With the continuous increase in human population, one of the key global challenges is to secure that the necessary growing food production is performed sustainably. It is known that gut microorganisms play a pivotal role in the health and well-being of animals, and a balanced gut microbiota is essential for optimal food production. In the past, different strategies are being developed to modulate gut microbiomes aiming to improve food production. Feed additives have been proven effective at modulating microbiomes in many animal systems, although both producers scientific data confirm that their efficiency often exhibits large variation. The likely reason underlying such inconsistency is the very limited knowledge we have about the specific means of action of the additives. In the best case, feed additive or food producing companies might employ both in vitro and in vivo experiments to test the properties and benefits of the additive. However, the information capacity of these approaches is limited, as the in vivo response of microorganisms can be radically different from in vitro conditions, because the microorganisms of interest are under different physico-chemical conditions and might interact with hundreds of other microbial taxa as well as the host organism. Thus currently, the improvement of feed additives usually relies on rudimentary trial-error in which researchers are unable to gain a full understanding of the reasons behind the success or failure of the use of different additives. Consequently, the procedures to improve the feed additive products are inefficient, and it is unlikely that any truly optimal product can be found without drastically modifying the approach taken. HoloFood is overcoming these limitations by leveraging state-of-the-art laboratory and computational developments, to provide direct insights into the effects of the biomolecular interactions between feed additives, gut microorganisms and host animals in food production. This is being achieved by implementing a novel holo-omic framework, that principally exploits decreases in the price of generating ‘omic datasets, coupled with cutting-edge biomolecular and statistical methods. HoloFood is based on two observations:i) Animals and their associated microorganisms behave differently depending on the environment and developmental stage of the animal and ii) many biological processes of animals and their associated microorganisms are affected, or even conditioned, by the other partner of the symbiosis. This implies that the effect of any feed additive could be different depending on the genomic background and developmental stage of the animal, microbiome composition, and the production environment. Our framework consists of analysing the genomes, transcriptomes and metabolomes of both animals and their associated gut microorganisms, then linking this to animal health and physiology, as well as food production performance parameters. To showcase the potential of our solution in different food production systems, and measure the commercial and societal impact of our approach, HoloFood will perform commercial-scale trials for both poultry and salmon in which the biomolecular interactions between microorganisms and animals fed with different feed additives will be analysed in relation to food production parameters measuring the quantity, quality and safety of the food, as well as the sustainability of food production and animal welfare. By understanding the physiological effects of the specific biomolecular interactions that occur when administering different feed additives, HoloFood aims to optimise their use by tailoring their administration to the genomic background and gut microbiome of the animals, as well as the farming environment, thus being able to increase the efficiency of feed additives and thereby improving final food products.In
the future we believe that food production systems will be optimised through holo-omic approaches. Hence, HoloFood will act as a paradigm for approaches and in doing so foster the creation of jobs related to scientific research and the food sector, both directly with the production companies and associated industries. For achieving such ambitious goals, HoloFood brings together 11 partners from both academia and industry from 6 countries in Europe. Moreover, the activities of HoloFood will contribute towards achieving many of the challenges highlighted in the UN’s Sustainable Development Goals.