

Novel approaches to the generation of artificial spider silk superfibers

Project description

Spinning a better artificial spider-silk

Spider silk is a high-performance natural material, with significant potential to revolutionise the materials industry. Unfortunately, its production and further spinning into artificial spider-silk fibres is an extremely difficult task. Moreover, the current procedures for manufacturing spider silk do not allow the correct structures to form in the fibers. The EU-funded ARTSILK project will make use of recent technological discoveries and cutting-edge technologies to produce artificial spider-silk fibres that are equal to or even better than native spider silk, in both in terms of toughness and tensile strength.

Objective

Spider silk is Nature’s high performance material that has the potential to revolutionize the materials industry. However, production and spinning of artificial spider silk fibers are challenging, and current methods to produce silk fibers include denaturing conditions which prevent the silk proteins from assembling into fibers in the same complex way as native silk proteins do. In order to fulfill the potential of spider silk we need to increase our understanding of the silk formation process and decipher how protein folding and interactions relate to mechanical properties of the resulting silk fiber. Recent insights into the physiology and molecular mechanisms of the spinning process has made it possible to develop a biomimetic artificial spider silk spinning device (see our publications Andersson et al. Nat Chem Biol. 2017; Otikovs et al. Angew Chemie Int Engl Ed. 2017). We are, for the first time, able to spin artificial silk fibers in which the proteins adopt correct secondary, tertiary and quaternary structures.

The overall objective of ARTSILK is to build on these recent technical leaps and use state-of-the-art technologies to generate artificial silk fibers that are equal or superior to native spider silk in terms of toughness and tensile strength.

To reach the overall objective we will use the recently mapped spider genome, protein engineering and single cell RNA (ScRNA) sequencing to design novel silk proteins for fiber production. We will also study the relationship between protein secondary structure formation and fiber mechanical properties in order to decipher the ques that determine mechanical properties of the fiber. This knowledge will be important also for the basic understanding of how soluble proteins covert into b-sheet rich fibrils in, e.g. Alzheimer’s disease. Finally, we will use microfluidic chips to engineer the next generation spinning device and 3D-printing techniques to make reproducible three-dimensional structures of spider silk.

Host institution

KAROLINSKA INSTITUTET
Net EU contribution
€ 2 000 000,00
Address
Nobels Vag 5
17177 Stockholm
Sweden

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Region
Östra Sverige Stockholm Stockholms län
Activity type
Higher or Secondary Education Establishments
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Total cost
€ 2 000 000,00

Beneficiaries (1)